TiO₂ Mag Sepharose is magnetic beads designed for enrichment of phosphopeptides from tryptic digested protein samples
Magnetic beads for selective capture and enrichment of phosphopeptides
Easy to use - visible and dense magnetic beads
Simple protocol with elution conditions optimized for MS analysis
Parallel handling of samples - six samples can be processed in less than one hour
TiO2 Mag Sepharose magnetic beads are based on titanium dioxide (TiO2) chromatography and are designed to simplify capture and enrichment of phosphopeptides. TiO2 has high affinity for phosphopeptides and is an efficient method for enrichment of phosphopeptides from complex samples
TiO2 Mag Sepharose improves ease-of-use in sample preparation of phosphopeptides. The magnetic bead format has excellent properties for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of all bound target peptides
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel
The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).
By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.
This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.
Currently kits are available to:
• quantify and qualify NGS fragments. • quantify and qualify RNA. • quantify and qualify genomic DNA. • do Mutation Detection, also known as TILLiNG, a reverse genetics technique.
The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.
Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.
An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.
His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.
Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells.
Increase target protein yield and decrease degradation through reduced and simplified sample handling
Choose between several different formats for screening and preparative purification of histidine-tagged proteins
IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.
AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.
In contrast to conventional in-vitro diagnostic methods, AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.
The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.
Cytation 3 is an automated digital microscope that provides high quality cellular imaging up to 60x in slides, microplates, Petri dishes and cell culture flasks.
Cytation™ 3 cell imaging multi-mode system combines automated digital microscopy and conventional multi-mode microplate detection in one instrument. This unique patent pending design provides rich phenotypic cellular information with well-based quantitative data, bringing cell biology research to a new level.
Fully integrated solution for automated fluorescence and brightfield microscopy of slides, microplates and flasksTemperature control to 45°C, CO2/O2 gas control and orbital shakingPowerful yet easy to use software for efficient image capture and analysisEliminates inconveniences commonly associated with conventional microscopy
The modular platform can easily upgrade to add conventional multi-mode microplate detection, to provide well-based intensity data along with phenotypic information from the microscope.
This is a highly advanced programmable pneumatic digital dispenser. It can store eight programmes and it can partially compensate for viscosity variation.
The dispenser features new digital controller technology with a clear digital display for a simultaneous overview of all parameters.
The Drifton 206-DF dispenser can store eight programmes in the controller’s memory and when it is programmed, it will adjust the pneumatic pressure automatically as the liquid becomes thicker or thinner.
The dispenser can partially compensate for variation in the dispensed medium.
Remember to order an air fitting to go with this one-component dispenser.
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs.
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. SuperScript® III RT features:
Thermostability—half-life of 220 min at 50°C
Yield—reduced RNase H activity for more full-length cDNA
Specificity—full activity at 50°C for increased specificity with gene-specific primers
Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3´ and 5´ RACE
Porvair Sciences will present its complete range of automation friendly microplates and equipment at European Lab Automation 2012 in Hamburg, Germany in partnership with long-term collaborator, KBiosystems.
Visitors to the Porvair / KBio stand at ELA 2012 will be able to see the Universal Robotic Manifold plus a growing range of high quality reagent reservoirs. The Universal Robotic Manifold is designed to be easily assembled and disassembled by robotic manipulators, thus enabling productive automation of SPE or DNA clean-up procedures. Able to accommodate collection plates from 14mm - 44mm in height and adaptable for working with short, medium and long skirts and drip directors, the Universal Robotic Manifold can operate with many different brands of filter plate and collection trays. Supplied with an integral vacuum gauge, the Manifold provides complete control of vacuum pressure ensuring plates can be processed with high reproducibility.
Combining uncompromising high quality with an affordable price - Porvair Sciences will display its extensive range of deep well, shallow well, storage, assay, SPE, protein precipitation and filtration microplates precisely manufactured to comply with ANSI/SBS dimensions ensuring complete compatibility with all automated sample handling systems, microplate readers and washers.
Also on display will be the Ultravap RC - Porvair's all new robot-compatible nitrogen blow down station. For many years, Porvair Sciences has led the field in nitrogen blow down evaporation. This new version of Porvair's most popular sample concentrator makes it even easier to integrate an automatic dry down step into your automated liquid handling protocol.
rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.
rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.
•Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
•Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
•High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
•High binding capacity such as Protein A/Protein G GraviTrap ~ 35 mg human IgG/column.
rProtein A/Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein A and protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
Solentim has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development.
Solentim, the developer of tools for shortening the upstream cell line development workflow, has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development. The new product can be used to quickly and reliably screen hundreds of plates from limited dilutions via automated image capture and analysis. By offering unmatched speed, Cell Metric™ rapid is ideal for large-scale projects investigating the optimal conditions for cell growth, processing many thousands of clones per day with walk-away ease of use. It will be launched and displayed at the 2nd Annual Cell Culture and Bioprocessing Conference in London (4-5 November), while more details can also be found on the Solentim website.
Using Cell Metric™ rapid, images from an entire 96-well plate can be captured in just 45 seconds, offering a more than three-fold improvement over the standard Cell Metric™. Clear, high resolution images are captured right up to the well edge to generate accurate, comprehensive and reproducible results. This makes it ideal for a wide range of analyses including confluence determination, clone selection and colony growth formation. The Cell Metric™ rapid can also integrate with Solentim’s well-proven, temperature-controlled microplate stacker and loader. This space-saving system holds a batch of 10 plates and has been designed to increase throughput as part of a workflow that reduces user intervention, maximising the use of resources and time. To achieve the highest levels of automation, the Cell Metric™ rapid can integrate with a wide variety of third-party robot arms, to form a completely automated robotic work cell.
Cell Metric™ rapid joins a growing product portfolio that offers market-leading clone imaging with significantly better optics and automated detection capabilities than previous generation products. Existing users have reported shaving up to three months off the development of a new cell line when using the Cell Metric™, while the accuracy provided by the system’s high resolution, whole-well imaging maximises the number of single cells that can be positively identified as clonal.
Aaron Figg, Managing Director of Solentim commented, “Automated analysis systems have the potential to significantly improve commercial cell line development at every stage of the process, from calibration of the cell dispensing methods and liquid handling steps, to the clone selection and right through to the optimisation of growth conditions and scale-up ready for manufacture. Our customers continue to report significant improvements in their process quality, time and cost savings from incorporating Cell Metric™ into their workflows.”
Revolutionise your performance by transforming your conventional, batch dynamic light scattering system into the stable, automated DynaPro™ Plate Reader. The DynaPro Plate Reader incorporates state of the art technology, providing superior filter-free high throughput dynamic light scattering (DLS or QELS) performance. The non-invasive technology measures samples directly from industry-standard microplates (96, 384, or 1536 well plates) using as little as 4 microliters of sample per well. After the plate is loaded into the DynaPro, the data are collected automatically, in an unattended manner, by the easy-to-use DYNAMICS® software, which supports full integration with industry leading liquid handling robotics. Once the data are collected the samples can be recovered and the plates discarded – no cleaning required!
Save valuable time and increase your productivity by factor 10 or more!
All in all, the DynaPro DLS Plate Reader will help you increase your productivity by a factor of 10 or more when sizing biomolecules and other nanoparticles compared to conventional batch DLS systems. With the DynaPro Plate Reader, you can perform the classic DLS applications but with more confidence. Samples can be loaded in duplicate or triplicate, and scanned several times by the Plate Reader, providing you with data that are a true measure of sample repeatability and reproducibility. Due to the low sample volumes and automated capability, these experiments can be performed in less time and without the hassles of conventional batch DLS. Moreover, because the DynaPro Plate Reader can operate in an unattended fashion, there is no additional ‘cost’ – your valuable time – associated with exploring a much larger number of solution conditions or protein or other nanoparticle constructs. The DynaPro can perform accelerated stability, thermal melting, and temperature studies of multiple samples in parallel.
New: The DynaPro Plate Reader is now available with Extended Temperature Control and On-board Camera! The onboard camera in the DynaPro Plate Reader II can automatically acquire and store images of each well, giving scientists a new dimension of data acquisition, and instant insight into the behavior of their samples.
LentiKat’s offers an unusual investment opportunity
LentiKat’s owns, manufactures and distributes a patented immobilization into lentil-shaped Lentikats Biocatalysts. The unique lentil-like shape of the Lentikats Biocatalyst ensures unlimited diffusion of substrate(s) into any part of the matrix, while still retaining a convenient size for easy separation from the reaction medium. They find application in the Pharma & Food industry (including bio-based chemicals), Wastewater treatment as well as Biofuels. more...
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. more