Ready-to-use Angiogenesis Cell Model Systems
PromoCell provides four different ready-to-use angiogenesis cell model systems. Each of them allows the simulation of angiogenesis processes, by detailed in vitro recapitulation of the biological events involved in de novo vessel formation and remodelling: proteolytic activity, migration, proliferation and tube formation.
The PromoCell 3D Angiogenesis Kit examines all process together. It contains a collagen matrix with embedded endothelial cell spheroids and arrives ready-to-use in your lab. The only thing you need to do in order to start the assay is to add your test substance. Following proper stimulation de novo vessel formation occurs within two days, visible by sprouting of tubes into the surrounding matrix. The number and length of the sprouts corresponds to the pro- or anti-angiogenic potency of the test substance. Evaluation of the results is straightforward and easy, and staining procedures are not necessary.
To investigate the separate events of angiogenesis in a simulated in vitro environment, the PromoCell Transmigration Kit, the PromoCell Invasion Kit and the PromoCell Planar Migration Assay are useful platforms.
The PromoCell Transmigration Kit takes advantage of the ability of endothelial cells to migrate along cytokine gradients in a concentration-dependent manner, allowing for the measurement of the chemotactic potential of a test substance.
The PromoCell Invasion Kit is suitable for in vitro simulation of the proteolytic activity of endothelial cells as well as investigating directed migration. Hence, the impact of test substances on one or both of the aforementioned parameters can be easily monitored.
The PromoCell Planar Migration Assay is a 2D test platform for rapid evaluation of the influence of test substances on the migratory response of endothelial cells.