Product of the Week

Eppendorf BioFlo 320 Universal Bioprocess Control Station
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EPPENDORF

Eppendorf BioFlo 320 Universal Bioprocess Control Station

A robust industrial design, intelligent sensors, Ethernet connectivity, and enhanced software capabilities are only a few of the features that set it apart from the competition. Combined with a sincere commitment to quality, the BioFlo 320 truly is the premium choice in bench-scale bioprocess control stations.

Flexible

  • Autoclavable vessels and comprehensive portfolio of BioBLU® Single-use Vessels provide process customization
  • Eppendorf exclusive packed-bed and cell lift impeller designs for continuous processes
  • Universal connections for analog or digital Mettler Toledo® ISM sensors reduce sensor complexity
  • Thermal mass flow controllers for sparge and overlay gas can be upgraded in the field

Powerful

  • Extensive working volume range of 250 mL – 40 L on a single control platform
  • High-powered direct and magnetic drive motor assemblies
  • Up to six integrated pumps capable of operating in variable speed mode
  • Industry standard Ethernet communication for multi-unit control of up to eight systems

Industrial

  • Industrial design featuring stainless steel front, back, and utility panels
  • Left- and right-handed orientations to maximize lab space efficiency
  • Hemispherical vessel nest design for minimum footprint

For more information please click here.

Contact

Eppendorf AG Bioprocess Center

Rudolf-Schulten-Str. 5

52428 Juelich

Germany

Tel: +49 2461 980-400

E-Mail: bioprocess-info[at]eppendorf.de

Internet: www.eppendorf.com

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Products

BD

With 100g samples, the Peptone Starter Paks allow for you to identify the correct supplements for your specific cell line faster.

BD Biosciences

BD is helping you rethink supplementation. For more than 100 years, leading pharmaceutical companies have trusted BD peptones and supplements to help improve cell culture performance with higher cell titers and yield.

The new BD Peptone Starter Paks provide samples of our most widely used peptones tailored for specific applications, including monoclonal antibodies, recombinant proteins, and vaccines, for use in both mammalian cell culture and microbial fermentation.

With 100g samples conveniently packed in one box, you can identify the correct supplements for your specific cell line faster.

Request a starter pak today and start maximizing your cell’s performance. Go to www.bdbiosciences.com/go/peptone.

Peptone Starter Paks are for evaluation use only.


Contact:

BD

7 Loveton Circle

Sparks, MD 21152

bdbiosciences.com/advbio

Analytik Jena

The BioShake series contains high-speed mixer and thermal mixer for small and very small volumes in microplates and reaction tubes and allows for the first time high precise and efficient mixing in the microliter scale for a wide range of applications.

Analytik Jena

Assays in microplates or reaction vessels can be realized fast and safe with using adjustable speed of 200 up to 3,000 rpm. The BioShake mixing-technology is obviously more robust, vibration free and needs less maintenance compared to classical mixers.

Programming the BioShake XP and BioShake iQ works via direct touch buttons. In addition two buttons for start and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.

The short mix button allows short and fast mixing in between.

The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time, mixing frequency and for BioShake iQ additional temperature.

The BioShake iQ is the high end thermal mixer of the BioShake series. In addition to the technical specifications of the model BioShake XP, the BioShake iQ comes with an very accurate heating technology. This allows highly reproducible results. The temperature range from RT to 99 °C is adjustable in 0.1 °C steps.

The temperature accuracy is ±0.1 °C, the temperature uniformity through all samples is better than ±1 °C.

The BioShake series is characterized by minor space requirement.

The BioShake series comes with a variety of standardized and specific adapter plates. The adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels. An excellent temperature uniformity and homogeneity is guaranteed by using these adapters.

Here you get more information.

Contact: 

Dana Schmidt

Tel: +49 36 41 77-92 81 

lifescience[at]analytik-jena.de

A Thermo Fisher Scientific Brand

The GeneArt® CRISPR Nuclease mRNA is wild type Cas9 as mRNA, for genome editing using CRISPR-Cas9 technology.

Life Technologies

High-efficiency CRISPR genome-editing tools for multiplex editing

The GeneArt® CRISPR Nuclease mRNA is wild type Cas9 as mRNA, for genome editing using CRISPR-Cas9 technology. Ready-to-transfect Cas9 mRNA circumvents the need for time-consuming cloning steps required when using CRISPR vector systems. Cas9 mRNA can be co-transfected with in vitro transcribed guide RNA (IVT gRNA) or a synthetic gRNA expression cassette containing U6 promoter. The gRNA expressing cassette and IVT gRNA template can be ordered as GeneArt® CRISPR Strings™ DNA, a 500 bp DNA fragment. Following transfection, the Cas9 protein is directed by gRNA to target specific genomic locus. This system allows multiplex genome editing, where multiple target gene sequences can be edited simultaneously in a single transfection reaction with the addition of multiple gRNAs. The system is versatile and simple to use, and changing target specificity only requires a change in the design of the GeneArt® CRISPR Strings™ DNA.

For further information please click here.

3PBiopharmaceuticals

The experience acquired with one of its clients allows 3P the capability to offer this alternative technology in its services pipeline.

3PBiopharmaceuticals

The Upstream and the Technical Services teams have headed a cell free process; this is a state of the art technology that allows obtaining a specific enzyme in a non-stop fermentation process. This is possible thanks to an enzymatic biochemical reaction without any cellular mechanism interaction. 

This is an important landmark for the company in several aspects: 

  1. The successful coverage of a continuous production processes.
  2. Control of temperature during all stages to ensure stability throughout the process with adaptation of equipments and facilities to guarantee this condition
  3. The scaling-up of this technology with the singularity of maintaining constantly the temperature to guarantee the reproducibility of the process.
  4. The development of this alternative technology opens the door to those potential clients who need this type of resources to produce proteins and small molecules and sets the company in a leading position in the global biotechnology industry.

About 3PBiopharmaceuticals. 

3P is a CDMO organization that develops and manufactures biological and cellular therapy products for preclinical, clinical trials and commercial phases for other companies under GMP conditions.

Contact Information:

Edurne Gil  

3P Biopharmaceuticals Communications Manager

Tel.: + 34 984 346480

eMail: egil[at]3pbio.com

Web: www.3pbio.com

METTLER TOLEDO

METTLER TOLEDO now offers a new family of high-precision weighing platforms that provide superior accuracy and reliability in harsh industrial conditions with flexibility to be used for many applications.

METTLER TOLEDO

The new PBK9 and PFK9 Weighing Platforms provide the rugged and reliable operation customers have come to expect from METTLER TOLEDO, paired with advanced technology for high-precision weighing.

Benefits of PBK9 and PFK9 Weighing Platforms include:

  • Outstanding accuracy: The platforms are ideally suited for all weighing processes in which quality is critical and tolerances need to be minimized.
  • Reliable performance: The platforms notify the operator when recalibration or adjustments are necessary due to environmental changes.
  • Easy maintenance: Automated calibration with a built-in calibration weight and plug-in cables allow for easy maintenance.
  • Versatile and Robust: The robust load cell with integrated overload protection and IP66/IP68 ingress protection makes the platforms suitable for a variety of challenging environments including wet areas, locations with heavy dust or workplaces that need regular cleaning.

The PBK9 and PFK9 Weighing Platforms virtually eliminate the impact of environmental interferences that cause errors and result in wasted ingredients or rejected batches. Advanced Monobloc and filter technology guarantees the highest precision and reliability possible.

In addition, the PBK9 and PFK9 weighing platforms are excellent standalone solutions and they also operate well as components in applications ranging from counting and formulating to filling. PBK9 and PFK9 Weighing Platforms offer multiple options, including mild, galvanized or stainless-steel for applications in wet, dusty, harsh or hazardous environments. Nine sizes and twelve capacities from 0.6 kilograms to 3 tons with resolutions up to 750,000 divisions respectively 30,000e (legal for trade) are available. All platforms can be connected to numerous METTLER TOLEDO terminals or directly connected to a control system.

For more detail visit: www.mt.com/PBK9


Media Contact:

Melissa Braithwaite

METTLER TOLEDO

Melissa.Braithwaite[at]mt.com

614-841-7348

CANDOR

ReadyTector is an easy, quick and clear all-in-one detection solution for Western blotting.

Candor Bioscience GmbH

All-in-one solution for Western blots

The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously. 

The only additional step is washing with the specific ReadyTector Wash Buffer.

ReadyTector for Western blotting is easy, quick and clear:

  • easy – very simple procedure
  • quick – very fast procedure with very quick results
  • clear – no background

The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.


Contact:

Candor Bioscience GmbH

Simoniusstrasse 39

88239 Wangen, Germany

Tel.: +49(0)-7522-7952-70

Fax: +49(0)-7522-7952-729

info[at]readytector.com

www.readytector.com

Life Technologies

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs.

Life Technologies

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. SuperScript® III RT features:

  • Thermostability—half-life of 220 min at 50°C
  • Yield—reduced RNase H activity for more full-length cDNA
  • Specificity—full activity at 50°C for increased specificity with gene-specific primers
  • Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3´ and 5´ RACE

For further information please click here.

TAP Biosystems

TAP Biosystems installs CellBase CT system at PrimeCell to provide multiple Autologous Cell Therapies.

TAP Biosystems

TAP Biosystems, a leading supplier of innovative cell culture systems and consumables for life science applications, is pleased to announce it is installing a system to automate the ex-vivo expansion of autologous cell therapies (CellBase CT™) at Czech-based firm PrimeCell, one of Central and Eastern Europe’s largest regenerative medicine companies.

The CellBase CT system at PrimeCell will be used in their new GMP facility at the National Tissue Centre Inc to rapidly scale up their manual cell culture processes in T-flasks and will provide a fully-contained aseptic cell expansion area. This will allow scientists to manufacture multiple autologous cell therapies from many individual patients in parallel and will enable the company to supply clients, including regional transplant centres in the Czech Republic, with a range of clean autologous therapies.

Dr Ralph von Strandmann, TAP Biosystems' European Sales Manager stated: “With EU funding for new technologies, there has been an increasing interest in manufacturing biotechnology products in Central and Eastern Europe. This has led progressive companies here to realise that utilising efficient methods of manufacturing cell therapies will provide them with a significant advantage in this competitive sector.”

Dr. von Strandmann added: “We are pleased that PrimeCell will be using CellBase CT to automate expansion of their autologous stem cell therapies.  It is an excellent, cost-efficient alternative to labour intensive manual cell culture and could help make autologous cell therapies less expensive and more widely accessible to patients in Central and Eastern Europe.”

Contact:

TAP Biosystems

Matthew Walker

Head of Communications

York Way, Royston

Hertfordshire, SG8 5WY, UK

Tel: +44 (0) 1763 227200

Fax: +44 (0) 1763-227201

matthew.walker[at]tapbiosystems.com

www.tapbiosystems.com

PROMEGA

The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR) Assay allows you to detect the activities of firefly and NanoLuc® luciferases in a single sample.

Promega Corporation

The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR) Assay allows you to detect the activities of firefly and NanoLuc® luciferases in a single sample. Improved firefly chemistry and the bright, sensitive NanoLuc® luciferase make this a highly sensitive dual assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.

In the Nano-Glo® Dual-Luciferase® Reporter Assay, firefly luciferase activity is measured first using the ONE-Glo™ EX Luciferase Assay Reagent. NanoDLR™ Stop & Glo® Reagent is then added to quench the firefly signal and provide the substrate needed to measure NanoLuc® luciferase activity. The signal half-life is greater than two hours for both reporters.

HEK293 cells transfected with a 1:1:8 ratio of either TK-Rluc (Renilla):TK-Fluc (firefly):carrier DNA or TK-Nluc (NanoLuc):TK-Fluc:carrier DNA and assayed with NanoDLR™, DLR™ or Dual-Glo® Dual-Luciferase Assay System as indicated.  When luciferases are expressed from the same promoter, the NanoDLR™ Assay provides bright signal for both Fluc and Nluc reporters with stable "glow-type" signals.

Potent inhibition of firefly luciferase (Fluc) provides excellent signal separation, allowing accurate measurement of even low amounts of NanoLuc® luciferase (Nluc), even in the presence of high Fluc activity.

Even extremely high Fluc signals are reduced to near background levels by NanoDLR™ Stop & Glo® Reagent, providing excellent signal separation and low variability without sacrificing the sensitivity of two bright luminescent readouts.

For mor information please click here.


Contact:

Promega GmbH

Dr. Anette Schwenzer

Schildkrötstr. 15

68199 Mannheim

Tel.: +49 621 8501-110

anette.schwenzer@promega.com

www.promega.com 

Biological Industries

Isolate, grow, maintain and differentiate human mesenchymal stem cells from various sources with a complete, optimized culture system.

Biological Industries

Biological Industries offers a full product portfolio, including stem cell culture media, freezing media, attachment solution, cell dissociation solutions and initial differentiation kits. 

Isolation and Culture

MSC NutriStem® XF line and its supporting reagents are optimized for human mesenchymal stem cells from a variety of tissue sources and promise optimal growth and maintenance of hMSC, high proliferation rate, superior maintenance of cell characteristics, fibroblast-like morphology maintenance and long-term growth

Differentiation

MSC GoTM Osteogenic, Adipogenic and Chondrogenic  XF series offer a complete system for the evaluation and  reliable induction of hMSC from various sources into adipocytes, chondrocytes and osteoblasts/osteocytes. The kits are designed to be user friendly with all the growth factors and supplements necessary for the direct differentiation of hMSC

All products are completely free of serum and non-human derived components, hence:

  • Eliminate contagious health risks from viral agents, mycoplasma, and prions
  • Eliminate lot-to-lot variability
  • Limit immunological reactions once cells are transplanted
  • Suitable for clinical applications
  • Ensure reliable performance
  • Eliminate the drawbacks of unwanted background differentiation and interruption in the cell metabolism 


For more details please visit: www.bioind.com/mesenchymal-stem-cells

Or contact us: info[at]bioind.com

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