Product of the Week

Ni Sepharose excel media

GE Healthcare

Ni Sepharose excel media

His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.

Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells. 

Key features:

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several different formats for screening and preparative purification of histidine-tagged proteins 


IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.

Contact Information:

GE Healthcare Europe GmbH

Life Sciences

Andrea Löhndorf, PhD, MBA

Regional Marketing Manager

Mobil: +49 (0)174 92 36 400


PromoCell GmbH

PromoKine is now adding more than 200 new ELISA kits for target molecules which play integral roles in cell signaling research.

PromoCell GmbH

PromoKine currently offers ~400 ELISA kits for detecting and quantifying the "free" forms of human, mouse, rat, monkey and pig cytokines, growth factors and other cell biology target molecules in cell culture supernatants, plasma, serum and other body fluids.  Some can even be used with tissue lysates! Our ready-to-use ELISA kits are very convenient, including a user-friendly and easy-to-follow protocol along with all the necessary components to perform a highly sensitive, reliable and reproducible ELISA.

In addition, we also provide cost-effective ELISA Development Kits containing all required reagents to assay your target protein/peptide in ten 96-well microtiter plates (approximately 1,000 assays per kit).

PromoKine is now adding more than 200 new ELISA kits for target molecules which play integral roles in cell signaling research; such as diverse interleukins, FGFs, CD markers, BMPs, MIPs, MMPs and MCPs as well as Activin A, beta-NGF, Cardiotrophin, Lymphotactin, Oncostatin M, Insulin and several other hormones. We also have launched a new line of Cell-based Phosphorylation ELISAs.

Along with this, we are extending our comprehensive product line of recombinant cytokines, growth factors, soluble receptors, hormones and other proteins/peptides involved in cell signaling as well as our wide range of antibodies against these molecules.

Test our new ELISAs and get one of our highly coveted t-shirts for free. See our current promotion at

More Information: 

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg



The Biometra GmbH, a company of the Analytik Jena AG, now offers illuminators with high-performance blue light LEDs for gel documentation purposes.


Blue light transilluminators are a quite interesting alternative to UV tables as there is no risk of sample damage during illumination. This is important when samples shall be processed furthermore after gel documentation. Users also benefit from it as there is no risk of UV exposure. Blue light excitation is applicable for fluorescent dyes for nucleic acid or protein stains with excitation wavelength around 470 nm. Examples for compatible stains are: SYBR® Green, GelGreenTM, SYBR® Safe, SYBR® Gold or SYPRO® Ruby. The amber lid of the instrument allows the direct view to the fluorescent samples. There is no need for separate glasses. 
The new blue light illuminators are available in two versions: "BLstar 9", the handy solution for mini gels and "BLstar 16" for gels up to size of 16 cm x 20 cm.

Contact information:

Hanna Engelhardt

Media Relations Life Science

Tel: +49 36 41 77-73 55

Fax: +49 36 41 77-92 79



The Operetta CLS system enables scientists to gain deep biological insight into disease from common assays and complex applications using advanced cellular models, and to identify even subtle phenotypic changes through sensitive imaging and intuitive data analysis.


The Operetta CLS system enables scientists to gain deep biological insight into disease from common assays and complex applications using advanced cellular models, and to identify even subtle phenotypic changes through sensitive imaging and intuitive data analysis.

Speed, sensitivity and high resolution are achieved through the unique combination of automated water immersion objectives that deliver better quality image data, stable LED illumination, true confocal optics and a sensitive, high resolution sCMOS camera.  

Controlled by PerkinElmer’s Harmony® high-content imaging and analysis software that provides a simple-to-use, streamlined workflow, researchers can utilize the system to conduct their own analysis and customize as needed to an application.

Find out more at

Thermo Fisher Scientific

Thermo Fisher Scientific releases new small benchtop centrifuge, which provides scientists with outstanding capacity in a compact design.

Thermo Fisher Scientific

Thermo Fisher Scientific Inc. has launched a new small benchtop centrifuge to meet clinical and research needs alike.

“Centrifuges are in constant use and demand so preventing downtime and bottlenecks is crucial,” said Maurizio Merli, product director, Thermo Fisher Scientific. “With features designed for simplicity, safety and flexibility, the new small benchtop centrifuges can help scientists save time and maximize resources.”

The new centrifuges provide:

  • Application flexibility, convenient cleaning and ease-of-use with Thermo Scientific Auto-Lock rotor exchange
  • Versatility to spin blood tubes, conical tubes, microtubes and microplate compatibility with sealing options
  • One-handed sample protection with Thermo Scientific ClickSeal biocontainment lids, certified by the Public Health Laboratory Service in Porton Down
  • Intuitive glove and detergent-friendly interface with a bright display, one-touch operation with pre-set protocol options and optional password protection

More information:

Phone +44 870 609 9203

PromoCell GmbH

Efficient testing of the pro- or anti-angiogenic capacity of substances and drugs demands for standardized and easy-to-use test systems that deliver reproducible data quickly.

PromoCell GmbH

PromoCell provides four different ready-to-use angiogenesis cell model systems. Each of them allows the simulation of angiogenesis processes, by detailed in vitro recapitulation of the biological events involved in de novo vessel formation and remodelling: proteolytic activity, migration, proliferation and tube formation.

The PromoCell 3D Angiogenesis Kit examines all process together. It contains a collagen matrix with embedded endothelial cell spheroids and arrives ready-to-use in your lab. The only thing you need to do in order to start the assay is to add your test substance. Following proper stimulation de novo vessel formation occurs within two days, visible by sprouting of tubes into the surrounding matrix. The number and length of the sprouts corresponds to the pro- or anti-angiogenic potency of the test substance. Evaluation of the results is straightforward and easy, and staining procedures are not necessary.

To investigate the separate events of angiogenesis in a simulated in vitro environment, the PromoCell Transmigration Kit, the PromoCell Invasion Kit and the PromoCell Planar Migration Assay are useful platforms.

The PromoCell Transmigration Kit takes advantage of the ability of endothelial cells to migrate along cytokine gradients in a concentration-dependent manner, allowing for the measurement of the chemotactic potential of a test substance.

The PromoCell Invasion Kit is suitable for in vitro simulation of the proteolytic activity of endothelial cells as well as investigating directed migration. Hence, the impact of test substances on one or both of the aforementioned parameters can be easily monitored.

The PromoCell Planar Migration Assay is a 2D test platform for rapid evaluation of the influence of test substances on the migratory response of endothelial cells.

Contact Information:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg


+49 (0)6221-649340


Dunn Labortechnik

Art Robbins Instruments has converted - together with the American company SciGene - the Scorpion workstation to automate MicroFISH slide preparation.

Dunn Labortechnik

Art Robbins Instruments has converted - together with the American company SciGene - the Scorpion workstation to automate MicroFISH slide preparation. MicroFISH is a miniaturized, slide-based FISH assay that reduces probe cost per test up to tenfold and the labour used to prepare and process assay slides by over 60 %. The MicroFISH assay uses a multi-well slide for performing up to eight independent microvolume FISH assays using only 1 µl of cell sample and probe. The Scorpion, with it simple step-by-step interface, streamlines the workflow and increases quality control by automating slide dropping and probe application and providing inventory tracking and batch reports with probe lot number and expiration dates.

The Scorpion MicroFISH with slides and suitable process equipment will be shown for the first time in Europe on the 29th Tumorgenetics Workshop from 19th to 21st May in the Leonardo Royal Hotel in Munich at the booth of our partner AlphaMetrix Biotech.

For further information please contact:

Dunn Labortechnik GmbH

Thelenberg 6

53567 Asbach

Tel. +49 (2683) 4 30 94

Fax +49 (2683) 4 27 76

E-Mail: info[at]


The experience acquired with one of its clients allows 3P the capability to offer this alternative technology in its services pipeline.


The Upstream and the Technical Services teams have headed a cell free process; this is a state of the art technology that allows obtaining a specific enzyme in a non-stop fermentation process. This is possible thanks to an enzymatic biochemical reaction without any cellular mechanism interaction. 

This is an important landmark for the company in several aspects: 

  1. The successful coverage of a continuous production processes.
  2. Control of temperature during all stages to ensure stability throughout the process with adaptation of equipments and facilities to guarantee this condition
  3. The scaling-up of this technology with the singularity of maintaining constantly the temperature to guarantee the reproducibility of the process.
  4. The development of this alternative technology opens the door to those potential clients who need this type of resources to produce proteins and small molecules and sets the company in a leading position in the global biotechnology industry.

About 3PBiopharmaceuticals. 

3P is a CDMO organization that develops and manufactures biological and cellular therapy products for preclinical, clinical trials and commercial phases for other companies under GMP conditions.

Contact Information:

Edurne Gil  

3P Biopharmaceuticals Communications Manager

Tel.: + 34 984 346480

eMail: egil[at]



ReadyTector is an easy, quick and clear all-in-one detection solution for Western blotting.

Candor Bioscience GmbH

All-in-one solution for Western blots

The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously. 

The only additional step is washing with the specific ReadyTector Wash Buffer.

ReadyTector for Western blotting is easy, quick and clear:

  • easy – very simple procedure
  • quick – very fast procedure with very quick results
  • clear – no background

The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.


Candor Bioscience GmbH

Simoniusstrasse 39

88239 Wangen, Germany

Tel.: +49(0)-7522-7952-70

Fax: +49(0)-7522-7952-729


Pall Life Sciences

96-well filter plates pre-filled with a family of chromatography sorbents for sorbent screening, or to optimize conditions in high throughput mode for protein purification.

Pall Life Sciences

AcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with Pall chromatography sorbents, to enable early chromatography sorbent screening for protein purification. Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption. Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using Pall 1 mL or 5 mL PRC prepacked columns filled with the same sorbents. 

AcroPrep ScreenExpert plate packages offer:

Flexibility: One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents or two optimization plates: one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent - You select your preference.
Easy Scale Up: Perform Design of Experiments (DoE) in 96-well plates and transfer to column chromatography. 1 mL or 5 mL PRC pre-packed columns filled with relevant sorbents allow convenient verification and small preparative work.
Minimized Sample Consumption: 50 μL of sorbent per well, allowing small sample usage. 1 mL well volume and 100 to 500 μL working volume.
Convenience: A disposable format in a convenient individual package. Compatible with manual multi-well plate vacuum manifold or robotic workstation-assisted operations.

Click here to learn more.

Biological Industries

Isolate, grow, maintain and differentiate human mesenchymal stem cells from various sources with a complete, optimized culture system.

Biological Industries

Biological Industries offers a full product portfolio, including stem cell culture media, freezing media, attachment solution, cell dissociation solutions and initial differentiation kits. 

Isolation and Culture

MSC NutriStem® XF line and its supporting reagents are optimized for human mesenchymal stem cells from a variety of tissue sources and promise optimal growth and maintenance of hMSC, high proliferation rate, superior maintenance of cell characteristics, fibroblast-like morphology maintenance and long-term growth


MSC GoTM Osteogenic, Adipogenic and Chondrogenic  XF series offer a complete system for the evaluation and  reliable induction of hMSC from various sources into adipocytes, chondrocytes and osteoblasts/osteocytes. The kits are designed to be user friendly with all the growth factors and supplements necessary for the direct differentiation of hMSC

All products are completely free of serum and non-human derived components, hence:

  • Eliminate contagious health risks from viral agents, mycoplasma, and prions
  • Eliminate lot-to-lot variability
  • Limit immunological reactions once cells are transplanted
  • Suitable for clinical applications
  • Ensure reliable performance
  • Eliminate the drawbacks of unwanted background differentiation and interruption in the cell metabolism 

For more details please visit:

Or contact us: info[at]

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