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Laser Option for New Gallios Flow Cytometers
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Beckman Coulter Life Sciences

Laser Option for New Gallios Flow Cytometers

A 561 nm laser option, available for new 561 nm-laser ready Gallios* flow cytometers from Beckman Coulter Life Sciences, will be on view at CYTO 2012, June 23-27 in booth 319 at the Congress Center in Leipzig, Germany. The 561 nm laser option provides researchers with the ability to analyze a greater range of fluorescent proteins and to more efficiently excite PE/PE tandems. Optimal excitation is offered for red fluorescent proteins such as mCherry and DsRed, which are being increasingly used in research labs.

Yellow-green excitation provided at 561 nm produces less autofluorescence in cells, increasing the signal-to-noise ratio for PE and DsRed detection.  Since 561 nm excitation is well separated from fluorescein and GFP emission, there is virtually no crossover contamination in the green detection channel, allowing simultaneous fluorescein, or GFP detection, without blocking optics.

The new laser is externally coupled through an optical fiber and can be field installed on new Gallios 561 nm-laser ready analyzers.  The 561 nm laser will be passed to the flow cell collinearly with the 488 nm laser during acquisition.

Available configurations for the Beckman Coulter Life Sciences’ Gallios flow cytometers are: 6 colour/2 laser, 8 colour/2 laser, 10 colour/3 laser and 10 colour/4 laser – the last configuration reflecting the 561 nm option. Six fluorescence detectors provide simultaneous acquisition of up to six fluorescence signals. Concurrent measurements of integral, peak and width are available for all parameters, including scatter and fluorescence signals.

“Expanding the colour options on the Gallios cytometer will extend the applications of the instrument for research scientists,” said Mario Koksch, director of project management and strategy at Beckman Coulter Life Sciences.  “Researchers will be able to further refine their studies using the full capabilities of the cytometer.”

Gallios cytometers feature an innovative forward-scatter detector that provides up to three measurements of cell size and visualization of particles down to 0.404 mm in diameter.  A side-scatter detector incorporates an independently focused, high-performance photodiode with electronic attenuation. A selection of up to 62 parameters can be processed per analysis, at acquisition rates of 25,000 events-per-second, with high yield.  Easily interchanged optical filters facilitate detection of a variety of dyes and wavelengths.


For more information: 

Beckman Coulter Life Sciences

Hillary Hunter, Public Relations

Tel: +1 714 961 5620

hillaryhunter[at]beckman.com

www.beckmancoulter.com

BD

With 100g samples, the Peptone Starter Paks allow for you to identify the correct supplements for your specific cell line faster.

BD Biosciences

BD is helping you rethink supplementation. For more than 100 years, leading pharmaceutical companies have trusted BD peptones and supplements to help improve cell culture performance with higher cell titers and yield.

The new BD Peptone Starter Paks provide samples of our most widely used peptones tailored for specific applications, including monoclonal antibodies, recombinant proteins, and vaccines, for use in both mammalian cell culture and microbial fermentation.

With 100g samples conveniently packed in one box, you can identify the correct supplements for your specific cell line faster.

Request a starter pak today and start maximizing your cell’s performance. Go to www.bdbiosciences.com/go/peptone.

Peptone Starter Paks are for evaluation use only.


Contact:

BD

7 Loveton Circle

Sparks, MD 21152

bdbiosciences.com/advbio

Pall

The Cadence Inline Concentrator simplifies concentrating solutions of biomolecules to an in-line, single-use, ready-to-use process.

PALL

The recirculation loop associated with tangential flow filtration (TFF) conventionally used for this process is eliminated. Pump and tube sizes are reduced, product recovery is maximized, and sensitive proteins are exposed to less shear. The Cadence Single-Use Inline Concentrator moves the product being processed through a proprietary design flow path in a pre-assembled module with all connections already in place and designed to couple to available standard tubing manifolds.

Typical applications include monoclonal antibodies, fractionated antibodies, recombinant proteins, other proteins and recombinant blood factors. The Cadence Single-Use Inline Concentrator is used to concentrate clarified bulk after fermentation, and to reduce the number or size of bags needed and increase product concentration to improve chromatography performance. 

Cost projections with BioSolve software show that, compared to conventional single-use TFF, the Cadence Single-Use Inline Concentrator provides a 31% cost of goods sold and a 66% capital investment savings per batch because of the elimination of the need for intermediate tanks, ability to use smaller chromatography columns, energy savings, and the ability to couple processes to reduce downtime.


For further information please click here.

ProteinSimple

Gel-free. Blot-free. Hands-free. A Simple Western is the Western you know well, only simplified.

ProteinSimple

Gel-free. Blot-free. Hands-free.

A Simple Western is the Western you know well, only simplified. It is not a workaround to the existing process, a Western-like substitute, or a partial solution. It's the real thing, completely reinvented. We at ProteinSimple think that's something to get excited about!

Get your time back. Load your samples in Simon, Sally or Peggy, press start and go enjoy your new hands-free time. Come back a few hours later to fully analyzed results.
Get more consistency. People introduce variability—we arehuman after all. Simple Westerns are fully automated, so they just give you more reproducible data.
Get truly quantitative. Since blot transfer of proteins isn't needed, you can get straight to real, quantitative results.
Get more efficient. Sometimes you can only squeeze out enough sample for one experiment. Simple Westerns require a lot less protein and antibody so you have enough for those replicates.


• Learn about Simple Westerns
• Learn about Peggy
• Learn about Sally
• Learn about Simon
• Learn about NanoPro 1000
• Learn about Compass Software 21 CFR Part 11 Compliance
• Simple Western Systems Comparison Chart

Contact:

Dr. Markus Goddemeier

Sales Specialist

T: +49 (0)761 477 07 63

M: +49 (0)162 985 79 53

www.proteinsimple.com

Lee Products

Miniature component specialists LEE PRODUCTS have recently published a new EFS (Electro Fluidic Systems) technical handbook which provides a valuable resource of product information along with practical engineering reference material.

Lee Products

Lee Product’s three Electro-Fluidic Systems groups produce high quality miniature solenoid valves, micro-dispense valves, atomising and dispense nozzles, fixed and variable volume pumps, integrated fluidic manifolds, inert tubing and fluid control components and custom engineered designs.  These products are used in medical and scientific instrumentation, analytical / clinical chemistry, in-vitro diagnostics, drug discovery and ink jet printing applications.

This new 323 page EFS handbook includes detailed information on Lee’s wide range of solenoid valves and pumps along with their manifolds, tubing, accessories, and special products, plus useful engineering and general information.


More details and copies of the handbook are available from:

Mike Stott

Lee Products Ltd

3 High Street

Chalfont St. Peter

Gerrards Cross

Bucks, SL9 9QE

Tel: +44 (0) 1753 886664

Fax:+44 (0)1753 889588

sales[at]leeproducts.co.uk

www.leeproducts.co.uk

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package for parallel bioprocess control, which brings a fresh new look and a whole raft of new features.

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package forparallel bioprocess control. Iris 6 brings a fresh new look and a whole raft of new features to one of themost flexible and user friendly bioprocess control software programs available today. A special versionfor use with Multitron incubation shakers allows comprehensive process validation.

“Beginners will love the modern design and the easy navigation”, says Dr. Tony Allman, productmanager INFORS HT. A new ribbon menu provides quick access to the bioreactor and parametersettings. A variety of graphical displays such as correlation and bar graphs allow an easy interpretationof process data. The new version allows the user to annotate on-line graphics while a fermentation isin progress and to time-stamp samples for easy reference.

Advanced features such as the feedback control using sequences allow the experienced user toexplore all possibilities of bioprocess control. The seamless integration of both analysed and calculatedprocess parameters gives the users the possibility to achieve a combined record of all process data.“The implementation of off-line analysed parameters like OD or protein yield in the batch record file isgreat, now I can record all data in one single file”, says Dr. Michael Kaempf, Project Leader BioprocessDevelopment, EMPA (Switzerland). An enhanced reporter module makes creation of batch tickets, data export and printing both comprehensive and simple.

The Iris Parallel Bioprocess Control Software is available in 3 different packages depending on the type of equipment. The same software provides high quality data acquisition and sophisticated controlstrategies from shake flask up to pilot production.

All software packages can be qualified for validation purposes relating to FDA CFR Part 11, 21.


Contact:

Dr. Tony Allman, Product Manager Fermentation

Infors AG

Rittergasse 27

4103 Bottmingen

Switzerland

Phone: +41 (0)61 425 77 00

Fax: +41 (0)61 425 77 01

e-Mail: t.allman[at]infors-ht.com

Web: www.infors-ht.com

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.  The analyte specific reagents (ASRs) provide high specificity, convenience and flexibility in flow cytometry.  A new cell permeabilization reagent is also being offered. 

The new monoclonals include human anti-hemoglobin F, which recognizes a fetal hemogloblobin molecule; CD163 markers for anti-inflammatory signal and disease process research and CD64 markers, which react with Fc gamma receptors. Characterized, quality controlled, convenient, liquid reagents allow users to spend less time in sample preparation and more time on experiments.

Rounding out the line is a monoclonal for MNDA (myeloid nuclear differentiation antigen). All antibodies are available unconjugated or conjugated to a selection of fluorochromes. Intra-Cell™ concentrate for cell permeabilization is also being released.

Beckman Coulter Life Sciences offers an extensive line of human and non-human antibodies as well as a range of supporting reagents and immune monitoring products. The new antibodies are supplied in 1.0 mL of buffer, are ready to use and facilitate flexibility in experimental design.

“Convenience and specificity are important features for scientists,” said Mario Koksch, vice president and general manager for Beckman Coulter Life Sciences’ Flow Cytometry Business Unit. “These new antibodies are directed at important targets and will improve accuracy for researchers.”

A strategic partnership between the companies enables Beckman Coulter Life Sciences to further diversify their extensive flow cytometry reagent portfolio and Trillium Diagnostics to broaden the reach of their products in the global market.    


Contact Information:

Hillary Hunter

Beckman Coulter Life Sciences Public Relations

hillarythunter[at]beckman.com

www.beckmancoulter.com

Advanced Analytical Technologies

The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).

Advanced Analytical Technologies

By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.

This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.

Currently kits are available to:

• quantify and qualify NGS fragments.
• quantify and qualify RNA.
• quantify and qualify genomic DNA.
• do Mutation Detection, also known as TILLiNG, a reverse genetics technique.

The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.

Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.

An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.

Contact:

Advanced Analytical Technologies GmbH

Lutz Büchner

Im Neuenheimer Feld 583

69120 Heidelberg

Tel.: +49-(0)-6221-868058-20

lbuechner[at]aati-de.com

www.aati-de.com 

GE Healthcare

His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.

GE Healthcare

Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells. 

Key features:

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several different formats for screening and preparative purification of histidine-tagged proteins 

Background:

IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.


Contact Information:

GE Healthcare Europe GmbH

Life Sciences

Andrea Löhndorf, PhD, MBA

Regional Marketing Manager

Mobil: +49 (0)174 92 36 400

andrea.loehndorf[at]ge.com

www.gelifesciences.com

SARSTEDT

Sarstedt offers an extensive range of certified and geometrically optimised pipette tips with and without filters for all common pipettes.

Sarstedt

The hydrophobic filter material prevents the aerosol, which is formed when pipetting, from contaminating the pipette. Our pipette tips are characterised by their high transparency and accuracy. Compliance according to DIN EN ISO 8655 is tested with the most common pipettes. The pipette tips are available in a bag, box or economical refill system, in three purity grades and as a low retention version.

For further information please click here.

CETICS

AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.

CETICS Healthcare Technologies GmbH

In contrast to conventional in-vitro diagnostic methods,  AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.

The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.

Fields of application:

  • Toxicology
  • Cell Culture Quality Control
  • Pharmaceutical Development


Ask us for additional information:  

CETICS Healthcare Technologies GmbH

Schelztorstr. 54-56

73728 Esslingen am Neckar

Germany

Phone:+49 (0)711 300 655-0

Fax:+49 (0)711 300 655-29

e-mail: info[at]cetics-ht.com

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