Product of the Week

Ni Sepharose excel media
Vergrößern

GE Healthcare

Ni Sepharose excel media

His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.

Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells. 

Key features:

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several different formats for screening and preparative purification of histidine-tagged proteins 

Background:

IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.


Contact Information:

GE Healthcare Europe GmbH

Life Sciences

Andrea Löhndorf, PhD, MBA

Regional Marketing Manager

Mobil: +49 (0)174 92 36 400

andrea.loehndorf@ge.com

www.gelifesciences.com

SARTORIUS

Sartorius Stedim Biotech, a leading global supplier of the biopharmaceutical industry, is introducing a brand-new, scalable range of single-use bags.

Sartorius Stedim Biotech

Its completely new developed product family Flexsafe enables theimplementation of single-use bioprocessing throughout all steps of drug manufacture, fromprocess development to production, in upstream and downstream – all using just the oneinnovative polyethylene film. The innovative concept of the Flexsafe family addresses key industryrequirements for future-proof single-use manufacturing of commercial vaccines and drugs.

Flexsafe is based on a multilayer, proprietary polyethylene (PE) film, called S80, and has beendeveloped in close collaboration with resin and film suppliers. A standardized cell growthassay has been used to optimize film formulation, determine the operating ranges for extrusion,welding and gamma-irradiation processes and to establish specifications and process controls.

Flexsafe ensures excellent and reproducible cell growth behavior of the most sensitive celllines. The optimization of the resin formulation, the complete control of raw materials, theextrusion process and the bag assembly guarantee lot-to-lot consistent cell growthperformance. With its robust 400 μm thick PE film, Flexsafe is the strongest and most flexiblebag currently on the market. The bags enable safe and easy-to-use operation, even in themost demanding applications such as liquid shipping and large-scale stirred bioreactors.

Furthermore, batch-to-batch consistent extractables and leachables profiles support drugmanufacturers throughout the entire lifecycle of modern biological treatments from clinicaldevelopment to commercial supply many years after launch. Users can gain assurance thattheir initial extractable and leachable qualification work and data remain valid every timethey operate their single-use Flexsafe bioprocess.

Assurance of supply, one of the most important aspects for manufacturers, is guaranteed as aresult of long-term contracts with suppliers and business continuity plans with defined safetystocks and global manufacturing capabilities including resin manufacturing.

„Flexsafe represents a completely new generation of single-use bags. They offer our customersconsistent cell growth as well as extraordinary robustness and flexibility. In addition asustainable supply chain and consistent film throughout the entire manufacturing process isguaranteed. Every single one of these benefits sets a new benchmark. Being unique in all ofthem makes us very proud”, stated Stefan Schlack, Senior Vice President Marketing andProduct Management at Sartorius Stedim Biotech.

Sartorius Stedim Biotech is launching Flexsafe RM bags (1L-200L) and small bags for validationpurposes first. Bags for single use bioreactors BIOSTAT STR (50-2000L) and additional applications such as storage, mixing, shipping freeze and thaw will be rolled out step by step.


Contact:

Dominic Grone

Group Corporate Communications

Sartorius AG

Goettingen

Phone:+49.(0)551.308.3324

email: dominic.grone@sartorius.com

web: www.sartorius.com

CETICS

AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.

CETICS Healthcare Technologies GmbH

In contrast to conventional in-vitro diagnostic methods,  AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.

The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.

Fields of application:

  • Toxicology
  • Cell Culture Quality Control
  • Pharmaceutical Development


Ask us for additional information:  

CETICS Healthcare Technologies GmbH

Schelztorstr. 54-56

73728 Esslingen am Neckar

Germany

Phone:+49 (0)711 300 655-0

Fax:+49 (0)711 300 655-29

e-mail: info@cetics-ht.com

Mettler Toledo

The ProdX software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient.

Mettler Toledo

Seamless Integration of Product Inspection Devices

Whatever your role in product inspection management, you will have plenty of challenges to face in marketplaces that increasingly demand more for less.

ProdX Inspect data management software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient. ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.

ProdX Savings Calculator

Calculate your time savings potential with ProdX

This Calculator runs through all costs involved in the setup and data collection processes. It calculates the amount you could save per year by comparing the time requirement of manual processes and the automated data collection process this software offers. 

ProdX Quality Management & AssuranceThe innovative ProdX software automatically collects the critical inspection process data you need in one convenient location significantly reducing the need to interact directly with the Critical Control Points on your factory floor. This saves time and effort enabling quality operatives to focus on other issues.

ProdX Production Process Managment

ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.


For further information please click here.

PromoKine

PromoKine offers a wide range of products for cell analysis including numerous kits for fast, convenient and sensitive measurement of cell viability/proliferation, cytotoxicity, cell death and apoptosis, cell stress and metabolism as well as reporter gene expression.

PromoCell GmbH

PromoKine’s Fluorometric Cell Viability Kits are based on resazurin or calcein-AM conversion while our Colorimetric Cell Viability Kits utilize reduction of different tetrazolium salts (e.g. MTT, XTT, WST-1 & WST-8), and the Bioluminescent Cell Viability Kits measure ATP levels as a marker for cell viability in a luciferase assay. 

Our Cytotoxicity Kits quantify the amount of lactate dehydrogenase (LDH) or adenylate kinase (AK) released from damaged cells either colorimetrically or by using a luminometer.

PromoKine’s Apoptosis Assays include various kits for detection and quantitation of e.g., caspase activity, annexin V binding, DNA fragmentation or mitochondrial changes.
Moreover, PromoKine provides Reporter Assay Kits (beta-galactosidase, luciferase, GFP) as well as a selection of small-sized reporter plasmids and fusion plasmids (target gene fused with GFP or luciferase gene).

In addition, you will find in our catalog and on our website a wide range of other interesting products for cell biology research such as numerous high-quality and economically priced recombinant proteins (e.g. caspases, kinases, cytokines, chemokines & growth factors), a variety of primary and secondary antibodies and ELISA kits as well as premium, user-friendly reagents and kits for optimal cell transfection, fluorescent staining of cell structures, labeling of biomolecules (proteins, antibodies and nucleic acids) and the detection, elimination and prevention of mycoplasma and bacterial contamination in cell culture.

For more information and pricing please visit our website: www.promokine.info


Contact:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

Germany

Tel.: +49 6221 - 649 34 0

Fax: +49 6221 - 649 34 40

www.promokine.info
info@promokine.de

CANDOR

ReadyTector is an all-in-one detection solution for Western blotting.

Candor Bioscience GmbH

All-in-one solution for Western blots

The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously. 

The only additional step is washing with the specific ReadyTector Wash Buffer.

ReadyTector for Western blotting is easy, quick and clear:

# easy – very simple procedure

# quick – very fast procedure with very quick results

# clear – no background

The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.


Contact:

Phone: +49-7522-7952-713

www.readytector.com

Avestin

The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes.

AVESTIN

1. Principle of Operation 

The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes. The sample is passed through the membrane by pushing the sample back and forth between two syringes. 

2. Temperature Control 

The entire LiposoFast-Basic can be immersed in a water bath for use with high transition temperature lipids or heat sensitive compounds. 

3. Accessories 

The optional LiposoFast-Stabilizer was designed to simplify the repetitive use of the LiposoFast-Basic as well as the extrusion of highly concentrated samples. It accommodates both 0.5mL and 1.0mL syringes. 

4. Free Trial 

This instrument is available for a free trial. Simply contact us and we can send it to you. If after the trial you are not satisfied with it or it does not suit your research, simply send it back.


Contact:

Avestin Europe GmbH

Weinheimer Straße 64b

68309 Mannheim 

Deutschland

Michael Fischer

Tel. +49 (0621) 724-5980

Fax +49 (0621) 724-5813

avestin@avestin.net

www.avestin.com 

PromoCell

Today, embryonic stem cells (ESC) and induced pluripotent stem cells (iPS) represent the most significant types of pluripotent stem cells (PSC).

PromoCell GmbH

In the beginning, ESC were cultured on feeder cell layers, primarily murine embryonic fibroblasts (MEF), in non-defined culture media containing fetal calf serum (FCS). Later on, FCS would be replaced by somewhat more defined serum replacements and the introduction of extracellular matrix (ECM) coatings supporting self-renewal of ESC allowed for feeder-free culture using feeder-cell conditioned medium. However, the culture process remained time-consuming, laborious and poorly defined.

In recent years PSC-culture has experienced significant technical progress. Chemically defined media compositions became a standard, followed by the first xeno-free defined formulations. However, all these culture systems still depended on non-defined, xenogenic and poorly standardized ECM preparations – a contradiction in terms when using defined / xeno-free media. Importantly, research recently overcame this hurdle, identifying natural as well as synthetic ECM molecules and peptides that support undifferentiated PSC-expansion. Scientists are now capable of completely defined / humanized derivation and culture of hPSC.

Despite these recent technical advancements, the established hPSC culture systems still share unfavourable properties. Most of them use supra-physiologically high amounts of growth factors and / or contain substances purified from human or animal origin. In addition animal-derived and / or non-defined ECM is used. The PromoCell hPSC Growth Medium DXF consequently eliminates these disadvantages:

  • Chemically defined / xeno-free complete culture system
  • No human / animal derived components
  • Physiologically low in growth factors

This optimal culture environment allows for a well-controlled culture process, consistent and reproducible performance, robust support of pluripotency and improved cloning efficiency.

In combination with the extracellular matrix hPSC-ECM DXF and the hPSC Dissociation Buffer DXF, PromoCell provides a defined and xeno-free complete culture system for human pluripotent stem cells.

Contact:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

Germany

+49 6221 649 34 0

www.promokine.info

info@promokine.info

Eppendorf

The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level.

Eppendorf

The BioBLU 0.3f and BioBLU 1f Single-use Vessels allow microbial process development at the highest level. These rigid wall stirred-tank single-use vessels have been specifically designed for high cell density fermentation with bacteria, fungi and yeast. BioBLU f vessels can be used with the Eppendorf DASbox® and DASGIP® Parallel Bioreactor Systems.

Compared to cell culture applications, fermentation processes require much higher kLa values for proper mass transfer as well as suitable heating and cooling options. Proven stirred-tank design, powerful overhead drives featuring Rushton-type impellers and innovative baffles for cooling make the BioBLU f vessels achieve these demands.

  • Reliable scalability through industrial design
  • Sealed magnetic overhead drives with encapsulated bearings and Rushton-type impellers for excellent mixing
  • High performance mass & heat transfer suitable for high cell density fermentation
  • Minimal set-up times and most easy handling
  • Liquid-free Peltier exhaust condenser
  • All wetted materials animal component free and USP Class VI certified
  • Working volumes: 65 – 250 mL (BioBLU 0.3f) and 250 mL – 1.25 L (BioBLU 1f)

Combine the advantages of single-use bioreactor technology with the reliable performance of conventional glass or stainless steel bioreactors for your fermentation – Discover the Eppendorf BioBLU f product line: www.eppendorf.com/BioBLUf


Contact:

Eppendorf AG

Barkhausenweg 1

22339 Hamburg

Germany

Tel: +49 40 53801-0

E-Mail: bioprocess-info@eppendorf.de

www.eppendorf.com

Gilson

PlateMaster is an amazingly easy to use, accurate solution for high throughput pipetting of 96 and 384 well plates.

Gilson

PlateMaster is an amazingly easy to use, accurate solution for high throughput pipetting of 96 and 384 well plates. Its compact and ergonomic design means it can be used anywhere and by anyone for fast, effortless and reproducible pipetting of 96 channels.

Simple Operation

The manual 96-channel pipetting PlateMaster can easily be operated by everyone after an introduction of just 5 minutes. Being a manually controlled instrument there is no complicated and time-consuming programming. The operation is as easy and intuitive as with a manual pipette.

Fill a plate in a single dispense!

Filling of a 96-well plate requires less than 10 seconds. Loading of 384-well plates is possible without any problems in only four pipetting steps. For the first time high-throughput experiments can be performed by avoiding high costs and saving staff resources.

High Accuracy and Safety

By using high quality Pipetman tips an accuracy and precision level equalling an 8- or 12- channel pipette is achieved in a single motion. Additionally, the risk of double-pipetting entire rows or columns is avoided.

A tailored fit

Through a variety of accessories such as adapters for loading 384-well plates, reagent reservoirs or sample heater blocks, the PlateMaster can be fully adapted to your needs.


For further information material please click here 


Contact:

Gilson International B.V. Deutschland

Hoenbergstr. 6 

65555 Limburg-Offheim 

Deutschland

Tel. +49 (06431) 21215-0

Fax +49 (06431) 21215-40

www.gilson.com 

Infors-HT

The Celltron has been ideally adapted for use in CO2-incubators which allows researchers an entry into shaken cell culture, where they can profit from considerably improved cultivation results.

Infors-HT

Your entry into shaken cell culture


Key Benefits:

  • Best results
  • Gentle magnetic drive
  • Antimicrobial surface
  • Minimal heat emission
  • Intuitive operation
  • Corrosion resistant
  • Maximum reliability


The Celltron has been ideally adapted for use in CO2-incubators. This allows researchers all over the world an entry into shaken cell culture, where they can profit from considerably improved cultivation results. Due to the minimal power consumption of only 4 watts, there is practically no heat dissipation into the incubator. This guarantees constant temperatures in the incubator and ideal conditions for cell culture.

The magnetic touch controller is affixed to the exterior of the incubator cabinet, allowing you to retain an overview of the operational status at all times. Visual and audible alarms are generated externally at the controller and can be immediately perceived – and that translates into reliability for your cells!

An additional plus for the cell culture user is the certified antimicrobial surface of the shaker, which reliably kills microorganisms.

For more information and your local sales office please visit: www.infors-ht.com

Celltron – Move your cells!


Contact:

Infors AG

Headoffice, Switzerland

Rittergasse 27

CH-4103 Bottmingen

T +41 (0)61 425 77 00

F +41 (0)61 425 77 01

headoffice@infors-ht.com

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