Product of the Week

PromoFectin - Highly efficient transfection of primary cells and cell lines
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PromoCell

PromoFectin - Highly efficient transfection of primary cells and cell lines

PromoFectin facilitates the highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells.

It is a stable non-liposomal polymer, that is water-soluble and free of any components of animal origin. PromoFectin shows very low toxicity and is therefore ideally suited for the transfection of sensitive cells, yielding optimal transfection efficiencies and gene expression. Transfection with PromoFectin requires no medium change and it can be used for adherent and non-adherent cells. It compacts and protects the nucleic acid of interest, enables efficient transport into cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor delivery to the nucleus.

Special cell type specific PromoFectin variants are also available, which were developed for the efficient but gentle transfection of cells such as endothelial cells (e.g. primary HUVECs), hepatocytes, macrophages, or neuronal cells.

PromoKine also offers ready-to-use kits for fast and convenient detection and quantification of reporter gene expression including the commonly used reporter proteins luciferase (Firefly & Renilla), beta-galactosidase (reporter assay & staining kits) and GFP. The kits are highly sensitive, easy to handle and yield reproducible results. Substrates for the luciferase and beta-galactosidase assays are also available separately.

In addition, PromoKine provides a range of small-sized reporter plasmids (beta-galactosidase, luciferase, GFP, CAT, SEAP) as well as fusion plasmids, which allow high-level expression of a target gene of interest with a GFP or luciferase fusion tag at its C- or N-terminus.

See also our current promotion at www.promokine.info/promotion!


More Information:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

info@promokine.info

www.promokine.info

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Solentim

Solentim has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development.

Solentim

Solentim, the developer of tools for shortening the upstream cell line development workflow, has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development. The new product can be used to quickly and reliably screen hundreds of plates from limited dilutions via automated image capture and analysis. By offering unmatched speed, Cell Metric™ rapid is ideal for large-scale projects investigating the optimal conditions for cell growth, processing many thousands of clones per day with walk-away ease of use. It will be launched and displayed at the 2nd Annual Cell Culture and Bioprocessing Conference in London (4-5 November), while more details can also be found on the Solentim website.

Using Cell Metric™ rapid, images from an entire 96-well plate can be captured in just 45 seconds, offering a more than three-fold improvement over the standard Cell Metric™. Clear, high resolution images are captured right up to the well edge to generate accurate, comprehensive and reproducible results. This makes it ideal for a wide range of analyses including confluence determination, clone selection and colony growth formation. The Cell Metric™ rapid can also integrate with Solentim’s well-proven, temperature-controlled microplate stacker and loader. This space-saving system holds a batch of 10 plates and has been designed to increase throughput as part of a workflow that reduces user intervention, maximising the use of resources and time. To achieve the highest levels of automation, the Cell Metric™ rapid can integrate with a wide variety of third-party robot arms, to form a completely automated robotic work cell.

Cell Metric™ rapid joins a growing product portfolio that offers market-leading clone imaging with significantly better optics and automated detection capabilities than previous generation products.  Existing users have reported shaving up to three months off the development of a new cell line when using the Cell Metric™, while the accuracy provided by the system’s high resolution, whole-well imaging maximises the number of single cells that can be positively identified as clonal.

Aaron Figg, Managing Director of Solentim commented, “Automated analysis systems have the potential to significantly improve commercial cell line development at every stage of the process, from calibration of the cell dispensing methods and liquid handling steps, to the clone selection and right through to the optimisation of growth conditions and scale-up ready for manufacture. Our customers continue to report significant improvements in their process quality, time and cost savings from incorporating Cell Metric™ into their workflows.”

For further details about Cell Metric™ rapid, visit the Solentim website.


Contact details:

Commercial Enquiries:

Solentim Ltd.
Dr. Ian Taylor
Sales
Tel.: +44 (0) 7554 446 185

ian.taylor@solentim.com
www.solentim.com


Media Enquiries:

Chempetitive Group (UK office)

Dr Nicole Liska
Science Writer
Tel.: +44 (0) 1223 828 205

nliska@chempetitive.com
www.chempetitive.com

Pall Life Sciences

96-well filter plates pre-filled with a family of chromatography sorbents for sorbent screening, or to optimize conditions in high throughput mode for protein purification.

Pall Life Sciences

AcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with Pall chromatography sorbents, to enable early chromatography sorbent screening for protein purification. Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption. Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using Pall 1 mL or 5 mL PRC prepacked columns filled with the same sorbents. 

AcroPrep ScreenExpert plate packages offer:

Flexibility: One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents or two optimization plates: one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent - You select your preference.
Easy Scale Up: Perform Design of Experiments (DoE) in 96-well plates and transfer to column chromatography. 1 mL or 5 mL PRC pre-packed columns filled with relevant sorbents allow convenient verification and small preparative work.
Minimized Sample Consumption: 50 μL of sorbent per well, allowing small sample usage. 1 mL well volume and 100 to 500 μL working volume.
Convenience: A disposable format in a convenient individual package. Compatible with manual multi-well plate vacuum manifold or robotic workstation-assisted operations.


Click here to learn more.

Eppendorf

The smart design of the Eppendorf DASbox makes the 4-fold units ideal for parallel processing of 4, 8, 12 or more bioreactors.

Eppendorf

The smart design of the Eppendorf DASbox makes the 4-fold units ideal for parallel processing of 4, 8, 12 or more bioreactors. Together with the Eppendorf DASware® software solutions the DASbox supports process development following the Quality by Design (QbD) approach by providing comprehensive information management, integration of 3rd party analyzers, and Design of experiments (DoE) tools as well as remote access via PC, notebook, iPhone® and iPad®.

The DASbox requires only 7.5 cm (3 in) of bench space per bioreactor maximizing use of lab space while being expandable in 4-fold unit increments to increase capacity as needed. Small working volumes of 60 - 250 mL make it a perfect fit for clone/cell line screening, media optimization and small scale process development.

Suitable for microbiology and cell culture, the DASbox features advanced process control and precise monitoring of all critical process parameters.

  • Integrated Feeding and Monitoring: Variable speed pumps and standard pH and DO sensors (redox or level options and optical pH sensors available)
  • Liquid-free temperature control for easy handling
  • TMFC Gassing: Integrated mass flow-controlled gas mixing system for continuous mixing of Air, N2, O2 and CO2
  • LC Color Display: All vessels and parameters at a glance


Contact:

Eppendorf AG Bioprocess Center

Rudolf-Schulten-Str. 5

52428 Juelich

Germany

Tel. + 49 (0) 2461 980 400

Fax + 49 (0) 2461 980 499

E-Mail: bioprocess-info@eppendorf.de

Internet: www.eppendorf.com

Eppendorf

The new BioBLU 1 vessels fill the gap between the BioBLU 0.3 mini-scale bioreactors and the larger BioBLU 5c.

Eppendorf

The Eppendorf BioBLU family of rigid-walled stirred-tank single-use vessels is growing: The new BioBLU 1 vessels fill the gap between the BioBLU 0.3 mini-scale bioreactors and the larger BioBLU 5c. Available for cell culture (320 mL - 1.25 L working volume) and microbial applications (250 mL - 1.25 L) they perfectly add to the Eppendorf portfolio of scalable single-use solutions. Scientists working in cell culture and microbial process development benefit from BioBLU 1 through easy handling, minimized set-up time and the elimination of cleaning and sterilization steps. Parallel processing of 4, 8 or more vessels using DASGIP Control software shortens development times and reduces costs.

  • Parallel temperature control with the Eppendorf DASGIP® Bioblock (adaptor kits available)
  • Proven cell culture single-use design with pitched blade impellers
  • Fermentation vessel featuring Rushton-type impellers and innovative baffles for cooling
  • Liquid-free exhaust condensation with Peltier technology


Contact:

Eppendorf AG Bioprocess Center

Rudolf-Schulten-Str. 5

52428 Juelich

Germany

Tel. + 49 (0) 2461 980 400

Fax + 49 (0) 2461 980 499

E-Mail: bioprocess-info@eppendorf.de

Internet: www.eppendorf.com

PeproTech

PeproGrow-1 is designed to be used as a supplement to serum-free cell culture media formulations.

PeproTech

PeproGrow-1 (Catalog # 700-C100) is designed to be used as a supplement to serum-free cell culture media formulations that can sustain the growth of adherent mammalian cell lines, and has been tested with HEK-293, HeLa, and A549 cells. This kit could potentially improve the culturing conditions of other adherent cells, but suitability for cells other than those pre-tested is not guaranteed. PeproGrow-1 is protein-free, has a chemically defined composition, and does not contain any animal-derived materials. It is intended specifically to be used with DMEM/F12 basal media (Invitrogen catalog # 10565*) and contains enough material to supplement 10L of media.


Contact:

Dr. Peter Bubeck, Produktmanager

PeproTech GmbH

Oberaltenallee 8

22081 Hamburg


Tel.:  040/73435-7770

Fax.: 040/73435-7779

info@peprotech.de

www.peprotech.de

PromoCell

The PromoCell MSC Growth Medium DXF eliminates the risks of using traditional FCS-containing MSC media and this innovative formulation provides significant benefits.

PromoCell

Chemically defined and humanized culture of MSC


Undifferentiated Mesenchymal Stem Cells (MSC) can be expanded in vitro to produce of large numbers of cells. For this expansion, traditional MSC culture media that contains special pre-qualified lots of fetal calf serum has been necessary. In addition to the serious ethical concerns relating to the use of FCS, this ill-defined component of animal origin is the major obstacle in providing consistent culture conditions and optimal performance of MSC.


The PromoCell MSC Growth Medium DXF eliminates the risks of using traditional FCS-containing MSC media. This innovative formulation provides significant benefits:

·  Chemically defined

·  Humanized: animal-free / xeno-free

·  No adaption of cells required

·  Outstanding expansion performance

·  Maintenance of multipotency

·  Supports primary isolation

·  Standardized: minimal lot-to-lot variation

·  Cost effective: more cells with less medium


For economical serum-free routine culture of MSC, the MSC Growth Medium DXF may be used in combination with bovine fibronectin as an ECM coating and our Detach Kit. For chemically defined culture conditions, the trypsin-containing DetachKit should be replaced by the Cell Dissociation Solution ACF. The use of human fibronectin and the Cell Dissociation Solution ACF completes the MSC Growth Medium DXF to yield a powerful animal-free and chemically defined complete culture system.

See also our current promotion on MSC Growth Media: www.promocell.com/promotion


More Information:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg
Germany

info@promocell.com

www.promocell.com

Tosoh Bioscience

The packing materials and packed columns are an ideal choice for high performance liquid chromatography.

Tosoh Bioscience


Extensively used in laboratories all over the world, our TSKgel is designed for researchers seeking the highest level of performance. Covering the total range of HPLC, this product is made from small, spherical particles and offers high resolution (even at high flow rates), excellent reproducibility, and long column life. Scaling up from analytical to preparative columns is made simple and easy.


Contact:

Tosoh Bioscience GmbH

Zettachring 6

70567 Stuttgart

Germany

Tel: +49-711-132570

Fax: +49-711-1325789

info.tbg@tosoh.com

www.tosohbioscience.com 

Advanced Analytical Technologies

The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).

Advanced Analytical Technologies

By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.

This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.

Currently kits are available to:

• quantify and qualify NGS fragments.
• quantify and qualify RNA.
• quantify and qualify genomic DNA.
• do Mutation Detection, also known as TILLiNG, a reverse genetics technique.

The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.

Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.

An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.

Contact:

Advanced Analytical Technologies GmbH

Lutz Büchner

Im Neuenheimer Feld 583

69120 Heidelberg

Tel.: +49-(0)-6221-868058-20

lbuechner@aati-de.com

www.aati-de.com 

ForteBio - a division of Pall Life Sciences

ForteBio announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems.

ForteBio - a division of Pall Life Sciences

ForteBio, a division of Pall Life Sciences and a leading supplier of instrumentation for accelerated drug discovery and development, today announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems. The Octet HTX system addresses the acute industry need for increased analysis speed and sample throughput when characterizing large numbers of therapeutic candidates.

Octet systems provide unique insights into drug-target binding interactions, insights that are used to fine-tune the affinity and specificity of drug molecules. The Octet HTX instrument performs kinetic analysis of up to 96 binding interactions simultaneously, a capability offered by no other stand-alone label-free platform. The system offers dramatic time savings for researchers studying large panels of drug candidates, and provides innovative data analysis tools for developers of monoclonal antibodies and other biotherapeutic entities. In addition, the Octet HTX performs full 96-well plate determinations of protein concentrations in as little as 2 minutes.

“The need for higher analytical throughput is a central theme with our pharmaceutical and biopharm customers,” noted Robert Wicke, General Manager at Pall’s ForteBio Division. “We believe researchers will be delighted by the Octet HTX system. The HTX system was engineered for speed while maintaining peak performance, and will directly address critical bottlenecks in the drug development process.”

The Octet HTX system was unveiled today at the BioProcess International 2013 conference in Boston, Mass.

ForteBio
Gemma Milan

Phone 1-650-289-6800
email: Gemma_milan@pall.com

www.fortebio.com

ProteinSimple

Gel-free. Blot-free. Hands-free. A Simple Western is the Western you know well, only simplified.

ProteinSimple

Gel-free. Blot-free. Hands-free.

A Simple Western is the Western you know well, only simplified. It is not a workaround to the existing process, a Western-like substitute, or a partial solution. It's the real thing, completely reinvented. We at ProteinSimple think that's something to get excited about!

Get your time back. Load your samples in Simon, Sally or Peggy, press start and go enjoy your new hands-free time. Come back a few hours later to fully analyzed results.
Get more consistency. People introduce variability—we arehuman after all. Simple Westerns are fully automated, so they just give you more reproducible data.
Get truly quantitative. Since blot transfer of proteins isn't needed, you can get straight to real, quantitative results.
Get more efficient. Sometimes you can only squeeze out enough sample for one experiment. Simple Westerns require a lot less protein and antibody so you have enough for those replicates.


• Learn about Simple Westerns
• Learn about Peggy
• Learn about Sally
• Learn about Simon
• Learn about NanoPro 1000
• Learn about Compass Software 21 CFR Part 11 Compliance
• Simple Western Systems Comparison Chart


Contact:

Dr. Markus Goddemeier

Sales Specialist

T: +49 (0)761 477 07 63

M: +49 (0)162 985 79 53

www.proteinsimple.com

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No liability assumed, Date: 23.04.2014

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