TiO₂ Mag Sepharose is magnetic beads designed for enrichment of phosphopeptides from tryptic digested protein samples
Magnetic beads for selective capture and enrichment of phosphopeptides
Easy to use - visible and dense magnetic beads
Simple protocol with elution conditions optimized for MS analysis
Parallel handling of samples - six samples can be processed in less than one hour
TiO2 Mag Sepharose magnetic beads are based on titanium dioxide (TiO2) chromatography and are designed to simplify capture and enrichment of phosphopeptides. TiO2 has high affinity for phosphopeptides and is an efficient method for enrichment of phosphopeptides from complex samples
TiO2 Mag Sepharose improves ease-of-use in sample preparation of phosphopeptides. The magnetic bead format has excellent properties for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of all bound target peptides
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel
Today, embryonic stem cells (ESC) and induced pluripotent stem cells (iPS) represent the most significant types of pluripotent stem cells (PSC).
In the beginning, ESC were cultured on feeder cell layers, primarily murine embryonic fibroblasts (MEF), in non-defined culture media containing fetal calf serum (FCS). Later on, FCS would be replaced by somewhat more defined serum replacements and the introduction of extracellular matrix (ECM) coatings supporting self-renewal of ESC allowed for feeder-free culture using feeder-cell conditioned medium. However, the culture process remained time-consuming, laborious and poorly defined.
In recent years PSC-culture has experienced significant technical progress. Chemically defined media compositions became a standard, followed by the first xeno-free defined formulations. However, all these culture systems still depended on non-defined, xenogenic and poorly standardized ECM preparations – a contradiction in terms when using defined / xeno-free media. Importantly, research recently overcame this hurdle, identifying natural as well as synthetic ECM molecules and peptides that support undifferentiated PSC-expansion. Scientists are now capable of completely defined / humanized derivation and culture of hPSC.
Despite these recent technical advancements, the established hPSC culture systems still share unfavourable properties. Most of them use supra-physiologically high amounts of growth factors and / or contain substances purified from human or animal origin. In addition animal-derived and / or non-defined ECM is used. The PromoCell hPSC Growth Medium DXF consequently eliminates these disadvantages:
Chemically defined / xeno-free complete culture system
No human / animal derived components
Physiologically low in growth factors
This optimal culture environment allows for a well-controlled culture process, consistent and reproducible performance, robust support of pluripotency and improved cloning efficiency.
In combination with the extracellular matrix hPSC-ECM DXF and the hPSC Dissociation Buffer DXF, PromoCell provides a defined and xeno-free complete culture system for human pluripotent stem cells.
The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level.
The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level. These rigid wall stirred-tank single-use vessels have been specifically designed for high cell density fermentation with bacteria, fungi and yeast. BioBLU f vessels can be used with the Eppendorf DASbox® and DASGIP® Parallel Bioreactor Systems.
Compared to cell culture applications, fermentation processes require much higher kLa values for proper mass transfer as well as suitable heating and cooling options. Proven stirred-tank design, powerful overhead drives featuring Rushton-type impellers and innovative baffles for cooling make the BioBLU f vessels achieve these demands.
Reliable scalability through industrial design
Sealed magnetic overhead drives with encapsulated bearings and Rushton-type impellers for excellent mixing
High performance mass & heat transfer suitable for high cell density fermentation
Minimal set-up times and most easy handling
Liquid-free Peltier exhaust condenser
All wetted materials animal component free and USP Class VI certified
Working volumes: 65 – 250 mL (BioBLU 0.3f) and 250 mL – 1.25 L (BioBLU 1f)
Combine the advantages of single-use bioreactor technology with the reliable performance of conventional glass or stainless steel bioreactors for your fermentation – Discover the Eppendorf BioBLU f product line: www.eppendorf.com/BioBLUf
PlateMaster is an amazingly easy to use, accurate solution for high throughput pipetting of 96 and 384 well plates.
PlateMaster is an amazingly easy to use, accurate solution for high throughput pipetting of 96 and 384 well plates. Its compact and ergonomic design means it can be used anywhere and by anyone for fast, effortless and reproducible pipetting of 96 channels.
The manual 96-channel pipetting PlateMaster can easily be operated by everyone after an introduction of just 5 minutes. Being a manually controlled instrument there is no complicated and time-consuming programming. The operation is as easy and intuitive as with a manual pipette.
Fill a plate in a single dispense!
Filling of a 96-well plate requires less than 10 seconds. Loading of 384-well plates is possible without any problems in only four pipetting steps. For the first time high-throughput experiments can be performed by avoiding high costs and saving staff resources.
High Accuracy and Safety
By using high quality Pipetman tips an accuracy and precision level equalling an 8- or 12- channel pipette is achieved in a single motion. Additionally, the risk of double-pipetting entire rows or columns is avoided.
A tailored fit
Through a variety of accessories such as adapters for loading 384-well plates, reagent reservoirs or sample heater blocks, the PlateMaster can be fully adapted to your needs.
For further information material please click here
The Celltron has been ideally adapted for use in CO2-incubators which allows researchers an entry into shaken cell culture, where they can profit from considerably improved cultivation results.
Your entry into shaken cell culture
Gentle magnetic drive
Minimal heat emission
The Celltron has been ideally adapted for use in CO2-incubators. This allows researchers all over the world an entry into shaken cell culture, where they can profit from considerably improved cultivation results. Due to the minimal power consumption of only 4 watts, there is practically no heat dissipation into the incubator. This guarantees constant temperatures in the incubator and ideal conditions for cell culture.
The magnetic touch controller is affixed to the exterior of the incubator cabinet, allowing you to retain an overview of the operational status at all times. Visual and audible alarms are generated externally at the controller and can be immediately perceived – and that translates into reliability for your cells!
An additional plus for the cell culture user is the certified antimicrobial surface of the shaker, which reliably kills microorganisms.
MATra is an easy-to-handle, very fast and highly efficient technology for optimized transfection of cell lines and primary cells.
Magnet Assisted Transfection (MATra) is an easy-to-handle, very fast (15 minutes!) and highly efficient technology to transfect cells in culture. With MATra, all types of nucleic acids from plasmid DNA or siRNA to oligonucleotides can be delivered – even into hard-to-transfect cell types such as neuronal or stem cells. Using this new technique, nucleic acids are in a first associated with specific magnetic nanoparticles. A magnetic force is applied then and the nucleic acids will be quantitatively and quickly drawn towards and delivered into the target cells leading to efficient transfection without disturbing the membrane architecture and without causing chromosomal damage. MATra can be used with adherent or suspension cells and is functional with or without serum. Compared to other transfection techniques (e.g. electroporation or lipofection), MATra results in extremely low cytotoxicity and is very cost-effective. MATra can also be adapted to high-throughput transfection assays using robotic stations and adapted protocols.
Our PromoFectin Transfection Reagent provides highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells. It consists of a non-liposomal polymer that is stable, water-soluble and free of any components of animal origin. PromoFectin compacts and protects the nucleic acid of interest, enables efficient transport into the cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor the delivery to the nucleus. Transfection with PromoFectin results in very low toxicity and is therefore ideally suited for the transfection of very sensitive cells, yielding optimal effects on gene expression. It can be used for adherent and non-adherent cells and the protocol does not require a medium change. Special cell type-specific PromoFectin variants are also available, which have been developed for an optimized transfection of e.g. endothelial cells (HUVECs, HDMECs etc.), hepatocytes, macrophages, dendritic or neuronal cells.
In addition, PromoKine provides a range of small-sized reporter plasmids (beta-galactosidase, luciferase, GFP, CAT, and SEAP) as well as fusion plasmids, which allow high-level expression of a target gene of interest with a GFP or luciferase fusion tag at its C- or N-terminus. The corresponding reporter assays (luciferase, beta-galactosidase & GFP) are also available.
Revolutionise your performance by transforming your conventional, batch dynamic light scattering system into the stable, automated DynaPro™ Plate Reader. The DynaPro Plate Reader incorporates state of the art technology, providing superior filter-free high throughput dynamic light scattering (DLS or QELS) performance. The non-invasive technology measures samples directly from industry-standard microplates (96, 384, or 1536 well plates) using as little as 4 microliters of sample per well. After the plate is loaded into the DynaPro, the data are collected automatically, in an unattended manner, by the easy-to-use DYNAMICS® software, which supports full integration with industry leading liquid handling robotics. Once the data are collected the samples can be recovered and the plates discarded – no cleaning required!
Save valuable time and increase your productivity by factor 10 or more!
All in all, the DynaPro DLS Plate Reader will help you increase your productivity by a factor of 10 or more when sizing biomolecules and other nanoparticles compared to conventional batch DLS systems. With the DynaPro Plate Reader, you can perform the classic DLS applications but with more confidence. Samples can be loaded in duplicate or triplicate, and scanned several times by the Plate Reader, providing you with data that are a true measure of sample repeatability and reproducibility. Due to the low sample volumes and automated capability, these experiments can be performed in less time and without the hassles of conventional batch DLS. Moreover, because the DynaPro Plate Reader can operate in an unattended fashion, there is no additional ‘cost’ – your valuable time – associated with exploring a much larger number of solution conditions or protein or other nanoparticle constructs. The DynaPro can perform accelerated stability, thermal melting, and temperature studies of multiple samples in parallel.
New: The DynaPro Plate Reader is now available with Extended Temperature Control and On-board Camera! The onboard camera in the DynaPro Plate Reader II can automatically acquire and store images of each well, giving scientists a new dimension of data acquisition, and instant insight into the behavior of their samples.
This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time.
From creating your gene to expressing your protein, Life Technologies offers a wide range of tools and expression systems that are easy to use, help enable rapid results, and offer high protein yields for every downstream application. This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time compared to using traditional methods. We even offer a complete service for the entire gene-to-protein workflow.
Request a copy of the Gene to Protein Brochure today by filling out the form here!
The Oligo PRO™ 96XT system provides fast, automated and economical size-based purity analysis of single stranded DNA and RNA oligonucleotides, and double stranded RNA interference (RNAi) products.
This dedicated analyzer is capable of analyzing 96 different samples simultaneously by parallel capillary gel electrophoresis with on-line, fixed wavelength UV absorbance detection. Preloaded and optimized separation and capillary conditioning methods simplify and streamline operation. The system can perform unattended analysis of up to 288 samples at once. Application-specific data analysis software provides automated or manual peak integration. The Oligo PRO™ is a proven technology used every day worldwide in production and research labs.
Key Features of the Oligo PRO™ System Include:
Low cost per sample.Unsurpassed throughput for oligo purity analysis!
Size-based, single nucleotide resolution from 10-mer to 80-mer lengths.
Easy to use and maintain with a low cost of ownership.
Minimal sample preparation and consumption.
DNA oligonucleotide and RNA oligonucleotide analysis.
New, native gel analysis for measuring purity and annealing efficiency of dsRNAi products.
A full line of available reagents for "turn-key" operation.
The Reference 2 is the successor to the highly successful Reference pipette, and enhances the range’s already high standards with a new design, reduced weight and operating forces and multi-channel versions.
As the premium product in Eppendorf’s pipette portfolio, the Reference 2 provides the most accurate results possible alongside robust, reliable handling and optimum user safety. These levels of security make the Reference 2 ideal for use with precious liquids, or in any application requiring extremely high levels of accuracy. When there can be no compromise on quality and reproducability, Eppendorf’s new Reference 2 is an ideal instrument for these liquid handling applications.
Filling of plates, gels, and reaction vessels
Phase extraction and removal of supernatants
The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid an accidential tip ejection a clear haptic feedback is provided.
The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids or other external conditions without the need for a full calibration.
BioPAT® SIMCA-online is a highly efficient software release for real-time multivariate statistical process monitoring and control of previously established Design Spaces.
Continuous real-time quality control and assurance is a highly desired state in biopharmaceutical manufacturing, which can be achieved by sophisticated process control strategies that use multivariate monitoring techniques to prevent or mitigate the risk of producing a poor quality product. Multivariate monitoring is based on models representing the relationship between parameters, whereas traditional process monitoring is based on univariate rules incapable of revealing correlation patterns and sometimes resulting in misleading conclusions.
BioPAT® SIMCA-online is a highly efficient software release for real-time multivariate statistical process monitoring and control of previously established Design Spaces based on current process parameters and (spectro) analytical data.
The software permits early detection of process deviations and provides user guidance for identifying potential root causes by displaying easy-to-understand graphics. This not only results in improved health, safety and environmental (HSE) performance, but also in enhanced control and assurance of the overall process and product quality.
More background reports, more interviews and as always, news from all the European regions: Discover our newly relaunched 2014 Autumn Issue, with in-depth reports on cancer immune therapy, biorefineries, biosimilars. more