The Biometra GmbH, a company of the Analytik Jena AG, now offers illuminators with high-performance blue light LEDs for gel documentation purposes.
Blue light transilluminators are a quite interesting alternative to UV tables as there is no risk of sample damage during illumination. This is important when samples shall be processed furthermore after gel documentation. Users also benefit from it as there is no risk of UV exposure. Blue light excitation is applicable for fluorescent dyes for nucleic acid or protein stains with excitation wavelength around 470 nm. Examples for compatible stains are: SYBR® Green, GelGreenTM, SYBR® Safe, SYBR® Gold or SYPRO® Ruby. The amber lid of the instrument allows the direct view to the fluorescent samples. There is no need for separate glasses. The new blue light illuminators are available in two versions: "BLstar 9", the handy solution for mini gels and "BLstar 16" for gels up to size of 16 cm x 20 cm.
PeproGrow-1 is designed to be used as a supplement to serum-free cell culture media formulations.
PeproGrow-1 (Catalog # 700-C100) is designed to be used as a supplement to serum-free cell culture media formulations that can sustain the growth of adherent mammalian cell lines, and has been tested with HEK-293, HeLa, and A549 cells. This kit could potentially improve the culturing conditions of other adherent cells, but suitability for cells other than those pre-tested is not guaranteed. PeproGrow-1 is protein-free, has a chemically defined composition, and does not contain any animal-derived materials. It is intended specifically to be used with DMEM/F12 basal media (Invitrogen catalog # 10565*) and contains enough material to supplement 10L of media.
The PromoCell MSC Growth Medium DXF eliminates the risks of using traditional FCS-containing MSC media and this innovative formulation provides significant benefits.
Chemically defined and humanized culture of MSC
Undifferentiated Mesenchymal Stem Cells (MSC) can be expanded in vitro to produce of large numbers of cells. For this expansion, traditional MSC culture media that contains special pre-qualified lots of fetal calf serum has been necessary. In addition to the serious ethical concerns relating to the use of FCS, this ill-defined component of animal origin is the major obstacle in providing consistent culture conditions and optimal performance of MSC.
The PromoCell MSC Growth Medium DXF eliminates the risks of using traditional FCS-containing MSC media. This innovative formulation provides significant benefits:
· Chemically defined
· Humanized: animal-free / xeno-free
· No adaption of cells required
· Outstanding expansion performance
· Maintenance of multipotency
· Supports primary isolation
· Standardized: minimal lot-to-lot variation
· Cost effective: more cells with less medium
For economical serum-free routine culture of MSC, the MSC Growth Medium DXF may be used in combination with bovine fibronectin as an ECM coating and our Detach Kit. For chemically defined culture conditions, the trypsin-containing DetachKit should be replaced by the Cell Dissociation Solution ACF. The use of human fibronectin and the Cell Dissociation Solution ACF completes the MSC Growth Medium DXF to yield a powerful animal-free and chemically defined complete culture system.
The packing materials and packed columns are an ideal choice for high performance liquid chromatography.
Extensively used in laboratories all over the world, our TSKgel is designed for researchers seeking the highest level of performance. Covering the total range of HPLC, this product is made from small, spherical particles and offers high resolution (even at high flow rates), excellent reproducibility, and long column life. Scaling up from analytical to preparative columns is made simple and easy.
The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).
By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.
This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.
Currently kits are available to:
• quantify and qualify NGS fragments. • quantify and qualify RNA. • quantify and qualify genomic DNA. • do Mutation Detection, also known as TILLiNG, a reverse genetics technique.
The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.
Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.
An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.
ForteBio announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems.
ForteBio, a division of Pall Life Sciences and a leading supplier of instrumentation for accelerated drug discovery and development, today announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems. The Octet HTX system addresses the acute industry need for increased analysis speed and sample throughput when characterizing large numbers of therapeutic candidates.
Octet systems provide unique insights into drug-target binding interactions, insights that are used to fine-tune the affinity and specificity of drug molecules. The Octet HTX instrument performs kinetic analysis of up to 96 binding interactions simultaneously, a capability offered by no other stand-alone label-free platform. The system offers dramatic time savings for researchers studying large panels of drug candidates, and provides innovative data analysis tools for developers of monoclonal antibodies and other biotherapeutic entities. In addition, the Octet HTX performs full 96-well plate determinations of protein concentrations in as little as 2 minutes.
“The need for higher analytical throughput is a central theme with our pharmaceutical and biopharm customers,” noted Robert Wicke, General Manager at Pall’s ForteBio Division. “We believe researchers will be delighted by the Octet HTX system. The HTX system was engineered for speed while maintaining peak performance, and will directly address critical bottlenecks in the drug development process.”
The Octet HTX system was unveiled today at the BioProcess International 2013 conference in Boston, Mass.
Gel-free. Blot-free. Hands-free. A Simple Western is the Western you know well, only simplified.
Gel-free. Blot-free. Hands-free.
A Simple Western is the Western you know well, only simplified. It is not a workaround to the existing process, a Western-like substitute, or a partial solution. It's the real thing, completely reinvented. We at ProteinSimple think that's something to get excited about!
Get your time back. Load your samples in Simon, Sally or Peggy, press start and go enjoy your new hands-free time. Come back a few hours later to fully analyzed results. Get more consistency. People introduce variability—we arehuman after all. Simple Westerns are fully automated, so they just give you more reproducible data. Get truly quantitative. Since blot transfer of proteins isn't needed, you can get straight to real, quantitative results. Get more efficient. Sometimes you can only squeeze out enough sample for one experiment. Simple Westerns require a lot less protein and antibody so you have enough for those replicates.
• Learn about Simple Westerns • Learn about Peggy • Learn about Sally • Learn about Simon • Learn about NanoPro 1000 • Learn about Compass Software 21 CFR Part 11 Compliance • Simple Western Systems Comparison Chart
The Model 936 pneumatic pinch valve is a 2-way, compressed air or gas operated pinch valve that is designed to work with Shore A class flexible tubing.
Designed for bioprocessing systems applications such as single-use disposable (SSD), filling, mixing, dispensing, me- dia production and more
Supports user selectable tubing up to 1.5” Outer Diameter and 4 bar/58 psi media pressure
Built in user safety for finger crushing hazards for GLP/GMP
Available in 316L stainless steel or black anodized alumi- num material formats
The Model 936 pneumatic pinch valve is a 2-way, compressed air or gas operated pinch valve that is designed to work with Shore A class flexible tubing. Its robust design allows users the flexibility to select various types of biopharm or medical tubing within guideline specifications. The panel mount ready unit support tubing sizes from 1.0 to 1.5 inch outer diameter (25—38 mm), up to 65-Shore A and media pressures up to 58 psi/4 bar that allow for medium to large fluid volume movement. Built in tube loading and replace- ment safety lockouts ensures GLP/GMP compliancy preventing us- er finger or hand injury. Internal seals prevent liquid penetration inside the unit. Configured as either normally-open, or closed de- fault formats. Panel or base mountable and an optional state sen- sor is available.
Synergy™ Neo is an HTS multi-mode microplate reader designed specifically for today's screening and core laboratories.
Synergy Neo has all the features expected in an HTS screening instrument, including multiple parallel detectors for ultra-fast measurements and a dedicated filter-based system for live cell assays. With a load/unload plate transfer time of about 6 seconds per plate, Synergy Neo ’s optional integrated plate stacker is the fastest stacker on the market, allowing walk-away automation of short- or long-term assays. Synergy Neo also meets today's diverse assay requirements by incorporating features such as a powerful 100 mW laser-based excitation source for Alpha assays, a unique patented Hybrid optical design, advanced temperature control to 65°C, orbital and linear shaking. Ease of use and user confidence are key to the design of Synergy Neo ’s unique filter cubes that are barcode labeled for positive filter ID - limiting the possibility of errors and streamlining the workflow, while an available image-based barcode reader can scan both 1D and 2D barcode-labeled plates. The outstanding functionality of Synergy Neo is controlled by the powerful, yet easy to use, Gen5 2.0 Data Analysis Software. If you are involved in the HTS screening world, Synergy Neo was designed just for you.
Cambridge Epigenetix Ltd., a spin out biosciences company, has announced the launch of its innovative TrueMethyl™ Kits.
Available to order now via its website at www.cambridge-epigenetix.com, the kits represent a ground-breaking development for the epigenetics industry.
TrueMethyl™ brings unprecedented clarity to the analysis of DNA by providing quantitative, accurate and repeatable single-base resolution sequencing of the modified bases hydroxymethylcytosine (5-hmC) and methylcytosine (5-mC) for the first time. Early studies indicate that these modifications may have distinct and important physiological functions.
Traditional bisulfite sequencing cannot discriminate between 5-hmC and 5-mC. Recent studies have shown that at some sites in the genome the level of 5-hmC can be comparable to the level of 5-mC, emphasizing the importance of identifying these variants accurately. The high quality and easy to use TrueMethyl™ kits utilize innovative oxidative bisulfite sequencing (oxBS-Seq), a chemistry-based technology invented by Prof. Shankar Balasubramanian and Michael Booth from the University of Cambridge.
TrueMethyl™ kits can be used with a variety of common platforms including next generation sequencing systems, methylation arrays, and targeted assays. Trials of the kits conducted at leading research centers around the world have already begun to yield new insights into genome function and highlighted the advantages of TrueMethyl™ relative to traditional approaches.
Prof. Wolf Reik, group leader at the The Babraham Institute and the senior author of the recently published Cell Stem Cell paper which is the first published study using TrueMethyl™ said, “Employing chemical oxidation gives very high conversion efficiencies and minimises sequence context effects, giving results of unparalleled quality. Consequently, this pioneering analytical method opens new avenues for basic research, pharmaceutical discovery and diagnostics. This technology was pivotal in enabling us to accurately profile 5-hmC and 5-mC during demethylation of DNA.”
Olympus will exhibit its BX3 upright microscope systems and VS120 virtual slide scanner at ECP 2013.
Designed specifically to meet the needs of a range of pathology applications, the systems ensure robust diagnosis and discovery alongside ergonomic operation.
Enhancing the workflow of research and diagnostic centres alike, the latest range of Olympus pathology microscope systems and accessories will be on show at the 25th European Congress of Pathology (ECP) 2013, 31 August – 4 September in Lisbon, Portugal. Delegates can visit booth 14 to view the Olympus BX3 range of upright microscopes and the Olympus VS120 virtual slide scanner.
The BX3 range of upright microscope offers complete flexibility in pathology applications due to a modular design. Ergonomics is a priority, and each user can set up the microscope to match their posture, for both comfort and convenience. The range includes several models focused on either clinical or research microscopy, and delegates of ECP will have a chance to view the fully automated BX63, the research-focused BX53 and the BX43 clinical imaging system.
The BX63 delivers high performance automated microscopy for research applications, and the system on display at booth 14 will also feature the unique dual chip Olympus DP80 digital camera. Combining colour and monochrome chips within the same housing allows joint colour and fluorescent imaging in pathology applications, for example when combining histological staining with fluorescent dyes on the same sample. The BX3 range lends itself to easy integration into existing workflows, and visitors can also discover how the BX43 clinical microscope is compatible with both third party cameras and software.
With a modular design, the research-focused BX53 can be easily customised and the system on display at ECP will feature the Olympus discussion tube unit. Allowing multiple users to observe the same specimen using separate eyepieces, this system is ideal for both teaching and consulting, as all users see the actual image, not a digital representation. The unit is a powerful tool for reviewing difficult cases with other pathologists and colleagues to discuss diagnoses, and delegates of ECP are invited to bring along their own samples to examine with colleagues.
Also on display will be the Olympus VS120 virtual slide scanner, which features the flexibility, resolution and image quality necessary for meeting the demands of pathology research workflows. The system creates a “virtual slide” high-resolution image of the complete specimen at a range of magnifications, which can be electronically stored on a central server. This enables instant access and simultaneous viewing anywhere in the world, omitting the need to transport specimens between laboratories, reducing slide archives and securing a permanent digital record of the sample.
Delegates will discover how the latest in Olympus imaging technology enables new possibilities for clinical and research pathology applications alike.
7th Berlin Conference on IP in Life Sciences: Big Data, Big Drugs
The health care industry faces significant transformation, driven by a boom in knowledge within biomedical sciences and breakthrough technologies such as gene sequencing. The management of "big data“ will change the understanding of diseases, development of drugs and treatment of patients. more