Highly efficient transfection of primary cells and cell lines
PromoFectin provides highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells. It consists of a non-liposomal polymer that is stable, water-soluble and free of any components of animal origin. PromoFectin shows very low toxicity and is therefore ideally suited for the transfection of sensitive cells, yielding optimal transfection efficiencies and gene expression. Transfection with PromoFectin requires no medium change and it can be used for adherent and non-adherent cells. It compacts and protects the nucleic acid of interest, enables efficient transport into the cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor the delivery to the nucleus.
Special cell type specific PromoFectin variants are also available, which have been developed for the efficient but gentle transfection of cells such as endothelial cells (e.g. primary HUVECs), hepatocytes, macrophages, or neuronal cells.
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