SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. SuperScript® III RT features:
Thermostability—half-life of 220 min at 50°C
Yield—reduced RNase H activity for more full-length cDNA
Specificity—full activity at 50°C for increased specificity with gene-specific primers
Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3´ and 5´ RACE
At this year’s Achema, Eppendorf and the DASGIP Information and Process Technology GmbH presented for the first time the new DASbox single-use vessel for parallel bioprocessing.
The development of the DASbox single-use vessel reinforces the strong synergy that benefits both DASGIP and the Eppendorf AG, which acquired the bioprocessing specialist this past January. “Within a very short time we were able to combine the long-standing competency of the Eppendorf AG in the field of plastics technology with our bioreactor expertise, which led to a new product”, explains Dr. Matthias Arnold, member of DASGIP management and head of the development department.
The DASbox single-use vessel is the first single-use bioreactor which was developed for the DASGIP Parallel Bioreactor Systems. As a fully instrumented mini bioreactor with a working volume of 60-250 ml, it was constructed specifically for use in combination with the DASGIP DASbox mini bioreactor system. Especially with cell culture users in mind, the advantages of single-use technology are combined with the benefits of parallel cultivation and the full functionality of industrial bioreactors. All critical process parameters such as pH, soluble oxygen and optical density can be monitored and controlled using industry standard probes.
Integrated dip tubes allow for controlled addition of liquids, sampling, as well as fully massflow controlled gas supply. The magnet coupled stirrer is only one of the examples for the uncompromising sterile technology of this single-use bioreactor. A special feature of the DASbox single-use vessel is the novel, liquid-free Peltier element temperature control and condensation. This innovation, which enables, amongst others, efficient liquid condensation from the exhaust air, represents a further milestone in the transfer of the requirements of classic bioreactor technology to single-use technology.
The DASbox single-use vessel accelerates bioprocess development. The small working volumes save precious cell material and media, and extensive cleaning and sterilization procedures are superfluous. Less time is required for installation of the bioreactor, and cross contaminations are virtually excluded. Parallel cultivation under precisely controlled conditions delivers reliable results, fast. Further, the comprehensive software functions of DASGIP DASware allow user friendly and detailed capture, saving, analysis and management of resulting data.
Automated RNA Sequencing Service from Beckman Coulter Genomics
Provides Fast, High Quality Results.
HiSeq* and Biomek platforms support a complete RNA-Seq solution
High throughput end-to-end RNA-Seq Service from Beckman Coulter Genomics includes customized project design, automated Illumina* TruSeq* RNA-Seq library construction and flexible, multiplexed sequencing on the Illumina HiSeq platform. Downstream bioinformatics analysis options include primary data analysis, differential gene expression, splice variant analysis, gene fusion identiﬁcation and rare transcript identiﬁcation.
The new automated library construction process uses Illumina TruSeq RNA sample preparation kits in conjunction with Beckman Coulter Life Sciences’ Biomek* liquid handling platforms. Libraries show consistent size distribution and sequencing data is reproducible, showing good correlation with the published industry standard.
Total RNA from multiple species – including mammalian, plant and yeast samples – has been validated using the automated process. The scalable, high throughput service allows processing from one to hundreds of samples without compromising quality.
“Automated processing of RNA-Seq using the Biomek platform provides high quality data, and accommodates projects from small to large,” said Bernhard Spiess, vice president of sales and marketing, Beckman Coulter Genomics. “We are very pleased with this enhanced service and the ability to provide scientists with reliable, reproducible results in a timely manner.”
Technical experts in the company’s genomics services group utilize extensive experience to provide highly reliable data. Typical turnaround time is four to seven weeks after sample receipt. For more information about this service, please visit www.beckmangenomics.com/rna-seq
* All trademarks are property of their respective owners.
Beckman Coulter, the stylized logo and Biomek are registered trademarks of Beckman Coulter, Inc.
Sartorius Stedim Biotech presents the new system FlexAct® that enables you to custom- configure single-use solutions for entire biomanufacturing steps.
FlexAct® is a new system that enables you to custom- configure single-use solutions for entire biomanufacturing steps. The FlexAct® system consists of the central opera- ting module that offers the widest variety of configuration options so you can take complete control of practically any steps in upstream and downstream processing.
Sartorius Stedim Biotech presents the FlexAct® BP for buffer preparation. Next in line are media preparation (MP), cell harvest (CH), UF | DF crossflow (UD), virus removal (Vr), virus inactivation (VI), polishing (PO), form | fill (FF) and form | transfer (FT).
The core of FlexAct®, the central operating module (COM), has been designed to be used in any application. This means you can quickly reconfigure FlexAct® in just a few easy steps. For example, take the FlexAct® BP for buffer preparation and effortlessly convert it into FlexAct® VR for virus removal.
And it goes without saying that the technologies and products used in FlexAct® are scalable. This makes FlexAct® a future-proof system in every respect; one that gives you the greatest possible flexibility.
PromoKine’s plant-derived cytokines and growth factors are free of animal components, serum traces, and antibiotics.
Growing importance of fields like stem cell research require cytokines and growth factors produced in a safe host organism that prevent the occurrence of unwanted cellular responses caused by high endotoxin levels and other bacterial or mammalian impurities. Many commercially available cytokines and growth factors have endotoxin levels and other impurities high enough to seriously affect cellular processes like proliferation or differentiation.
PromoKine’s plant-derived cytokines and growth factors are free of animal components, serum traces, and antibiotics. They are also free of human, animal or bacterial infectious agents and have an endotoxin content typically 20-times lower than proteins produced in E. coli. Thus, they avoid problems caused by too high endotoxin levels and improve critical applications, e.g. in stem cell research.
ForteBio announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems.
ForteBio, a division of Pall Life Sciences and a leading supplier of instrumentation for accelerated drug discovery and development, today announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems. The Octet HTX system addresses the acute industry need for increased analysis speed and sample throughput when characterizing large numbers of therapeutic candidates.
Octet systems provide unique insights into drug-target binding interactions, insights that are used to fine-tune the affinity and specificity of drug molecules. The Octet HTX instrument performs kinetic analysis of up to 96 binding interactions simultaneously, a capability offered by no other stand-alone label-free platform. The system offers dramatic time savings for researchers studying large panels of drug candidates, and provides innovative data analysis tools for developers of monoclonal antibodies and other biotherapeutic entities. In addition, the Octet HTX performs full 96-well plate determinations of protein concentrations in as little as 2 minutes.
“The need for higher analytical throughput is a central theme with our pharmaceutical and biopharm customers,” noted Robert Wicke, General Manager at Pall’s ForteBio Division. “We believe researchers will be delighted by the Octet HTX system. The HTX system was engineered for speed while maintaining peak performance, and will directly address critical bottlenecks in the drug development process.”
The Octet HTX system was unveiled today at the BioProcess International 2013 conference in Boston, Mass.
The ProdX software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient.
Seamless Integration of Product Inspection Devices
Whatever your role in product inspection management, you will have plenty of challenges to face in marketplaces that increasingly demand more for less.
ProdX Inspect data management software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient. ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
ProdX Savings Calculator
Calculate your time savings potential with ProdX
This Calculator runs through all costs involved in the setup and data collection processes. It calculates the amount you could save per year by comparing the time requirement of manual processes and the automated data collection process this software offers.
ProdX Quality Management & AssuranceThe innovative ProdX software automatically collects the critical inspection process data you need in one convenient location significantly reducing the need to interact directly with the Critical Control Points on your factory floor. This saves time and effort enabling quality operatives to focus on other issues.
ProdX Production Process Managment
ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
PromoKine is now adding more than 200 new ELISA kits for target molecules which play integral roles in cell signaling research.
PromoKine currently offers ~400 ELISA kits for detecting and quantifying the "free" forms of human, mouse, rat, monkey and pig cytokines, growth factors and other cell biology target molecules in cell culture supernatants, plasma, serum and other body fluids. Some can even be used with tissue lysates! Our ready-to-use ELISA kits are very convenient, including a user-friendly and easy-to-follow protocol along with all the necessary components to perform a highly sensitive, reliable and reproducible ELISA.
In addition, we also provide cost-effective ELISA Development Kits containing all required reagents to assay your target protein/peptide in ten 96-well microtiter plates (approximately 1,000 assays per kit).
PromoKine is now adding more than 200 new ELISA kits for target molecules which play integral roles in cell signaling research; such as diverse interleukins, FGFs, CD markers, BMPs, MIPs, MMPs and MCPs as well as Activin A, beta-NGF, Cardiotrophin, Lymphotactin, Oncostatin M, Insulin and several other hormones. We also have launched a new line of Cell-based Phosphorylation ELISAs.
Along with this, we are extending our comprehensive product line of recombinant cytokines, growth factors, soluble receptors, hormones and other proteins/peptides involved in cell signaling as well as our wide range of antibodies against these molecules.
The Biometra GmbH, a company of the Analytik Jena AG, now offers illuminators with high-performance blue light LEDs for gel documentation purposes.
Blue light transilluminators are a quite interesting alternative to UV tables as there is no risk of sample damage during illumination. This is important when samples shall be processed furthermore after gel documentation. Users also benefit from it as there is no risk of UV exposure. Blue light excitation is applicable for fluorescent dyes for nucleic acid or protein stains with excitation wavelength around 470 nm. Examples for compatible stains are: SYBR® Green, GelGreenTM, SYBR® Safe, SYBR® Gold or SYPRO® Ruby. The amber lid of the instrument allows the direct view to the fluorescent samples. There is no need for separate glasses. The new blue light illuminators are available in two versions: "BLstar 9", the handy solution for mini gels and "BLstar 16" for gels up to size of 16 cm x 20 cm.
Thermo Fisher Scientific releases new small benchtop centrifuge, which provides scientists with outstanding capacity in a compact design.
Thermo Fisher Scientific Inc. has launched a new small benchtop centrifuge to meet clinical and research needs alike.
“Centrifuges are in constant use and demand so preventing downtime and bottlenecks is crucial,” said Maurizio Merli, product director, Thermo Fisher Scientific. “With features designed for simplicity, safety and flexibility, the new small benchtop centrifuges can help scientists save time and maximize resources.”
The new centrifuges provide:
Application flexibility, convenient cleaning and ease-of-use with Thermo ScientificAuto-Lock rotor exchange
Versatility to spin blood tubes, conical tubes, microtubes and microplate compatibility with sealing options
One-handed sample protection with Thermo Scientific ClickSeal biocontainment lids, certified by the Public Health Laboratory Service in Porton Down
Intuitive glove and detergent-friendly interface with a bright display, one-touch operation with pre-set protocol options and optional password protection
Efficient testing of the pro- or anti-angiogenic capacity of substances and drugs demands for standardized and easy-to-use test systems that deliver reproducible data quickly.
PromoCell provides four different ready-to-use angiogenesis cell model systems. Each of them allows the simulation of angiogenesis processes, by detailed in vitro recapitulation of the biological events involved in de novo vessel formation and remodelling: proteolytic activity, migration, proliferation and tube formation.
The PromoCell 3D Angiogenesis Kit examines all process together. It contains a collagen matrix with embedded endothelial cell spheroids and arrives ready-to-use in your lab. The only thing you need to do in order to start the assay is to add your test substance. Following proper stimulation de novo vessel formation occurs within two days, visible by sprouting of tubes into the surrounding matrix. The number and length of the sprouts corresponds to the pro- or anti-angiogenic potency of the test substance. Evaluation of the results is straightforward and easy, and staining procedures are not necessary.
To investigate the separate events of angiogenesis in a simulated in vitro environment, the PromoCell Transmigration Kit, the PromoCell Invasion Kit and the PromoCell Planar Migration Assay are useful platforms.
The PromoCell Transmigration Kit takes advantage of the ability of endothelial cells to migrate along cytokine gradients in a concentration-dependent manner, allowing for the measurement of the chemotactic potential of a test substance.
The PromoCell Invasion Kit is suitable for in vitro simulation of the proteolytic activity of endothelial cells as well as investigating directed migration. Hence, the impact of test substances on one or both of the aforementioned parameters can be easily monitored.
The PromoCell Planar Migration Assay is a 2D test platform for rapid evaluation of the influence of test substances on the migratory response of endothelial cells.
LentiKat’s offers an unusual investment opportunity
LentiKat’s owns, manufactures and distributes a patented immobilization into lentil-shaped Lentikats Biocatalysts. The unique lentil-like shape of the Lentikats Biocatalyst ensures unlimited diffusion of substrate(s) into any part of the matrix, while still retaining a convenient size for easy separation from the reaction medium. They find application in the Pharma & Food industry (including bio-based chemicals), Wastewater treatment as well as Biofuels. more...
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. more