Product of the Week

Ni Sepharose excel media

GE Healthcare

Ni Sepharose excel media

His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.

Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells. 

Key features:

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several different formats for screening and preparative purification of histidine-tagged proteins 


IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.

Contact Information:

GE Healthcare Europe GmbH

Life Sciences

Andrea Löhndorf, PhD, MBA

Regional Marketing Manager

Mobil: +49 (0)174 92 36 400

Thermo Fisher Scientific

Thermo Fisher Scientific releases new small benchtop centrifuge, which provides scientists with outstanding capacity in a compact design.

Thermo Fisher Scientific

Thermo Fisher Scientific Inc. has launched a new small benchtop centrifuge to meet clinical and research needs alike.

“Centrifuges are in constant use and demand so preventing downtime and bottlenecks is crucial,” said Maurizio Merli, product director, Thermo Fisher Scientific. “With features designed for simplicity, safety and flexibility, the new small benchtop centrifuges can help scientists save time and maximize resources.”

The new centrifuges provide:

  • Application flexibility, convenient cleaning and ease-of-use with Thermo Scientific Auto-Lock rotor exchange
  • Versatility to spin blood tubes, conical tubes, microtubes and microplate compatibility with sealing options
  • One-handed sample protection with Thermo Scientific ClickSeal biocontainment lids, certified by the Public Health Laboratory Service in Porton Down
  • Intuitive glove and detergent-friendly interface with a bright display, one-touch operation with pre-set protocol options and optional password protection

More information:

Phone +44 870 609 9203

PromoCell GmbH

Efficient testing of the pro- or anti-angiogenic capacity of substances and drugs demands for standardized and easy-to-use test systems that deliver reproducible data quickly.

PromoCell GmbH

PromoCell provides four different ready-to-use angiogenesis cell model systems. Each of them allows the simulation of angiogenesis processes, by detailed in vitro recapitulation of the biological events involved in de novo vessel formation and remodelling: proteolytic activity, migration, proliferation and tube formation.

The PromoCell 3D Angiogenesis Kit examines all process together. It contains a collagen matrix with embedded endothelial cell spheroids and arrives ready-to-use in your lab. The only thing you need to do in order to start the assay is to add your test substance. Following proper stimulation de novo vessel formation occurs within two days, visible by sprouting of tubes into the surrounding matrix. The number and length of the sprouts corresponds to the pro- or anti-angiogenic potency of the test substance. Evaluation of the results is straightforward and easy, and staining procedures are not necessary.

To investigate the separate events of angiogenesis in a simulated in vitro environment, the PromoCell Transmigration Kit, the PromoCell Invasion Kit and the PromoCell Planar Migration Assay are useful platforms.

The PromoCell Transmigration Kit takes advantage of the ability of endothelial cells to migrate along cytokine gradients in a concentration-dependent manner, allowing for the measurement of the chemotactic potential of a test substance.

The PromoCell Invasion Kit is suitable for in vitro simulation of the proteolytic activity of endothelial cells as well as investigating directed migration. Hence, the impact of test substances on one or both of the aforementioned parameters can be easily monitored.

The PromoCell Planar Migration Assay is a 2D test platform for rapid evaluation of the influence of test substances on the migratory response of endothelial cells.

Contact Information:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg


+49 (0)6221-649340


The experience acquired with one of its clients allows 3P the capability to offer this alternative technology in its services pipeline.


The Upstream and the Technical Services teams have headed a cell free process; this is a state of the art technology that allows obtaining a specific enzyme in a non-stop fermentation process. This is possible thanks to an enzymatic biochemical reaction without any cellular mechanism interaction. 

This is an important landmark for the company in several aspects: 

  1. The successful coverage of a continuous production processes.
  2. Control of temperature during all stages to ensure stability throughout the process with adaptation of equipments and facilities to guarantee this condition
  3. The scaling-up of this technology with the singularity of maintaining constantly the temperature to guarantee the reproducibility of the process.
  4. The development of this alternative technology opens the door to those potential clients who need this type of resources to produce proteins and small molecules and sets the company in a leading position in the global biotechnology industry.

About 3PBiopharmaceuticals. 

3P is a CDMO organization that develops and manufactures biological and cellular therapy products for preclinical, clinical trials and commercial phases for other companies under GMP conditions.

Contact Information:

Edurne Gil  

3P Biopharmaceuticals Communications Manager

Tel.: + 34 984 346480




ReadyTector is an easy, quick and clear all-in-one detection solution for Western blotting.

Candor Bioscience GmbH

All-in-one solution for Western blots

The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously. 

The only additional step is washing with the specific ReadyTector Wash Buffer.

ReadyTector for Western blotting is easy, quick and clear:

  • easy – very simple procedure
  • quick – very fast procedure with very quick results
  • clear – no background

The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.


Candor Bioscience GmbH

Simoniusstrasse 39

88239 Wangen, Germany

Tel.: +49(0)-7522-7952-70

Fax: +49(0)-7522-7952-729

Pall Life Sciences

96-well filter plates pre-filled with a family of chromatography sorbents for sorbent screening, or to optimize conditions in high throughput mode for protein purification.

Pall Life Sciences

AcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with Pall chromatography sorbents, to enable early chromatography sorbent screening for protein purification. Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption. Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using Pall 1 mL or 5 mL PRC prepacked columns filled with the same sorbents. 

AcroPrep ScreenExpert plate packages offer:

Flexibility: One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents or two optimization plates: one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent - You select your preference.
Easy Scale Up: Perform Design of Experiments (DoE) in 96-well plates and transfer to column chromatography. 1 mL or 5 mL PRC pre-packed columns filled with relevant sorbents allow convenient verification and small preparative work.
Minimized Sample Consumption: 50 μL of sorbent per well, allowing small sample usage. 1 mL well volume and 100 to 500 μL working volume.
Convenience: A disposable format in a convenient individual package. Compatible with manual multi-well plate vacuum manifold or robotic workstation-assisted operations.

Click here to learn more.

Biological Industries

Isolate, grow, maintain and differentiate human mesenchymal stem cells from various sources with a complete, optimized culture system.

Biological Industries

Biological Industries offers a full product portfolio, including stem cell culture media, freezing media, attachment solution, cell dissociation solutions and initial differentiation kits. 

Isolation and Culture

MSC NutriStem® XF line and its supporting reagents are optimized for human mesenchymal stem cells from a variety of tissue sources and promise optimal growth and maintenance of hMSC, high proliferation rate, superior maintenance of cell characteristics, fibroblast-like morphology maintenance and long-term growth


MSC GoTM Osteogenic, Adipogenic and Chondrogenic  XF series offer a complete system for the evaluation and  reliable induction of hMSC from various sources into adipocytes, chondrocytes and osteoblasts/osteocytes. The kits are designed to be user friendly with all the growth factors and supplements necessary for the direct differentiation of hMSC

All products are completely free of serum and non-human derived components, hence:

  • Eliminate contagious health risks from viral agents, mycoplasma, and prions
  • Eliminate lot-to-lot variability
  • Limit immunological reactions once cells are transplanted
  • Suitable for clinical applications
  • Ensure reliable performance
  • Eliminate the drawbacks of unwanted background differentiation and interruption in the cell metabolism 

For more details please visit:

Or contact us:

Life Technologies

This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time.

Life Technologies

From creating your gene to expressing your protein, Life Technologies offers a wide range of tools and expression systems that are easy to use, help enable rapid results, and offer high protein yields for every downstream application. This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time compared to using traditional methods. We even offer a complete service for the entire gene-to-protein workflow.

Request a copy of the Gene to Protein Brochure today by filling out the form here!

Or download the PDF version here!

Contactinformation for GeneArt® products and services:


Europe: Tel: +49 (0)941 942 76100 Fax: +49 (0)941 942 76780

America and Asia: Tel: 800 955 6288 option 4/4/1

Wyatt Technology

The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards.

Wyatt Technology Corporation

The µDAWN™  Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the µDAWN is similar to the well-established miniDAWN™ TREOS™, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.

Distinctly from the miniDAWN, the µDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the µDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.

The µDAWN and its companion Optilab® UT-rEX™ Refractive Index Detector interface to most standard UHPLC systems. As these instruments are based on the advanced technology of the miniDAWN TREOS and Optilab T-rEX, respectively, users may be confident in the underlying capabilities cited in thousands of peer-reviewed publications

The µDAWN may also be used in microbatch (off-line) mode or in conjunction with a Calypso II CG-MALS system for characterization of macromolecular interactions: affinity and absolute stoichiometry, label-free and immobilization-free, as well as second virial coefficients, cross virial coefficients, average molar masses and sizes.


Wyatt Technology Europe GmbH

Dr. Thomas Jocks | Scientific Marketing

Hochstrasse 18 | 56307 Bernbach | Germany

Tel.: +49(0)2689-925-215 | Fax: +49(0)2689-925-299

Web: www.wyatt.EU | Email: thomas.jocks@wyatt.EU


Today, embryonic stem cells (ESC) and induced pluripotent stem cells (iPS) represent the most significant types of pluripotent stem cells (PSC).

PromoCell GmbH

In the beginning, ESC were cultured on feeder cell layers, primarily murine embryonic fibroblasts (MEF), in non-defined culture media containing fetal calf serum (FCS). Later on, FCS would be replaced by somewhat more defined serum replacements and the introduction of extracellular matrix (ECM) coatings supporting self-renewal of ESC allowed for feeder-free culture using feeder-cell conditioned medium. However, the culture process remained time-consuming, laborious and poorly defined.

In recent years PSC-culture has experienced significant technical progress. Chemically defined media compositions became a standard, followed by the first xeno-free defined formulations. However, all these culture systems still depended on non-defined, xenogenic and poorly standardized ECM preparations – a contradiction in terms when using defined / xeno-free media. Importantly, research recently overcame this hurdle, identifying natural as well as synthetic ECM molecules and peptides that support undifferentiated PSC-expansion. Scientists are now capable of completely defined / humanized derivation and culture of hPSC.

Despite these recent technical advancements, the established hPSC culture systems still share unfavourable properties. Most of them use supra-physiologically high amounts of growth factors and / or contain substances purified from human or animal origin. In addition animal-derived and / or non-defined ECM is used. The PromoCell hPSC Growth Medium DXF consequently eliminates these disadvantages:

  • Chemically defined / xeno-free complete culture system
  • No human / animal derived components
  • Physiologically low in growth factors

This optimal culture environment allows for a well-controlled culture process, consistent and reproducible performance, robust support of pluripotency and improved cloning efficiency.

In combination with the extracellular matrix hPSC-ECM DXF and the hPSC Dissociation Buffer DXF, PromoCell provides a defined and xeno-free complete culture system for human pluripotent stem cells.


PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg


+49 6221 649 34 0


With 100g samples, the Peptone Starter Paks allow for you to identify the correct supplements for your specific cell line faster.

BD Biosciences

BD is helping you rethink supplementation. For more than 100 years, leading pharmaceutical companies have trusted BD peptones and supplements to help improve cell culture performance with higher cell titers and yield.

The new BD Peptone Starter Paks provide samples of our most widely used peptones tailored for specific applications, including monoclonal antibodies, recombinant proteins, and vaccines, for use in both mammalian cell culture and microbial fermentation.

With 100g samples conveniently packed in one box, you can identify the correct supplements for your specific cell line faster.

Request a starter pak today and start maximizing your cell’s performance. Go to

Peptone Starter Paks are for evaluation use only.



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