PromoFectin - Highly efficient transfection of primary cells and cell lines
PromoFectin facilitates the highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells.
It is a stable non-liposomal polymer, that is water-soluble and free of any components of animal origin. PromoFectin shows very low toxicity and is therefore ideally suited for the transfection of sensitive cells, yielding optimal transfection efficiencies and gene expression. Transfection with PromoFectin requires no medium change and it can be used for adherent and non-adherent cells. It compacts and protects the nucleic acid of interest, enables efficient transport into cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor delivery to the nucleus.
Special cell type specific PromoFectin variants are also available, which were developed for the efficient but gentle transfection of cells such as endothelial cells (e.g. primary HUVECs), hepatocytes, macrophages, or neuronal cells.
PromoKine also offers ready-to-use kits for fast and convenient detection and quantification of reporter gene expression including the commonly used reporter proteins luciferase (Firefly & Renilla), beta-galactosidase (reporter assay & staining kits) and GFP. The kits are highly sensitive, easy to handle and yield reproducible results. Substrates for the luciferase and beta-galactosidase assays are also available separately.
In addition, PromoKine provides a range of small-sized reporter plasmids (beta-galactosidase, luciferase, GFP, CAT, SEAP) as well as fusion plasmids, which allow high-level expression of a target gene of interest with a GFP or luciferase fusion tag at its C- or N-terminus.
See also our current promotion at www.promokine.info/promotion!