Product of the Week

Revolutionary new sterility testing workstation Bioquell QUBE
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Bioquell

Revolutionary new sterility testing workstation Bioquell QUBE

Featuring proven bio-decontamination technology and flexible design, this new system offers significant efficiencies for cGMP pharmaceutical manufacturing.

By ensuring a controlled aseptic test environment, the Bioquell QUBE helps to minimise the risk of unnecessary, and costly, batch rejection due to false positive sterility test results.

The new workstation has been developed as a cost-effective, modular, barrier separation system. It incorporates Bioquell’s unique hydrogen peroxide vapour (HPV) technology to offer a rapid bio-decontamination cycle for fast and efficient transfers. HPV consistently delivers a high-level 6-log reduction in bioburden, validated by high quality HPV-optimised biological indicators. The Bioquell QUBE has been designed to accommodate a fully fitted or bench mounted sterility test unit, including the market leading Merck Millipore Steritest system. An EU GMP Grade A / ISO 5 compliant environment is ensured by both uni-directional airflow and unique isolator technology.

The intelligent modular design offers flexible configurations. If space is limited, a single module can be used. Conversely, if there is a need for high volume throughput, a three chamber workstation can be installed.

The Bioquell QUBE is the latest edition to the company’s portfolio of solutions for sterility assurance in cGMP pharmaceutical and biotechnology processes, backed by over 200 years of decontamination experience. Explore the new generation of hydrogen peroxide vapour (HPV) bio‑decontamination technologies from Bioquell at  www.bioquell.com/bioquell-qube/


Contact Information:


Tel.: +44 1264 835 835

info@bioquell.com

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.  The analyte specific reagents (ASRs) provide high specificity, convenience and flexibility in flow cytometry.  A new cell permeabilization reagent is also being offered. 

The new monoclonals include human anti-hemoglobin F, which recognizes a fetal hemogloblobin molecule; CD163 markers for anti-inflammatory signal and disease process research and CD64 markers, which react with Fc gamma receptors. Characterized, quality controlled, convenient, liquid reagents allow users to spend less time in sample preparation and more time on experiments.

Rounding out the line is a monoclonal for MNDA (myeloid nuclear differentiation antigen). All antibodies are available unconjugated or conjugated to a selection of fluorochromes. Intra-Cell™ concentrate for cell permeabilization is also being released.

Beckman Coulter Life Sciences offers an extensive line of human and non-human antibodies as well as a range of supporting reagents and immune monitoring products. The new antibodies are supplied in 1.0 mL of buffer, are ready to use and facilitate flexibility in experimental design.

“Convenience and specificity are important features for scientists,” said Mario Koksch, vice president and general manager for Beckman Coulter Life Sciences’ Flow Cytometry Business Unit. “These new antibodies are directed at important targets and will improve accuracy for researchers.”

A strategic partnership between the companies enables Beckman Coulter Life Sciences to further diversify their extensive flow cytometry reagent portfolio and Trillium Diagnostics to broaden the reach of their products in the global market.    


Contact Information:

Hillary Hunter

Beckman Coulter Life Sciences Public Relations

hillarythunter@beckman.com

www.beckmancoulter.com

GE-Healthcare

Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.

GE-Healthcare


  • Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex G-25 Medium. 
  • Flexible protocols allow runs by gravity flow or centrifugation. 
  • Easy to use and equilibrate; apply sample; elute. 
  • Preparation of the samples in the range of 1.0 to 2.5 ml by gravity flow and 1.75 to 2.5 ml with centrifugation. 
  • Desalt multiple samples in parallel with a desalting capacity > 90%. 
  • Recovery in the range 70 to >95%. 


The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.


Contact information:


Andrea Löhndorf, PhD, MBA
Marketing Manager EMEA
GE Healthcare Europe GmbH
Life Sciences
 
Mobil: +49(0) 174 92 36 400

andrea.loehndorf@ge.com

www.gelifesciences.com

Biometra

The Biometra GmbH, a company of the Analytik Jena AG, now offers illuminators with high-performance blue light LEDs for gel documentation purposes.

Biometra

Blue light transilluminators are a quite interesting alternative to UV tables as there is no risk of sample damage during illumination. This is important when samples shall be processed furthermore after gel documentation. Users also benefit from it as there is no risk of UV exposure. Blue light excitation is applicable for fluorescent dyes for nucleic acid or protein stains with excitation wavelength around 470 nm. Examples for compatible stains are: SYBR® Green, GelGreenTM, SYBR® Safe, SYBR® Gold or SYPRO® Ruby. The amber lid of the instrument allows the direct view to the fluorescent samples. There is no need for separate glasses. 
The new blue light illuminators are available in two versions: "BLstar 9", the handy solution for mini gels and "BLstar 16" for gels up to size of 16 cm x 20 cm.


Contact information:

Hanna Engelhardt

Media Relations Life Science


Tel: +49 36 41 77-73 55

Fax: +49 36 41 77-92 79

press-ls@analytik-jena.com

www.analytik-jena.com

www.biometra.com

PromoCell

Get large numbers of highly pure and fully functional M1 or M2 Monocyte-derived Macrophages (MDM) by simply plating PBMC in our PromoCell Macrophage Generation Medium DXF, which supports Macrophage differentiation with high efficiency and reliability.

PromoCell

The Macrophage Generation Medium DXF is available in two different versions: the M1 Macrophage Generation Medium DXF and the M2 Macrophage Generation Medium DXF. Each of them is specifically optimized for the directed differentiation of M1 or M2 macrophages, respectively. In addition, PromoCell has developed the Macrophage Detachment Solution DXF for gentle and efficient release of macrophages from the culture surface. 

As with all of our DXF series media, the Macrophage Generation Medium DXF is chemically defined / xeno-free and thus provides a controlled culture environment devoid of animal component-derived stimuli - a significant benefit, in terms of Monocytes and Macrophages standing for highly reactive immune cells.

Find our Macrophage Media and many more products in our new 2013/2014 catalog! Get your free copy: www.promocell.com/catalog

Contact information:

PromoCell GmbH

Phone: +49 6221-649 34 0 (USA/Canada: 1-866-251-2860)

info@promocell.com

www.promocell.com

Medline

Laboratory equipment specialists MEDLINE SCIENTIFIC has introduced a range of micropipettes which are designed to provide optimal ease of use and assure a high level of user comfort during operation, minimising the risk of repetitive strain injury (RSI).

Medline

The Medline micropipette is manufactured in Germany, is CE-marked for IVD use and factory calibrated to dispense accurately under normal European ambient conditions (20° C, 50% humidity and 101Kpa barometric pressure).  Individual micropipettes are supplied with a calibration and test certificate and they are tested twice and calibrated to conform to EN ISO 8655 and DIN 12650 standards.

Integral to the design of the variable volume pipette is an innovative soft grip and safety device on the thumb-controlled plunger. This feature prevents accidental volume changes during use facilitating maximum precision and accuracy.  The universal two-step plunger allows reverse pipette techniques to be performed and enables last drop dispensing. This further contributes to a high level of accuracy in use.

Medline micropipettes may be safely autoclaved at 120°C and 1.05bar/15Psi for 15 minutes.  Maintenance and adjustment are simple procedures as the user can readily re-calibrate the micropipette with the adjustment tool supplied.  An operating manual is included with each instrument.

There are eight variable models covering volume ranges from 0.1 to 5000µl.  A further fourteen fixed volume models compliment the range covering 5 to 10,000µl and 4 or 6 position bench racks are available for easy storage.             


More details on these new pipettors are available from:

Lee or Emma at: 

Medline Scientific Ltd

Chalgrove

Oxfordshire, OX44 7XZ

UK.

Tel: +44(0) 1865 892 987

mail@medlinescientific.com

http://www.medlinescientific.com/en/New-Products/Pipettors/View-all-products.html

Molecular Devices

Molecular Devices®, market leader in microplate reader technology for over 25 years, has announced the launch of its SpectraMax® i3 Multi-Mode Detection Platform with SpectraMax® MiniMax® Imaging Cytometer.

Molecular Devices

The system is available as a stand-alone reader with the option for users to upgrade to additional applications and detection modes such as cellular imaging with the SpectraMax® MiniMax™ Imaging Cytometer, and cartridges for Time-Resolved Fluorescence, Fluorescence Polarization, and AlphaScreen® assays. The highly sensitive instrument accommodates the budget and throughput needs of both small and large laboratories alike.

The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Patented user-exchangeable cartridge design expands the system's detection capability making it highly versatile, and able to offer application options far exceeding those of standard readers. The MiniMax Imaging Cytometer module adds first of its kind cellular imaging to a multi-mode detection platform, enabling fluorescence and bright field cellular imaging. Combining cellular imaging with microplate-based applications offers new ways for scientists to compress their workflows and increase efficiency. 

Using a combination of a flash lamp for spectral flexibility and light emitting diodes (LEDs) for excitation power, Molecular Devices has developed the patent-pending Spectral FusionTM Illumination to deliver wavelength flexibility whilst maximizing signal strength, ultimately increasing overall performance.

Data from the SpectraMax i3 System is captured and analysed using Molecular Devices' proprietary, industry-leading SoftMax® Pro Software. The newly updated interface and Spectral Optimization Wizard simplifies the workflow and provides powerful data analysis features, eliminating the need for training on additional software. The SpectraMax i3 System is also available for use in GMP and GLP labs when used with the SoftMax® Pro 6.3 GxP Microplate Data Compliance Software.

Klaus Lun, Vice President of Product Marketing at Molecular Devices commented: "This new platform builds on Molecular Devices' experience in life science detection instrumentation development and our commitment to bring additional utility to the microplate reader, further compressing the workflows for our customers. With the MiniMax Imaging Cytometer for cellular imaging, Molecular Devices introduces a new world of cell-based assay application possibilities to the microplate reader.  We will continue to expand the platform's functionality by adding new capabilities to the microplate reader through the introduction of application-specific detection cartridges in 2013."

Please visit our Homepage for more details

Contacts:

Molecular Devices

Carlene Whitehead

Tel: +44 (0) 118 944 4651

Email: Carlene.Whitehead@moldev.com

For more information about the complete product portfolio, visit: www.moleculardevices.com

Hamamatsu Photonics

Hamamatsu Photonics has released the ImagEM X2, a new electron multiplying (EM) CCD camera with even faster speed than previous ImagEM cameras.

Hamamatsu Photonics

The ImagEM X2, a completely redesigned camera featuring a back-thinned EM-CCD sensor, offers maximum speed and precision performance for low-light imaging.

The ImagEM X2 makes superfast exposures possible and has the sensitivity to provide visually pleasing and quantitatively meaningful images in a photon-starved environment. It delivers 70 frames/s at the full resolution of 512 x 512 pixels with a high signal-to-noise ratio, enabling quantitative high-speed, low-light imaging. When binning or a region of interest is selected, this new camera produces images at even higher frame rates (up to 1076 frames/s).

Additional features allow for optimized camera triggering and streamlined connectivity through IEEE 1394b. The ImagEM X2 also has improved overall signal-to-noise ratio, increased non-EM dynamic range, and built-in EM gain measurement and calibration functions. It also features a software-controllable shutter that prevents EM gain degradation and image lag. To prevent EM gain degradation, the shutter is closed when the built-in EM gain protection feature is enabled. In addition, closing the shutter when a user replaces a lens, for example, can prevent image lag.


Press contact:

Hamamatsu Photonics Deutschland GmbH

Eva-Maria Tomic

Arzbergerstr. 10

D-82211 Herrsching

Tel: +49 8152 375-139

Fax: +49 8152 375-111

emtomic@hamamatsu.de

www.hamamatsu.com

PromoCell

PromoFectin facilitates the highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells.

PromoCell

It is a stable non-liposomal polymer, that is water-soluble and free of any components of animal origin. PromoFectin shows very low toxicity and is therefore ideally suited for the transfection of sensitive cells, yielding optimal transfection efficiencies and gene expression. Transfection with PromoFectin requires no medium change and it can be used for adherent and non-adherent cells. It compacts and protects the nucleic acid of interest, enables efficient transport into cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor delivery to the nucleus.

Special cell type specific PromoFectin variants are also available, which were developed for the efficient but gentle transfection of cells such as endothelial cells (e.g. primary HUVECs), hepatocytes, macrophages, or neuronal cells.

PromoKine also offers ready-to-use kits for fast and convenient detection and quantification of reporter gene expression including the commonly used reporter proteins luciferase (Firefly & Renilla), beta-galactosidase (reporter assay & staining kits) and GFP. The kits are highly sensitive, easy to handle and yield reproducible results. Substrates for the luciferase and beta-galactosidase assays are also available separately.

In addition, PromoKine provides a range of small-sized reporter plasmids (beta-galactosidase, luciferase, GFP, CAT, SEAP) as well as fusion plasmids, which allow high-level expression of a target gene of interest with a GFP or luciferase fusion tag at its C- or N-terminus.

See also our current promotion at www.promokine.info/promotion!


More Information:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

info@promokine.info

www.promokine.info

Beckman Coulter

Automated RNA Sequencing Service from Beckman Coulter Genomics

Provides Fast, High Quality Results.

Beckman Coulter

HiSeq* and Biomek platforms support a complete RNA-Seq solution  

High throughput end-to-end RNA-Seq Service from Beckman Coulter Genomics includes customized project design, automated Illumina* TruSeq* RNA-Seq library construction and flexible, multiplexed sequencing on the Illumina HiSeq platform.  Downstream bioinformatics analysis options include primary data analysis, differential gene expression, splice variant analysis, gene fusion identification and rare transcript identification. 

The new automated library construction process uses Illumina TruSeq RNA sample preparation kits in conjunction with Beckman Coulter Life Sciences’ Biomek* liquid handling platforms. Libraries show consistent size distribution and sequencing data is reproducible, showing good correlation with the published industry standard.

Total RNA from multiple species – including mammalian, plant and yeast samples – has been validated using the automated process.  The scalable, high throughput service allows processing from one to hundreds of samples without compromising quality. 

“Automated processing of RNA-Seq using the Biomek platform provides high quality data, and accommodates projects from small to large,” said Bernhard Spiess, vice president of sales and marketing, Beckman Coulter Genomics.  “We are very pleased with this enhanced service and the ability to provide scientists with reliable, reproducible results in a timely manner.” 

Technical experts in the company’s genomics services group utilize extensive experience to provide highly reliable data.  Typical turnaround time is four to seven weeks after sample receipt. For more information about this service, please visit www.beckmangenomics.com/rna-seq


* All trademarks are property of their respective owners.

Beckman Coulter, the stylized logo and Biomek are registered trademarks of Beckman Coulter, Inc.


Contact Information:

Hillary Hunter

Beckman Coulter Life Sciences Public Relations

Tel: +1 714 961 5620

hillarythunter@beckman.com

www.beckmancoulter.com

Lee Products

Miniature component specialists LEE PRODUCTS have recently published a new EFS (Electro Fluidic Systems) technical handbook which provides a valuable resource of product information along with practical engineering reference material.

Lee Products

Lee Product’s three Electro-Fluidic Systems groups produce high quality miniature solenoid valves, micro-dispense valves, atomising and dispense nozzles, fixed and variable volume pumps, integrated fluidic manifolds, inert tubing and fluid control components and custom engineered designs.  These products are used in medical and scientific instrumentation, analytical / clinical chemistry, in-vitro diagnostics, drug discovery and ink jet printing applications.

This new 323 page EFS handbook includes detailed information on Lee’s wide range of solenoid valves and pumps along with their manifolds, tubing, accessories, and special products, plus useful engineering and general information.


More details and copies of the handbook are available from:

Mike Stott

Lee Products Ltd

3 High Street

Chalfont St. Peter

Gerrards Cross

Bucks, SL9 9QE

Tel: +44 (0) 1753 886664

Fax:+44 (0)1753 889588

sales@leeproducts.co.uk

www.leeproducts.co.uk

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