These specialized supplements which are used instead of the standard kit supplement allow cryopreservation of freshly isolated or harvested cells in Nucleofector™ Solution.
“By decoupling cell harvesting and transfection, Nucleofection™ Experiments can be done at the scientist’s convenience. Cells can be collected, stored and are ready-to-use at a later time, and deliver transfection results comparable to fresh cells. Larger cell batches can be cryopreserved so that researchers can avoid batch-to-batch variances in transfection results. Thus, Nucleofector™ PLUS Supplements can make whole experimental programs more consistent.” explains Dr. Herbert Mueller-Hartmann, Head of R&D Discovery Technologies.
Nucleofection™ is a transfection technology that was first introduced into the market in 2001. It is based on the unique combination of electrical parameters and cell-type specific solutions. It is known as the first efficient non-viral transfection method for primary neural cells and has been stated in over 300 peer-reviewed publications as the method of choice for transfection of primary neurons.
Product, performance and packaging: The New Eppendorf cell culture consumables deliver quality in all and offer a new dimension of safe, reproducible and reliable cell culture work.
Scientists and technical personnel in the field of cell culture have a strong need for easy, safe and reliable products with improved handling that help prevent contamination. The latest products from Eppendorf deliver exceptional levels of product purity and security, as well as improved, ergonomic and safe handling of cell cultures and advanced protection against contamination. The new, easy resealable packaging concept complements the products’ unique features and performance.
Eppendorf Cell Culture Consumables are made from ultraclear virgin polystyrene, which complies with USP class VI for highest purity. Cell Culture Consumables have a ISO class /GMP class C clean room production standard and a sterility assurance level (SAL) of 10-6 as well as batch specific 3rd party quality testing ensuring exceptional product and sample safety.
Innovative technologies in Eppendorf Cell Culture Consumables deliver ultimate ease of use. New high efficiency air filter technology in Eppendorf flasks provides improved contamination protection, while ConvexAccess™ neck geometry allows more ergonomic handling and facilitated entry into flasks. Meanwhile, a corrugated handling ring and new SplashProtect™ ring in dishes deliver safe, confident handling and better protection against contamination. Finally, Eppendorf’s new chimney well design in plates minimizes the “edge effect” and leads to more reproducible assay results.
Many cell culture applications require the constant use of a sterile safety cabinet, which often has a limited amount of space available. Eppendorf’s new packaging concept addresses this limitation through easy opening, truly resealable and shrinkable design, allowing safe storage and requiring less space, while an innovative tray allows easy removal of the products from the box and safe workflows.
For convenient and secure sterile connection of flexible tubing in large volume operations.
Kleenpak II sterile connectors allow for the dry connection of two separate fluid pathways, while maintaining the sterile integrity of both, even in an uncontrolled environment. The connector consists of a male and a female connector, each covered by an aluminum peel-away strip that protects the port and maintains the sterility of the sterile fluid pathway prior to connection.
The Kleenpak II sterile connector has been designed to allow for the connection of large processing equipment in order to obtain fast, safe and reliable sterile transfer of large volumes.With this sterile connector, large single-use systems ( > 100 L), such as the AllegroTM systems, will be operated at much faster speeds. There will be minimal fluid restriction in thetubing lines.
Different connector options are available to allow for the connection of 19 mm (3⁄4 in.) and 25.4 mm (1 in.) ID nominal tubing including a 25.4 - 38.1 mm (1 - 11⁄2 in.) sanitary flange compatible fitting for maximum operational flexibility. Kleenpak II sterile connectors can be either gamma irradiated to a maximum of 50 kGy or autoclaved up to 130°C to suit different processing requirements.
The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards.
The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the µDAWN is similar to the well-established miniDAWN™ TREOS™, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.
Distinctly from the miniDAWN, the µDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the µDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.
The µDAWN may also be used in microbatch (off-line) mode or in conjunction with a Calypso IICG-MALS system for characterization of macromolecular interactions: affinity and absolute stoichiometry, label-free and immobilization-free, as well as second virial coefficients, cross virial coefficients, average molar masses and sizes.
GE Healthcare launches Cytell™ Cell Imaging System for compact, app-driven, automated imaging - Streamlines data acquisition, analysis and visualisation
Cytell™ Cell Imaging System is an intuitive benchtop instrument equipped with on-board data analysis and visualisation tools. It streamlines and simplifies routine assays such as cell cycle and cell viability and efficiently delivers high quality, quantitative results.
Key to the simplification are the on-board Bioapps; pre-configured, easy to use software modules that automate all the steps from imaging and data acquisition through data analysis, visualisation and report generation. The Cytell cell imaging system is compact and small enough to sit on a benchtop.
Cytell can image a wide variety of sample formats including slides, petri dishes, T-flasks and microplates. Compatible GE Healthcare Life Sciences reagents and kits are available, although Cytell is an open system and therefore provides the flexibility to create customized protocols based on other suppliers’ reagents.
BioApps currently available include:
Digital Imaging BioApp: Acquire fluorescence and transmitted light images of samples in plates, flasks, Petri dishes, or slides.
Automated Imaging BioApp: Automatically acquire high-quality fluorescence and transmitted light images from an entire multiwell plate in minutes.
Quick Count BioApp: Obtain accurate cell counts and viability and concentration estimations based on fluorescence intensity using a disposable hemocytometer.
Cell Cycle BioApp: Analyze cell cycle phase distribution in multi-well format using a DNA-binding fluorophore.
Cell Viability BioApp: Run two- or three-color assays in multiwell formats with subpopulation gating to determine the percentages of live, dead, and non-viable cells.
With improvements to the product and above all with enhanced documentation, the new minilab presents a huge number of new research opportunities for any user.
A new edition of the "lab in a suitcase"
For many years the Zinsser minilab has been an important component as a teaching aid in chemistry lessons in schools and universities. Now, with improvements to the product and above all with enhanced documentation, the new minilab presents a huge number of new research opportunities for any user.
Even though in the modern fine chemical industry and in pharmaceutical research and development where the smallest possible sample and reagent volumes are used (from a few mls and µls), for practical teaching, the experimentation often takes place in the front of the whole lesson with the use of larger volumes - up to 100mls - in schools and universities.
Containing an innovative set of glass equipment, the Mini Lab „lab in a suitcase“ is now used in many schools in different countries. There is now a new manual available for Mini Lab which includes more than 40 experiments - from basic operations such as distillation, filtration etc to complex syntheses such as colorants as designed by Prof. Michael Schallies. The instructions for the equipment configuration are easy to understand and are explained by simple drawings. General tips for the implementation of single experiments, material notes as well as danger symbols for the reagents are also included in the test description. The expected experimental results are also described in detail and partly documented with pictures or graphics. In the appendix of the manual there are detailed explanations of the potential dangers and also safety tips.
Current Owners of the Mini Lab can have a new updated manual for a small nominal charge.
Zinsser Analytic GmbH develops and manufactures in Germany and supplies customized automation technology and laboratory accessories for the global market. The company has specialized in the supply of high end technological products and systems for automated analysis in the fine chemical industry and also biological research and development. The company has grown over the years reaching a turnover in the range of double-digit millions and has about 60 employees to date.
Sartorius Stedim Biotech, a leading global supplier of the biopharmaceutical industry, is introducing a brand-new, scalable range of single-use bags.
Its completely new developed product family Flexsafe enables theimplementation of single-use bioprocessing throughout all steps of drug manufacture, fromprocess development to production, in upstream and downstream – all using just the oneinnovative polyethylene film. The innovative concept of the Flexsafe family addresses key industryrequirements for future-proof single-use manufacturing of commercial vaccines and drugs.
Flexsafe is based on a multilayer, proprietary polyethylene (PE) film, called S80, and has beendeveloped in close collaboration with resin and film suppliers. A standardized cell growthassay has been used to optimize film formulation, determine the operating ranges for extrusion,welding and gamma-irradiation processes and to establish specifications and process controls.
Flexsafe ensures excellent and reproducible cell growth behavior of the most sensitive celllines. The optimization of the resin formulation, the complete control of raw materials, theextrusion process and the bag assembly guarantee lot-to-lot consistent cell growthperformance. With its robust 400 μm thick PE film, Flexsafe is the strongest and most flexiblebag currently on the market. The bags enable safe and easy-to-use operation, even in themost demanding applications such as liquid shipping and large-scale stirred bioreactors.
Furthermore, batch-to-batch consistent extractables and leachables profiles support drugmanufacturers throughout the entire lifecycle of modern biological treatments from clinicaldevelopment to commercial supply many years after launch. Users can gain assurance thattheir initial extractable and leachable qualification work and data remain valid every timethey operate their single-use Flexsafe bioprocess.
Assurance of supply, one of the most important aspects for manufacturers, is guaranteed as aresult of long-term contracts with suppliers and business continuity plans with defined safetystocks and global manufacturing capabilities including resin manufacturing.
„Flexsafe represents a completely new generation of single-use bags. They offer our customersconsistent cell growth as well as extraordinary robustness and flexibility. In addition asustainable supply chain and consistent film throughout the entire manufacturing process isguaranteed. Every single one of these benefits sets a new benchmark. Being unique in all ofthem makes us very proud”, stated Stefan Schlack, Senior Vice President Marketing andProduct Management at Sartorius Stedim Biotech.
Sartorius Stedim Biotech is launching Flexsafe RM bags (1L-200L) and small bags for validationpurposes first. Bags for single use bioreactors BIOSTAT STR (50-2000L) and additional applications such as storage, mixing, shipping freeze and thaw will be rolled out step by step.
AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.
In contrast to conventional in-vitro diagnostic methods, AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.
The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.
The ProdX software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient.
Seamless Integration of Product Inspection Devices
Whatever your role in product inspection management, you will have plenty of challenges to face in marketplaces that increasingly demand more for less.
ProdX Inspect data management software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient. ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
ProdX Savings Calculator
Calculate your time savings potential with ProdX
This Calculator runs through all costs involved in the setup and data collection processes. It calculates the amount you could save per year by comparing the time requirement of manual processes and the automated data collection process this software offers.
ProdX Quality Management & AssuranceThe innovative ProdX software automatically collects the critical inspection process data you need in one convenient location significantly reducing the need to interact directly with the Critical Control Points on your factory floor. This saves time and effort enabling quality operatives to focus on other issues.
ProdX Production Process Managment
ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
PromoKine offers a wide range of products for cell analysis including numerous kits for fast, convenient and sensitive measurement of cell viability/proliferation, cytotoxicity, cell death and apoptosis, cell stress and metabolism as well as reporter gene expression.
PromoKine’s Fluorometric Cell Viability Kits are based on resazurin or calcein-AM conversion while our Colorimetric Cell Viability Kits utilize reduction of different tetrazolium salts (e.g. MTT, XTT, WST-1 & WST-8), and the Bioluminescent Cell Viability Kits measure ATP levels as a marker for cell viability in a luciferase assay.
Our Cytotoxicity Kits quantify the amount of lactate dehydrogenase (LDH) or adenylate kinase (AK) released from damaged cells either colorimetrically or by using a luminometer.
PromoKine’s Apoptosis Assays include various kits for detection and quantitation of e.g., caspase activity, annexin V binding, DNA fragmentation or mitochondrial changes. Moreover, PromoKine provides Reporter Assay Kits (beta-galactosidase, luciferase, GFP) as well as a selection of small-sized reporter plasmids and fusion plasmids (target gene fused with GFP or luciferase gene).
In addition, you will find in our catalog and on our website a wide range of other interesting products for cell biology research such as numerous high-quality and economically priced recombinant proteins (e.g. caspases, kinases, cytokines, chemokines & growth factors), a variety of primary and secondary antibodies and ELISA kits as well as premium, user-friendly reagents and kits for optimal cell transfection, fluorescent staining of cell structures, labeling of biomolecules (proteins, antibodies and nucleic acids) and the detection, elimination and prevention of mycoplasma and bacterial contamination in cell culture.
ReadyTector is an easy, quick and clear all-in-one detection solution for Western blotting.
All-in-one solution for Western blots
The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously.
The only additional step is washing with the specific ReadyTector Wash Buffer.
ReadyTector for Western blotting is easy, quick and clear:
easy – very simple procedure
quick – very fast procedure with very quick results
clear – no background
The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.