Magnet Assisted Transfection (MATra)
Magnet Assisted Transfection (MATra) is an easy-to-handle, very fast (15 minutes!) and highly efficient technology to transfect cells in culture. With MATra, all types of nucleic acids from plasmid DNA or siRNA to oligonucleotides can be delivered – even into hard-to-transfect cell types such as neuronal or stem cells. Using this new technique, nucleic acids are in a first associated with specific magnetic nanoparticles. A magnetic force is applied then and the nucleic acids will be quantitatively and quickly drawn towards and delivered into the target cells leading to efficient transfection without disturbing the membrane architecture and without causing chromosomal damage. MATra can be used with adherent or suspension cells and is functional with or without serum. Compared to other transfection techniques (e.g. electroporation or lipofection), MATra results in extremely low cytotoxicity and is very cost-effective. MATra can also be adapted to high-throughput transfection assays using robotic stations and adapted protocols.
Our PromoFectin Transfection Reagent provides highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells. It consists of a non-liposomal polymer that is stable, water-soluble and free of any components of animal origin. PromoFectin compacts and protects the nucleic acid of interest, enables efficient transport into the cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor the delivery to the nucleus. Transfection with PromoFectin results in very low toxicity and is therefore ideally suited for the transfection of very sensitive cells, yielding optimal effects on gene expression. It can be used for adherent and non-adherent cells and the protocol does not require a medium change. Special cell type-specific PromoFectin variants are also available, which have been developed for an optimized transfection of e.g. endothelial cells (HUVECs, HDMECs etc.), hepatocytes, macrophages, dendritic or neuronal cells.
In addition, PromoKine provides a range of small-sized reporter plasmids (beta-galactosidase, luciferase, GFP, CAT, and SEAP) as well as fusion plasmids, which allow high-level expression of a target gene of interest with a GFP or luciferase fusion tag at its C- or N-terminus. The corresponding reporter assays (luciferase, beta-galactosidase & GFP) are also available.
See our current MATra and PromoFectin promotions at www.promokine.info/promotion!
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