The ImagEM X2, a completely redesigned camera featuring a back-thinned EM-CCD sensor, offers maximum speed and precision performance for low-light imaging.
The ImagEM X2 makes superfast exposures possible and has the sensitivity to provide visually pleasing and quantitatively meaningful images in a photon-starved environment. It delivers 70 frames/s at the full resolution of 512 x 512 pixels with a high signal-to-noise ratio, enabling quantitative high-speed, low-light imaging. When binning or a region of interest is selected, this new camera produces images at even higher frame rates (up to 1076 frames/s).
Additional features allow for optimized camera triggering and streamlined connectivity through IEEE 1394b. The ImagEM X2 also has improved overall signal-to-noise ratio, increased non-EM dynamic range, and built-in EM gain measurement and calibration functions. It also features a software-controllable shutter that prevents EM gain degradation and image lag. To prevent EM gain degradation, the shutter is closed when the built-in EM gain protection feature is enabled. In addition, closing the shutter when a user replaces a lens, for example, can prevent image lag.
The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards.
The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the µDAWN is similar to the well-established miniDAWN™ TREOS™, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.
Distinctly from the miniDAWN, the µDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the µDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.
The µDAWN may also be used in microbatch (off-line) mode or in conjunction with a Calypso IICG-MALS system for characterization of macromolecular interactions: affinity and absolute stoichiometry, label-free and immobilization-free, as well as second virial coefficients, cross virial coefficients, average molar masses and sizes.
GE Healthcare launches Cytell™ Cell Imaging System for compact, app-driven, automated imaging - Streamlines data acquisition, analysis and visualisation
Cytell™ Cell Imaging System is an intuitive benchtop instrument equipped with on-board data analysis and visualisation tools. It streamlines and simplifies routine assays such as cell cycle and cell viability and efficiently delivers high quality, quantitative results.
Key to the simplification are the on-board Bioapps; pre-configured, easy to use software modules that automate all the steps from imaging and data acquisition through data analysis, visualisation and report generation. The Cytell cell imaging system is compact and small enough to sit on a benchtop.
Cytell can image a wide variety of sample formats including slides, petri dishes, T-flasks and microplates. Compatible GE Healthcare Life Sciences reagents and kits are available, although Cytell is an open system and therefore provides the flexibility to create customized protocols based on other suppliers’ reagents.
BioApps currently available include:
Digital Imaging BioApp: Acquire fluorescence and transmitted light images of samples in plates, flasks, Petri dishes, or slides.
Automated Imaging BioApp: Automatically acquire high-quality fluorescence and transmitted light images from an entire multiwell plate in minutes.
Quick Count BioApp: Obtain accurate cell counts and viability and concentration estimations based on fluorescence intensity using a disposable hemocytometer.
Cell Cycle BioApp: Analyze cell cycle phase distribution in multi-well format using a DNA-binding fluorophore.
Cell Viability BioApp: Run two- or three-color assays in multiwell formats with subpopulation gating to determine the percentages of live, dead, and non-viable cells.
With improvements to the product and above all with enhanced documentation, the new minilab presents a huge number of new research opportunities for any user.
A new edition of the "lab in a suitcase"
For many years the Zinsser minilab has been an important component as a teaching aid in chemistry lessons in schools and universities. Now, with improvements to the product and above all with enhanced documentation, the new minilab presents a huge number of new research opportunities for any user.
Even though in the modern fine chemical industry and in pharmaceutical research and development where the smallest possible sample and reagent volumes are used (from a few mls and µls), for practical teaching, the experimentation often takes place in the front of the whole lesson with the use of larger volumes - up to 100mls - in schools and universities.
Containing an innovative set of glass equipment, the Mini Lab „lab in a suitcase“ is now used in many schools in different countries. There is now a new manual available for Mini Lab which includes more than 40 experiments - from basic operations such as distillation, filtration etc to complex syntheses such as colorants as designed by Prof. Michael Schallies. The instructions for the equipment configuration are easy to understand and are explained by simple drawings. General tips for the implementation of single experiments, material notes as well as danger symbols for the reagents are also included in the test description. The expected experimental results are also described in detail and partly documented with pictures or graphics. In the appendix of the manual there are detailed explanations of the potential dangers and also safety tips.
Current Owners of the Mini Lab can have a new updated manual for a small nominal charge.
Zinsser Analytic GmbH develops and manufactures in Germany and supplies customized automation technology and laboratory accessories for the global market. The company has specialized in the supply of high end technological products and systems for automated analysis in the fine chemical industry and also biological research and development. The company has grown over the years reaching a turnover in the range of double-digit millions and has about 60 employees to date.
Sartorius Stedim Biotech, a leading global supplier of the biopharmaceutical industry, is introducing a brand-new, scalable range of single-use bags.
Its completely new developed product family Flexsafe enables theimplementation of single-use bioprocessing throughout all steps of drug manufacture, fromprocess development to production, in upstream and downstream – all using just the oneinnovative polyethylene film. The innovative concept of the Flexsafe family addresses key industryrequirements for future-proof single-use manufacturing of commercial vaccines and drugs.
Flexsafe is based on a multilayer, proprietary polyethylene (PE) film, called S80, and has beendeveloped in close collaboration with resin and film suppliers. A standardized cell growthassay has been used to optimize film formulation, determine the operating ranges for extrusion,welding and gamma-irradiation processes and to establish specifications and process controls.
Flexsafe ensures excellent and reproducible cell growth behavior of the most sensitive celllines. The optimization of the resin formulation, the complete control of raw materials, theextrusion process and the bag assembly guarantee lot-to-lot consistent cell growthperformance. With its robust 400 μm thick PE film, Flexsafe is the strongest and most flexiblebag currently on the market. The bags enable safe and easy-to-use operation, even in themost demanding applications such as liquid shipping and large-scale stirred bioreactors.
Furthermore, batch-to-batch consistent extractables and leachables profiles support drugmanufacturers throughout the entire lifecycle of modern biological treatments from clinicaldevelopment to commercial supply many years after launch. Users can gain assurance thattheir initial extractable and leachable qualification work and data remain valid every timethey operate their single-use Flexsafe bioprocess.
Assurance of supply, one of the most important aspects for manufacturers, is guaranteed as aresult of long-term contracts with suppliers and business continuity plans with defined safetystocks and global manufacturing capabilities including resin manufacturing.
„Flexsafe represents a completely new generation of single-use bags. They offer our customersconsistent cell growth as well as extraordinary robustness and flexibility. In addition asustainable supply chain and consistent film throughout the entire manufacturing process isguaranteed. Every single one of these benefits sets a new benchmark. Being unique in all ofthem makes us very proud”, stated Stefan Schlack, Senior Vice President Marketing andProduct Management at Sartorius Stedim Biotech.
Sartorius Stedim Biotech is launching Flexsafe RM bags (1L-200L) and small bags for validationpurposes first. Bags for single use bioreactors BIOSTAT STR (50-2000L) and additional applications such as storage, mixing, shipping freeze and thaw will be rolled out step by step.
AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.
In contrast to conventional in-vitro diagnostic methods, AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.
The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.
The ProdX software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient.
Seamless Integration of Product Inspection Devices
Whatever your role in product inspection management, you will have plenty of challenges to face in marketplaces that increasingly demand more for less.
ProdX Inspect data management software provides seamless integration of product inspection devices making processes more streamlined, more accessible and more efficient. ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
ProdX Savings Calculator
Calculate your time savings potential with ProdX
This Calculator runs through all costs involved in the setup and data collection processes. It calculates the amount you could save per year by comparing the time requirement of manual processes and the automated data collection process this software offers.
ProdX Quality Management & AssuranceThe innovative ProdX software automatically collects the critical inspection process data you need in one convenient location significantly reducing the need to interact directly with the Critical Control Points on your factory floor. This saves time and effort enabling quality operatives to focus on other issues.
ProdX Production Process Managment
ProdX allows you to control your entire product inspection management process from a single point or from multiple remote locations, eliminating the need for time-consuming production line patrols.
PromoKine offers a wide range of products for cell analysis including numerous kits for fast, convenient and sensitive measurement of cell viability/proliferation, cytotoxicity, cell death and apoptosis, cell stress and metabolism as well as reporter gene expression.
PromoKine’s Fluorometric Cell Viability Kits are based on resazurin or calcein-AM conversion while our Colorimetric Cell Viability Kits utilize reduction of different tetrazolium salts (e.g. MTT, XTT, WST-1 & WST-8), and the Bioluminescent Cell Viability Kits measure ATP levels as a marker for cell viability in a luciferase assay.
Our Cytotoxicity Kits quantify the amount of lactate dehydrogenase (LDH) or adenylate kinase (AK) released from damaged cells either colorimetrically or by using a luminometer.
PromoKine’s Apoptosis Assays include various kits for detection and quantitation of e.g., caspase activity, annexin V binding, DNA fragmentation or mitochondrial changes. Moreover, PromoKine provides Reporter Assay Kits (beta-galactosidase, luciferase, GFP) as well as a selection of small-sized reporter plasmids and fusion plasmids (target gene fused with GFP or luciferase gene).
In addition, you will find in our catalog and on our website a wide range of other interesting products for cell biology research such as numerous high-quality and economically priced recombinant proteins (e.g. caspases, kinases, cytokines, chemokines & growth factors), a variety of primary and secondary antibodies and ELISA kits as well as premium, user-friendly reagents and kits for optimal cell transfection, fluorescent staining of cell structures, labeling of biomolecules (proteins, antibodies and nucleic acids) and the detection, elimination and prevention of mycoplasma and bacterial contamination in cell culture.
ReadyTector is an all-in-one detection solution for Western blotting.
All-in-one solution for Western blots
The one-step solution contains all ingredients for quick immunodetection. Only a specific primary antibody has to be added. “All-in-one” stands for all ingredients in one solution as well as for all-in-one step. Blocking and binding of primary and secondary antibodies are carried out simultaneously.
The only additional step is washing with the specific ReadyTector Wash Buffer.
ReadyTector for Western blotting is easy, quick and clear:
# easy – very simple procedure
# quick – very fast procedure with very quick results
# clear – no background
The ReadyTector kit contains the all-in-one Solution and the Wash Buffer. Bottle sizes of 40 ml, 120 ml and 500 ml are available.
The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes.
1. Principle of Operation
The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes. The sample is passed through the membrane by pushing the sample back and forth between two syringes.
2. Temperature Control
The entire LiposoFast-Basic can be immersed in a water bath for use with high transition temperature lipids or heat sensitive compounds.
The optional LiposoFast-Stabilizer was designed to simplify the repetitive use of the LiposoFast-Basic as well as the extrusion of highly concentrated samples. It accommodates both 0.5mL and 1.0mL syringes.
4. Free Trial
This instrument is available for a free trial. Simply contact us and we can send it to you. If after the trial you are not satisfied with it or it does not suit your research, simply send it back.
Today, embryonic stem cells (ESC) and induced pluripotent stem cells (iPS) represent the most significant types of pluripotent stem cells (PSC).
In the beginning, ESC were cultured on feeder cell layers, primarily murine embryonic fibroblasts (MEF), in non-defined culture media containing fetal calf serum (FCS). Later on, FCS would be replaced by somewhat more defined serum replacements and the introduction of extracellular matrix (ECM) coatings supporting self-renewal of ESC allowed for feeder-free culture using feeder-cell conditioned medium. However, the culture process remained time-consuming, laborious and poorly defined.
In recent years PSC-culture has experienced significant technical progress. Chemically defined media compositions became a standard, followed by the first xeno-free defined formulations. However, all these culture systems still depended on non-defined, xenogenic and poorly standardized ECM preparations – a contradiction in terms when using defined / xeno-free media. Importantly, research recently overcame this hurdle, identifying natural as well as synthetic ECM molecules and peptides that support undifferentiated PSC-expansion. Scientists are now capable of completely defined / humanized derivation and culture of hPSC.
Despite these recent technical advancements, the established hPSC culture systems still share unfavourable properties. Most of them use supra-physiologically high amounts of growth factors and / or contain substances purified from human or animal origin. In addition animal-derived and / or non-defined ECM is used. The PromoCell hPSC Growth Medium DXF consequently eliminates these disadvantages:
Chemically defined / xeno-free complete culture system
No human / animal derived components
Physiologically low in growth factors
This optimal culture environment allows for a well-controlled culture process, consistent and reproducible performance, robust support of pluripotency and improved cloning efficiency.
In combination with the extracellular matrix hPSC-ECM DXF and the hPSC Dissociation Buffer DXF, PromoCell provides a defined and xeno-free complete culture system for human pluripotent stem cells.
The Innovative Medicines Initiative Joint Undertaking (IMI JU) is a public-private partnership between the European Commission and the European Federation of Pharmaceutical Industries Associations (EFPIA). The Executive Director will lead and manage the Joint Undertaking and take overall responsibility for its operations ensuring the achievement of the Joint Undertaking's objectives. more...
The First Multi-Angle Light Scattering Detector for UHPLC!
The µDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. more