Product of the Week

rProtein A/Protein G GraviTrap
Vergrößern

GE Healthcare

rProtein A/Protein G GraviTrap

rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.

rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.

•Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results. 

•Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs. 

•High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%. 

•High binding capacity such as Protein A/Protein G GraviTrap ~ 35 mg human IgG/column. 

rProtein A/Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein A and protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Contact Information:

GE Healthcare Europe GmbH
Life Sciences
Andrea Löhndorf, PhD, MBA
Regional Marketing Manager
cell: +49 (0)174 92 36 400
andrea.loehndorf@ge.com
www.gelifesciences.com

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PromoCell GmbH

Efficient testing of the pro- or anti-angiogenic capacity of substances and drugs demands for standardized and easy-to-use test systems that deliver reproducible data quickly.

PromoCell GmbH

PromoCell provides four different ready-to-use angiogenesis cell model systems. Each of them allows the simulation of angiogenesis processes, by detailed in vitro recapitulation of the biological events involved in de novo vessel formation and remodelling: proteolytic activity, migration, proliferation and tube formation.

The PromoCell 3D Angiogenesis Kit examines all process together. It contains a collagen matrix with embedded endothelial cell spheroids and arrives ready-to-use in your lab. The only thing you need to do in order to start the assay is to add your test substance. Following proper stimulation de novo vessel formation occurs within two days, visible by sprouting of tubes into the surrounding matrix. The number and length of the sprouts corresponds to the pro- or anti-angiogenic potency of the test substance. Evaluation of the results is straightforward and easy, and staining procedures are not necessary.

To investigate the separate events of angiogenesis in a simulated in vitro environment, the PromoCell Transmigration Kit, the PromoCell Invasion Kit and the PromoCell Planar Migration Assay are useful platforms.

The PromoCell Transmigration Kit takes advantage of the ability of endothelial cells to migrate along cytokine gradients in a concentration-dependent manner, allowing for the measurement of the chemotactic potential of a test substance.

The PromoCell Invasion Kit is suitable for in vitro simulation of the proteolytic activity of endothelial cells as well as investigating directed migration. Hence, the impact of test substances on one or both of the aforementioned parameters can be easily monitored.

The PromoCell Planar Migration Assay is a 2D test platform for rapid evaluation of the influence of test substances on the migratory response of endothelial cells.


Contact Information:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

Germany

+49 (0)6221-649340

info@promocell.com

www.promocell.com

CANDOR

ReadyTector is an easy, quick and clear all-in-one detection solution for Western blotting.

Candor Bioscience GmbH

All-in-one solution for Western blots

The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously. 

The only additional step is washing with the specific ReadyTector Wash Buffer.

ReadyTector for Western blotting is easy, quick and clear:

  • easy – very simple procedure
  • quick – very fast procedure with very quick results
  • clear – no background

The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.


Contact:

Candor Bioscience GmbH

Simoniusstrasse 39

88239 Wangen, Germany

Tel.: +49(0)-7522-7952-70

Fax: +49(0)-7522-7952-729

info@readytector.com

www.readytector.com

Pall Life Sciences

96-well filter plates pre-filled with a family of chromatography sorbents for sorbent screening, or to optimize conditions in high throughput mode for protein purification.

Pall Life Sciences

AcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with Pall chromatography sorbents, to enable early chromatography sorbent screening for protein purification. Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption. Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using Pall 1 mL or 5 mL PRC prepacked columns filled with the same sorbents. 

AcroPrep ScreenExpert plate packages offer:

Flexibility: One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents or two optimization plates: one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent - You select your preference.
Easy Scale Up: Perform Design of Experiments (DoE) in 96-well plates and transfer to column chromatography. 1 mL or 5 mL PRC pre-packed columns filled with relevant sorbents allow convenient verification and small preparative work.
Minimized Sample Consumption: 50 μL of sorbent per well, allowing small sample usage. 1 mL well volume and 100 to 500 μL working volume.
Convenience: A disposable format in a convenient individual package. Compatible with manual multi-well plate vacuum manifold or robotic workstation-assisted operations.


Click here to learn more.

Biological Industries

Isolate, grow, maintain and differentiate human mesenchymal stem cells from various sources with a complete, optimized culture system.

Biological Industries

Biological Industries offers a full product portfolio, including stem cell culture media, freezing media, attachment solution, cell dissociation solutions and initial differentiation kits. 

Isolation and Culture

MSC NutriStem® XF line and its supporting reagents are optimized for human mesenchymal stem cells from a variety of tissue sources and promise optimal growth and maintenance of hMSC, high proliferation rate, superior maintenance of cell characteristics, fibroblast-like morphology maintenance and long-term growth

Differentiation

MSC GoTM Osteogenic, Adipogenic and Chondrogenic  XF series offer a complete system for the evaluation and  reliable induction of hMSC from various sources into adipocytes, chondrocytes and osteoblasts/osteocytes. The kits are designed to be user friendly with all the growth factors and supplements necessary for the direct differentiation of hMSC

All products are completely free of serum and non-human derived components, hence:

  • Eliminate contagious health risks from viral agents, mycoplasma, and prions
  • Eliminate lot-to-lot variability
  • Limit immunological reactions once cells are transplanted
  • Suitable for clinical applications
  • Ensure reliable performance
  • Eliminate the drawbacks of unwanted background differentiation and interruption in the cell metabolism 


For more details please visit: www.bioind.com/mesenchymal-stem-cells

Or contact us: info@bioind.com

Life Technologies

This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time.

Life Technologies

From creating your gene to expressing your protein, Life Technologies offers a wide range of tools and expression systems that are easy to use, help enable rapid results, and offer high protein yields for every downstream application. This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time compared to using traditional methods. We even offer a complete service for the entire gene-to-protein workflow.

Request a copy of the Gene to Protein Brochure today by filling out the form here!

Or download the PDF version here!


Contactinformation for GeneArt® products and services:

Email: geneartsupport@lifetech.com

Europe: Tel: +49 (0)941 942 76100 Fax: +49 (0)941 942 76780

America and Asia: Tel: 800 955 6288 option 4/4/1

Wyatt Technology

The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards.

Wyatt Technology Corporation

The µDAWN™  Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the µDAWN is similar to the well-established miniDAWN™ TREOS™, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.

Distinctly from the miniDAWN, the µDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the µDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.

The µDAWN and its companion Optilab® UT-rEX™ Refractive Index Detector interface to most standard UHPLC systems. As these instruments are based on the advanced technology of the miniDAWN TREOS and Optilab T-rEX, respectively, users may be confident in the underlying capabilities cited in thousands of peer-reviewed publications

The µDAWN may also be used in microbatch (off-line) mode or in conjunction with a Calypso II CG-MALS system for characterization of macromolecular interactions: affinity and absolute stoichiometry, label-free and immobilization-free, as well as second virial coefficients, cross virial coefficients, average molar masses and sizes.

Contact: 

Wyatt Technology Europe GmbH

Dr. Thomas Jocks | Scientific Marketing

Hochstrasse 18 | 56307 Bernbach | Germany

Tel.: +49(0)2689-925-215 | Fax: +49(0)2689-925-299

Web: www.wyatt.EU | Email: thomas.jocks@wyatt.EU

PromoCell

Today, embryonic stem cells (ESC) and induced pluripotent stem cells (iPS) represent the most significant types of pluripotent stem cells (PSC).

PromoCell GmbH

In the beginning, ESC were cultured on feeder cell layers, primarily murine embryonic fibroblasts (MEF), in non-defined culture media containing fetal calf serum (FCS). Later on, FCS would be replaced by somewhat more defined serum replacements and the introduction of extracellular matrix (ECM) coatings supporting self-renewal of ESC allowed for feeder-free culture using feeder-cell conditioned medium. However, the culture process remained time-consuming, laborious and poorly defined.

In recent years PSC-culture has experienced significant technical progress. Chemically defined media compositions became a standard, followed by the first xeno-free defined formulations. However, all these culture systems still depended on non-defined, xenogenic and poorly standardized ECM preparations – a contradiction in terms when using defined / xeno-free media. Importantly, research recently overcame this hurdle, identifying natural as well as synthetic ECM molecules and peptides that support undifferentiated PSC-expansion. Scientists are now capable of completely defined / humanized derivation and culture of hPSC.

Despite these recent technical advancements, the established hPSC culture systems still share unfavourable properties. Most of them use supra-physiologically high amounts of growth factors and / or contain substances purified from human or animal origin. In addition animal-derived and / or non-defined ECM is used. The PromoCell hPSC Growth Medium DXF consequently eliminates these disadvantages:

  • Chemically defined / xeno-free complete culture system
  • No human / animal derived components
  • Physiologically low in growth factors

This optimal culture environment allows for a well-controlled culture process, consistent and reproducible performance, robust support of pluripotency and improved cloning efficiency.

In combination with the extracellular matrix hPSC-ECM DXF and the hPSC Dissociation Buffer DXF, PromoCell provides a defined and xeno-free complete culture system for human pluripotent stem cells.

Contact:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

Germany

+49 6221 649 34 0

www.promokine.info

info@promokine.info

Pall

The Cadence Inline Concentrator simplifies concentrating solutions of biomolecules to an in-line, single-use, ready-to-use process.

PALL

The recirculation loop associated with tangential flow filtration (TFF) conventionally used for this process is eliminated. Pump and tube sizes are reduced, product recovery is maximized, and sensitive proteins are exposed to less shear. The Cadence Single-Use Inline Concentrator moves the product being processed through a proprietary design flow path in a pre-assembled module with all connections already in place and designed to couple to available standard tubing manifolds.

Typical applications include monoclonal antibodies, fractionated antibodies, recombinant proteins, other proteins and recombinant blood factors. The Cadence Single-Use Inline Concentrator is used to concentrate clarified bulk after fermentation, and to reduce the number or size of bags needed and increase product concentration to improve chromatography performance. 

Cost projections with BioSolve software show that, compared to conventional single-use TFF, the Cadence Single-Use Inline Concentrator provides a 31% cost of goods sold and a 66% capital investment savings per batch because of the elimination of the need for intermediate tanks, ability to use smaller chromatography columns, energy savings, and the ability to couple processes to reduce downtime.


For further information please click here.

Lee Products

Miniature component specialists LEE PRODUCTS have recently published a new EFS (Electro Fluidic Systems) technical handbook which provides a valuable resource of product information along with practical engineering reference material.

Lee Products

Lee Product’s three Electro-Fluidic Systems groups produce high quality miniature solenoid valves, micro-dispense valves, atomising and dispense nozzles, fixed and variable volume pumps, integrated fluidic manifolds, inert tubing and fluid control components and custom engineered designs.  These products are used in medical and scientific instrumentation, analytical / clinical chemistry, in-vitro diagnostics, drug discovery and ink jet printing applications.

This new 323 page EFS handbook includes detailed information on Lee’s wide range of solenoid valves and pumps along with their manifolds, tubing, accessories, and special products, plus useful engineering and general information.


More details and copies of the handbook are available from:

Mike Stott

Lee Products Ltd

3 High Street

Chalfont St. Peter

Gerrards Cross

Bucks, SL9 9QE

Tel: +44 (0) 1753 886664

Fax:+44 (0)1753 889588

sales@leeproducts.co.uk

www.leeproducts.co.uk

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package for parallel bioprocess control, which brings a fresh new look and a whole raft of new features.

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package forparallel bioprocess control. Iris 6 brings a fresh new look and a whole raft of new features to one of themost flexible and user friendly bioprocess control software programs available today. A special versionfor use with Multitron incubation shakers allows comprehensive process validation.

“Beginners will love the modern design and the easy navigation”, says Dr. Tony Allman, productmanager INFORS HT. A new ribbon menu provides quick access to the bioreactor and parametersettings. A variety of graphical displays such as correlation and bar graphs allow an easy interpretationof process data. The new version allows the user to annotate on-line graphics while a fermentation isin progress and to time-stamp samples for easy reference.

Advanced features such as the feedback control using sequences allow the experienced user toexplore all possibilities of bioprocess control. The seamless integration of both analysed and calculatedprocess parameters gives the users the possibility to achieve a combined record of all process data.“The implementation of off-line analysed parameters like OD or protein yield in the batch record file isgreat, now I can record all data in one single file”, says Dr. Michael Kaempf, Project Leader BioprocessDevelopment, EMPA (Switzerland). An enhanced reporter module makes creation of batch tickets, data export and printing both comprehensive and simple.

The Iris Parallel Bioprocess Control Software is available in 3 different packages depending on the type of equipment. The same software provides high quality data acquisition and sophisticated controlstrategies from shake flask up to pilot production.

All software packages can be qualified for validation purposes relating to FDA CFR Part 11, 21.


Contact:

Dr. Tony Allman, Product Manager Fermentation

Infors AG

Rittergasse 27

4103 Bottmingen

Switzerland

Phone: +41 (0)61 425 77 00

Fax: +41 (0)61 425 77 01

e-Mail: t.allman@infors-ht.com

Web: www.infors-ht.com

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No liability assumed, Date: 30.06.2015

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