Product of the week

His Mag Sepharose™ Ni

GE Healthcare

His Mag Sepharose™ Ni

His Mag Sepharose Ni products are magnetic beads designed for efficient, small-scale purification/screening of histidine-tagged proteins from different sources. Histidine-tagged proteins are captured using immobilized nickel ions followed by collection of the beads using a magnetic device. You can use magnetic beads to simplify sample handling during small-scale purification.

His Mag Sepharose Ni delivers:

  • High-capacity small-scale purification of histidine-tagged proteins from different sources
  • High purity and yield
  • Easy parallel screening of histidine-tagged proteins with high repeatability
  • Scalability—Simple capture of histidine-tagged proteins from small or large sample volumes (low microliter to milliliter scale)

The beads are available in three pack sizes: 2 × 1 ml 5% medium slurry, 5 × 1 ml 5% medium slurry, and 10 × 1 ml 5% medium slurry—a 1 ml 5% medium slurry is the same as 50 μl sedimented medium. A 1 ml medium slurry is sufficient for 5 purification runs according to the recommended protocol.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device.

Simplified handling:

The magnetic bead format has excellent properties for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins in the purification procedure. The characteristics of the media are summarized in Table 1. All the products are provided with protocols optimized for purification of histidine-tagged proteins.

Tabel 1. Characteristics of His Mag Sepharose Ni

  • Matrix: Highly crosslinked spherical agarose (Sepharose) including magnetite
  • Metal icon capacity: 21µmol Ni2+/ml medium
  • Binding capacity: approx. 50mg histidine-tagged protein/ml medium (approx. 500µg/purification run)
  • Particle size: 37 to 100µm
  • Working temperature: Room temperature and + 4ºC
  • Storage solution: 20% ethanol, 5% medium slurry
  • Storage temperature: Room temperature



GE Healthcare Europe GmbH

Dr. Michael Walther
EMEA Marketing Manager Electrophoresis

T    + 49 2293 816 749
M   + 49 173 574 9413
F    + 49 5564 204 593

Lee Products

Miniature component specialists LEE PRODUCTS have recently published a new EFS (Electro Fluidic Systems) technical handbook which provides a valuable resource of product information along with practical engineering reference material.

Lee Products

Lee Product’s three Electro-Fluidic Systems groups produce high quality miniature solenoid valves, micro-dispense valves, atomising and dispense nozzles, fixed and variable volume pumps, integrated fluidic manifolds, inert tubing and fluid control components and custom engineered designs.  These products are used in medical and scientific instrumentation, analytical / clinical chemistry, in-vitro diagnostics, drug discovery and ink jet printing applications.

This new 323 page EFS handbook includes detailed information on Lee’s wide range of solenoid valves and pumps along with their manifolds, tubing, accessories, and special products, plus useful engineering and general information.

More details and copies of the handbook are available from:

Mike Stott

Lee Products Ltd

3 High Street

Chalfont St. Peter

Gerrards Cross

Bucks, SL9 9QE

Tel: +44 (0) 1753 886664

Fax:+44 (0)1753 889588


With Iris 6 INFORS HT releases a new SCADA software package for parallel bioprocess control, which brings a fresh new look and a whole raft of new features.


With Iris 6 INFORS HT releases a new SCADA software package forparallel bioprocess control. Iris 6 brings a fresh new look and a whole raft of new features to one of themost flexible and user friendly bioprocess control software programs available today. A special versionfor use with Multitron incubation shakers allows comprehensive process validation.

“Beginners will love the modern design and the easy navigation”, says Dr. Tony Allman, productmanager INFORS HT. A new ribbon menu provides quick access to the bioreactor and parametersettings. A variety of graphical displays such as correlation and bar graphs allow an easy interpretationof process data. The new version allows the user to annotate on-line graphics while a fermentation isin progress and to time-stamp samples for easy reference.

Advanced features such as the feedback control using sequences allow the experienced user toexplore all possibilities of bioprocess control. The seamless integration of both analysed and calculatedprocess parameters gives the users the possibility to achieve a combined record of all process data.“The implementation of off-line analysed parameters like OD or protein yield in the batch record file isgreat, now I can record all data in one single file”, says Dr. Michael Kaempf, Project Leader BioprocessDevelopment, EMPA (Switzerland). An enhanced reporter module makes creation of batch tickets, data export and printing both comprehensive and simple.

The Iris Parallel Bioprocess Control Software is available in 3 different packages depending on the type of equipment. The same software provides high quality data acquisition and sophisticated controlstrategies from shake flask up to pilot production.

All software packages can be qualified for validation purposes relating to FDA CFR Part 11, 21.


Dr. Tony Allman, Product Manager Fermentation

Infors AG

Rittergasse 27

4103 Bottmingen


Phone: +41 (0)61 425 77 00

Fax: +41 (0)61 425 77 01



Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.  The analyte specific reagents (ASRs) provide high specificity, convenience and flexibility in flow cytometry.  A new cell permeabilization reagent is also being offered. 

The new monoclonals include human anti-hemoglobin F, which recognizes a fetal hemogloblobin molecule; CD163 markers for anti-inflammatory signal and disease process research and CD64 markers, which react with Fc gamma receptors. Characterized, quality controlled, convenient, liquid reagents allow users to spend less time in sample preparation and more time on experiments.

Rounding out the line is a monoclonal for MNDA (myeloid nuclear differentiation antigen). All antibodies are available unconjugated or conjugated to a selection of fluorochromes. Intra-Cell™ concentrate for cell permeabilization is also being released.

Beckman Coulter Life Sciences offers an extensive line of human and non-human antibodies as well as a range of supporting reagents and immune monitoring products. The new antibodies are supplied in 1.0 mL of buffer, are ready to use and facilitate flexibility in experimental design.

“Convenience and specificity are important features for scientists,” said Mario Koksch, vice president and general manager for Beckman Coulter Life Sciences’ Flow Cytometry Business Unit. “These new antibodies are directed at important targets and will improve accuracy for researchers.”

A strategic partnership between the companies enables Beckman Coulter Life Sciences to further diversify their extensive flow cytometry reagent portfolio and Trillium Diagnostics to broaden the reach of their products in the global market.    

Contact Information:

Hillary Hunter

Beckman Coulter Life Sciences Public Relations

Advanced Analytical Technologies

The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).

Advanced Analytical Technologies

By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.

This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.

Currently kits are available to:

• quantify and qualify NGS fragments.
• quantify and qualify RNA.
• quantify and qualify genomic DNA.
• do Mutation Detection, also known as TILLiNG, a reverse genetics technique.

The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.

Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.

An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.


Advanced Analytical Technologies GmbH

Lutz Büchner

Im Neuenheimer Feld 583

69120 Heidelberg

Tel.: +49-(0)-6221-868058-20 

GE Healthcare

His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.

GE Healthcare

Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells. 

Key features:

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several different formats for screening and preparative purification of histidine-tagged proteins 


IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.

Contact Information:

GE Healthcare Europe GmbH

Life Sciences

Andrea Löhndorf, PhD, MBA

Regional Marketing Manager

Mobil: +49 (0)174 92 36 400


AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.

CETICS Healthcare Technologies GmbH

In contrast to conventional in-vitro diagnostic methods,  AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.

The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.

Fields of application:

  • Toxicology
  • Cell Culture Quality Control
  • Pharmaceutical Development

Ask us for additional information:  

CETICS Healthcare Technologies GmbH

Schelztorstr. 54-56

73728 Esslingen am Neckar


Phone:+49 (0)711 300 655-0

Fax:+49 (0)711 300 655-29



Cytation 3 is an automated digital microscope that provides high quality cellular imaging up to 60x in slides, microplates, Petri dishes and cell culture flasks.


Cytation™ 3 cell imaging multi-mode system combines automated digital microscopy and conventional multi-mode microplate detection in one instrument. This unique patent pending design provides rich phenotypic cellular information with well-based quantitative data, bringing cell biology research to a new level.

Fully integrated solution for automated fluorescence and brightfield microscopy of slides, microplates and flasksTemperature control to 45°C, CO2/O2 gas control and orbital shakingPowerful yet easy to use software for efficient image capture and analysisEliminates inconveniences commonly associated with conventional microscopy

The modular platform can easily upgrade to add conventional multi-mode microplate detection, to provide well-based intensity data along with phenotypic information from the microscope.


This is a highly advanced programmable pneumatic digital dispenser. It can store eight programmes and it can partially compensate for viscosity variation.


The dispenser features new digital controller technology with a clear digital display for a simultaneous overview of all parameters.

The Drifton 206-DF dispenser can store eight programmes in the controller’s memory and when it is programmed, it will adjust the pneumatic pressure automatically as the liquid becomes thicker or thinner.

The dispenser can partially compensate for variation in the dispensed medium.

Remember to order an air fitting to go with this one-component dispenser.


Drifton A/S
Avedøreholmen 84
DK-2650 Hvidovre
Tel.: +45 3679 0000

Life Technologies

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs.

Life Technologies

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. SuperScript® III RT features:

  • Thermostability—half-life of 220 min at 50°C
  • Yield—reduced RNase H activity for more full-length cDNA
  • Specificity—full activity at 50°C for increased specificity with gene-specific primers
  • Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3´ and 5´ RACE

For further information please click here.

Porvair Sciences Ltd

Porvair Sciences will present its complete range of automation friendly microplates and equipment at European Lab Automation 2012 in Hamburg, Germany in partnership with long-term collaborator, KBiosystems.

Porvair Sciences Ltd

Visitors to the Porvair / KBio stand at ELA 2012 will be able to see the Universal Robotic Manifold plus a growing range of high quality reagent reservoirs. The Universal Robotic Manifold is designed to be easily assembled and disassembled by robotic manipulators, thus enabling productive automation of SPE or DNA clean-up procedures. Able to accommodate collection plates from 14mm - 44mm in height and adaptable for working with short, medium and long skirts and drip directors, the Universal Robotic Manifold can operate with many different brands of filter plate and collection trays. Supplied with an integral vacuum gauge, the Manifold provides complete control of vacuum pressure ensuring plates can be processed with high reproducibility.

Combining uncompromising high quality with an affordable price - Porvair Sciences will display its extensive range of deep well, shallow well, storage, assay, SPE, protein precipitation and filtration microplates precisely manufactured to comply with ANSI/SBS dimensions ensuring complete compatibility with all automated sample handling systems, microplate readers and washers. 

Also on display will be the Ultravap RC - Porvair's all new robot-compatible nitrogen blow down station. For many years, Porvair Sciences has led the field in nitrogen blow down evaporation. This new version of Porvair's most popular sample concentrator makes it even easier to integrate an automatic dry down step into your automated liquid handling protocol.


Porvair Sciences Ltd

Dorset House, Regent Park

Kingston Road, Leatherhead

Surrey KT22 7PL  UK

Tel: +44-1372-824290

Fax: +44-1372-824271

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