rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.
rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.
•Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
•Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
•High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
•High binding capacity such as Protein A/Protein G GraviTrap ~ 35 mg human IgG/column.
rProtein A/Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein A and protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
With improvements to the product and above all with enhanced documentation, the new minilab presents a huge number of new research opportunities for any user.
A new edition of the "lab in a suitcase"
For many years the Zinsser minilab has been an important component as a teaching aid in chemistry lessons in schools and universities. Now, with improvements to the product and above all with enhanced documentation, the new minilab presents a huge number of new research opportunities for any user.
Even though in the modern fine chemical industry and in pharmaceutical research and development where the smallest possible sample and reagent volumes are used (from a few mls and µls), for practical teaching, the experimentation often takes place in the front of the whole lesson with the use of larger volumes - up to 100mls - in schools and universities.
Containing an innovative set of glass equipment, the Mini Lab „lab in a suitcase“ is now used in many schools in different countries. There is now a new manual available for Mini Lab which includes more than 40 experiments - from basic operations such as distillation, filtration etc to complex syntheses such as colorants as designed by Prof. Michael Schallies. The instructions for the equipment configuration are easy to understand and are explained by simple drawings. General tips for the implementation of single experiments, material notes as well as danger symbols for the reagents are also included in the test description. The expected experimental results are also described in detail and partly documented with pictures or graphics. In the appendix of the manual there are detailed explanations of the potential dangers and also safety tips.
Current Owners of the Mini Lab can have a new updated manual for a small nominal charge.
Zinsser Analytic GmbH develops and manufactures in Germany and supplies customized automation technology and laboratory accessories for the global market. The company has specialized in the supply of high end technological products and systems for automated analysis in the fine chemical industry and also biological research and development. The company has grown over the years reaching a turnover in the range of double-digit millions and has about 60 employees to date.
Sartorius Stedim Biotech, a leading global supplier of the biopharmaceutical industry, is introducing a brand-new, scalable range of single-use bags.
Its completely new developed product family Flexsafe enables theimplementation of single-use bioprocessing throughout all steps of drug manufacture, fromprocess development to production, in upstream and downstream – all using just the oneinnovative polyethylene film. The innovative concept of the Flexsafe family addresses key industryrequirements for future-proof single-use manufacturing of commercial vaccines and drugs.
Flexsafe is based on a multilayer, proprietary polyethylene (PE) film, called S80, and has beendeveloped in close collaboration with resin and film suppliers. A standardized cell growthassay has been used to optimize film formulation, determine the operating ranges for extrusion,welding and gamma-irradiation processes and to establish specifications and process controls.
Flexsafe ensures excellent and reproducible cell growth behavior of the most sensitive celllines. The optimization of the resin formulation, the complete control of raw materials, theextrusion process and the bag assembly guarantee lot-to-lot consistent cell growthperformance. With its robust 400 μm thick PE film, Flexsafe is the strongest and most flexiblebag currently on the market. The bags enable safe and easy-to-use operation, even in themost demanding applications such as liquid shipping and large-scale stirred bioreactors.
Furthermore, batch-to-batch consistent extractables and leachables profiles support drugmanufacturers throughout the entire lifecycle of modern biological treatments from clinicaldevelopment to commercial supply many years after launch. Users can gain assurance thattheir initial extractable and leachable qualification work and data remain valid every timethey operate their single-use Flexsafe bioprocess.
Assurance of supply, one of the most important aspects for manufacturers, is guaranteed as aresult of long-term contracts with suppliers and business continuity plans with defined safetystocks and global manufacturing capabilities including resin manufacturing.
„Flexsafe represents a completely new generation of single-use bags. They offer our customersconsistent cell growth as well as extraordinary robustness and flexibility. In addition asustainable supply chain and consistent film throughout the entire manufacturing process isguaranteed. Every single one of these benefits sets a new benchmark. Being unique in all ofthem makes us very proud”, stated Stefan Schlack, Senior Vice President Marketing andProduct Management at Sartorius Stedim Biotech.
Sartorius Stedim Biotech is launching Flexsafe RM bags (1L-200L) and small bags for validationpurposes first. Bags for single use bioreactors BIOSTAT STR (50-2000L) and additional applications such as storage, mixing, shipping freeze and thaw will be rolled out step by step.
AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.
In contrast to conventional in-vitro diagnostic methods, AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.
The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.
The ProdX software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient.
Seamless Integration of Product Inspection Devices
Whatever your role in product inspection management, you will have plenty of challenges to face in marketplaces that increasingly demand more for less.
ProdX Inspect data management software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient. ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
ProdX Savings Calculator
Calculate your time savings potential with ProdX
This Calculator runs through all costs involved in the setup and data collection processes. It calculates the amount you could save per year by comparing the time requirement of manual processes and the automated data collection process this software offers.
ProdX Quality Management & AssuranceThe innovative ProdX software automatically collects the critical inspection process data you need in one convenient location significantly reducing the need to interact directly with the Critical Control Points on your factory floor. This saves time and effort enabling quality operatives to focus on other issues.
ProdX Production Process Managment
ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
PromoKine offers a wide range of products for cell analysis including numerous kits for fast, convenient and sensitive measurement of cell viability/proliferation, cytotoxicity, cell death and apoptosis, cell stress and metabolism as well as reporter gene expression.
PromoKine’s Fluorometric Cell Viability Kits are based on resazurin or calcein-AM conversion while our Colorimetric Cell Viability Kits utilize reduction of different tetrazolium salts (e.g. MTT, XTT, WST-1 & WST-8), and the Bioluminescent Cell Viability Kits measure ATP levels as a marker for cell viability in a luciferase assay.
Our Cytotoxicity Kits quantify the amount of lactate dehydrogenase (LDH) or adenylate kinase (AK) released from damaged cells either colorimetrically or by using a luminometer.
PromoKine’s Apoptosis Assays include various kits for detection and quantitation of e.g., caspase activity, annexin V binding, DNA fragmentation or mitochondrial changes. Moreover, PromoKine provides Reporter Assay Kits (beta-galactosidase, luciferase, GFP) as well as a selection of small-sized reporter plasmids and fusion plasmids (target gene fused with GFP or luciferase gene).
In addition, you will find in our catalog and on our website a wide range of other interesting products for cell biology research such as numerous high-quality and economically priced recombinant proteins (e.g. caspases, kinases, cytokines, chemokines & growth factors), a variety of primary and secondary antibodies and ELISA kits as well as premium, user-friendly reagents and kits for optimal cell transfection, fluorescent staining of cell structures, labeling of biomolecules (proteins, antibodies and nucleic acids) and the detection, elimination and prevention of mycoplasma and bacterial contamination in cell culture.
ReadyTector is an easy, quick and clear all-in-one detection solution for Western blotting.
All-in-one solution for Western blots
The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously.
The only additional step is washing with the specific ReadyTector Wash Buffer.
ReadyTector for Western blotting is easy, quick and clear:
easy – very simple procedure
quick – very fast procedure with very quick results
clear – no background
The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.
The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes.
1. Principle of Operation
The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes. The sample is passed through the membrane by pushing the sample back and forth between two syringes.
2. Temperature Control
The entire LiposoFast-Basic can be immersed in a water bath for use with high transition temperature lipids or heat sensitive compounds.
The optional LiposoFast-Stabilizer was designed to simplify the repetitive use of the LiposoFast-Basic as well as the extrusion of highly concentrated samples. It accommodates both 0.5mL and 1.0mL syringes.
4. Free Trial
This instrument is available for a free trial. Simply contact us and we can send it to you. If after the trial you are not satisfied with it or it does not suit your research, simply send it back.
Today, embryonic stem cells (ESC) and induced pluripotent stem cells (iPS) represent the most significant types of pluripotent stem cells (PSC).
In the beginning, ESC were cultured on feeder cell layers, primarily murine embryonic fibroblasts (MEF), in non-defined culture media containing fetal calf serum (FCS). Later on, FCS would be replaced by somewhat more defined serum replacements and the introduction of extracellular matrix (ECM) coatings supporting self-renewal of ESC allowed for feeder-free culture using feeder-cell conditioned medium. However, the culture process remained time-consuming, laborious and poorly defined.
In recent years PSC-culture has experienced significant technical progress. Chemically defined media compositions became a standard, followed by the first xeno-free defined formulations. However, all these culture systems still depended on non-defined, xenogenic and poorly standardized ECM preparations – a contradiction in terms when using defined / xeno-free media. Importantly, research recently overcame this hurdle, identifying natural as well as synthetic ECM molecules and peptides that support undifferentiated PSC-expansion. Scientists are now capable of completely defined / humanized derivation and culture of hPSC.
Despite these recent technical advancements, the established hPSC culture systems still share unfavourable properties. Most of them use supra-physiologically high amounts of growth factors and / or contain substances purified from human or animal origin. In addition animal-derived and / or non-defined ECM is used. The PromoCell hPSC Growth Medium DXF consequently eliminates these disadvantages:
Chemically defined / xeno-free complete culture system
No human / animal derived components
Physiologically low in growth factors
This optimal culture environment allows for a well-controlled culture process, consistent and reproducible performance, robust support of pluripotency and improved cloning efficiency.
In combination with the extracellular matrix hPSC-ECM DXF and the hPSC Dissociation Buffer DXF, PromoCell provides a defined and xeno-free complete culture system for human pluripotent stem cells.
The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level.
The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level. These rigid wall stirred-tank single-use vessels have been specifically designed for high cell density fermentation with bacteria, fungi and yeast. BioBLU f vessels can be used with the Eppendorf DASbox® and DASGIP® Parallel Bioreactor Systems.
Compared to cell culture applications, fermentation processes require much higher kLa values for proper mass transfer as well as suitable heating and cooling options. Proven stirred-tank design, powerful overhead drives featuring Rushton-type impellers and innovative baffles for cooling make the BioBLU f vessels achieve these demands.
Reliable scalability through industrial design
Sealed magnetic overhead drives with encapsulated bearings and Rushton-type impellers for excellent mixing
High performance mass & heat transfer suitable for high cell density fermentation
Minimal set-up times and most easy handling
Liquid-free Peltier exhaust condenser
All wetted materials animal component free and USP Class VI certified
Working volumes: 65 – 250 mL (BioBLU 0.3f) and 250 mL – 1.25 L (BioBLU 1f)
Combine the advantages of single-use bioreactor technology with the reliable performance of conventional glass or stainless steel bioreactors for your fermentation – Discover the Eppendorf BioBLU f product line: www.eppendorf.com/BioBLUf
PlateMaster is an amazingly easy to use, accurate solution for high throughput pipetting of 96 and 384 well plates.
PlateMaster is an amazingly easy to use, accurate solution for high throughput pipetting of 96 and 384 well plates. Its compact and ergonomic design means it can be used anywhere and by anyone for fast, effortless and reproducible pipetting of 96 channels.
The manual 96-channel pipetting PlateMaster can easily be operated by everyone after an introduction of just 5 minutes. Being a manually controlled instrument there is no complicated and time-consuming programming. The operation is as easy and intuitive as with a manual pipette.
Fill a plate in a single dispense!
Filling of a 96-well plate requires less than 10 seconds. Loading of 384-well plates is possible without any problems in only four pipetting steps. For the first time high-throughput experiments can be performed by avoiding high costs and saving staff resources.
High Accuracy and Safety
By using high quality Pipetman tips an accuracy and precision level equalling an 8- or 12- channel pipette is achieved in a single motion. Additionally, the risk of double-pipetting entire rows or columns is avoided.
A tailored fit
Through a variety of accessories such as adapters for loading 384-well plates, reagent reservoirs or sample heater blocks, the PlateMaster can be fully adapted to your needs.
For further information material please click here
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR) Assay allows you to detect the activities of firefly and NanoLuc® luciferases in a single sample. more
LentiKat’s offers an unusual investment opportunity
LentiKat’s owns, manufactures and distributes a patented immobilization into lentil-shaped Lentikats Biocatalysts. The unique lentil-like shape of the Lentikats Biocatalyst ensures unlimited diffusion of substrate(s) into any part of the matrix, while still retaining a convenient size for easy separation from the reaction medium. They find application in the Pharma & Food industry (including bio-based chemicals), Wastewater treatment as well as Biofuels. more...
celares GmbH is a Berlin based drug delivery company focused on chemical modifications of biological drugs. We offer customized development services for international pharma and biopharma companies and have manufacturing capabilities for key starting materials used for the preparation of pharmaceutical drugs. For the intensification of our own sales activities we are looking for a sales manager. more...