AMSBIO announces the introduction of CloneCatcher™ Gold DH5G Electrocompent E. coli cells - a new product that exhibits extremely high electroporation efficiencies.
The new product exhibits extremely high electroporation efficiencies that approach the theoretical maximum of 3.4 x 1011 cfu/µg pUC19 DNA. Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic and epitope libraries. The limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E. coli. Electroporation is one method to efficiently introduce DNA into E. coli. CloneCatcher™ Gold cells meet the needs of scientists that are seeking to find rare clones or are building complex or metagenomic libraries. CloneCatcher™ Gold significantly outperforms all other commercially available Electrocompetent Cells when introducing ligated DNA. When the amount of input DNA is limited, CloneCatcher Gold enhances your likelihood of success. CloneCatcher™ Gold requires less topoisomerase-loaded vector and therefore can reduce your overall operating costs. Supplied in convenient single-use tubes - CloneCatcher™ Gold can also enhance your productivity as the whole protocol for use takes only 10 minutes.
For further information on CloneCatcher™ Gold please visit our Website.
With Iris 6 INFORS HT releases a new SCADA software package for parallel bioprocess control, which brings a fresh new look and a whole raft of new features.
With Iris 6 INFORS HT releases a new SCADA software package forparallel bioprocess control. Iris 6 brings a fresh new look and a whole raft of new features to one of themost flexible and user friendly bioprocess control software programs available today. A special versionfor use with Multitron incubation shakers allows comprehensive process validation.
“Beginners will love the modern design and the easy navigation”, says Dr. Tony Allman, productmanager INFORS HT. A new ribbon menu provides quick access to the bioreactor and parametersettings. A variety of graphical displays such as correlation and bar graphs allow an easy interpretationof process data. The new version allows the user to annotate on-line graphics while a fermentation isin progress and to time-stamp samples for easy reference.
Advanced features such as the feedback control using sequences allow the experienced user toexplore all possibilities of bioprocess control. The seamless integration of both analysed and calculatedprocess parameters gives the users the possibility to achieve a combined record of all process data.“The implementation of off-line analysed parameters like OD or protein yield in the batch record file isgreat, now I can record all data in one single file”, says Dr. Michael Kaempf, Project Leader BioprocessDevelopment, EMPA (Switzerland). An enhanced reporter module makes creation of batch tickets, data export and printing both comprehensive and simple.
The Iris Parallel Bioprocess Control Software is available in 3 different packages depending on the type of equipment. The same software provides high quality data acquisition and sophisticated controlstrategies from shake flask up to pilot production.
All software packages can be qualified for validation purposes relating to FDA CFR Part 11, 21.
PromoKine’s plant-derived cytokines and growth factors are free of animal components, serum traces, and antibiotics.
Growing importance of fields like stem cell research require cytokines and growth factors produced in a safe host organism that prevent the occurrence of unwanted cellular responses caused by high endotoxin levels and other bacterial or mammalian impurities. Many commercially available cytokines and growth factors have endotoxin levels and other impurities high enough to seriously affect cellular processes like proliferation or differentiation.
PromoKine’s plant-derived cytokines and growth factors are free of animal components, serum traces, and antibiotics. They are also free of human, animal or bacterial infectious agents and have an endotoxin content typically 20-times lower than proteins produced in E. coli. Thus, they avoid problems caused by too high endotoxin levels and improve critical applications, e.g. in stem cell research.
The patented vericyte® technology allows an optimization of medium for primary cells based on the use of various combinations of specific proliferation factors.
Our patented vericyte® technology allows an optimization of medium for primary cells based on the use of various combinations of specific proliferation factors. At the moment we offer two unique vericyte® Growth Media: one for natural killer cells (NK cells) and one for endothelial cells (ECs).
vericyte® NK Cell Growth Medium
animal component free medium
addition of vericyte® proliferation factors to basal medium resulted in an induction of NK cell proliferation with an expansion rate of over 50-fold
tested in ADCC (antibody-dependent cell-mediated cytotoxicity), Cytokine release assays and Natural Killing assays
cells can be cultured for 12 weeks
vericyte® EC Growth Medium
enables up to 7 additional PDs compared to standard EC Growth Medium (up to 16 fold more cells)
EC cultured in vericyte® EC Growth Medium retain primary cell markers CD31 and van Willebrand factor and express those during expansion
90% of the endothelial cells grown in vericyte® EC Medium are negative for senescence markers, regardless of the PD.
"MedoClav" is a small media autoclave with 7 liter working volumes, an integrated stirring-system for the sterilization of different media under the pressure of 1 up to 3 bars.
"MedoClav" is a small media autoclave with 7 liter working volumes, an integrated stirring-system for the sterilization of different media under the pressure of 1 up to 3 bars. medoClav has adjustable temperatures up to 143°C, integrated media sensor and a hold temperature of 60°C for the direct and sterile bottling of media. The stirrer of medoClav provides a homogeneous mixing of the liquids under predefined conditions and optimal strerilization. MedoClav can be supplemented with our tube pumps for semi-automated filling of sterile petri dishes and culture bottles and is best suitable for:
upcyte® Hepatocytes are genetically enhanced primary hepatocyte human cell strains in virtually unlimited amounts.
upcyte® Hepatocytes are genetically enhanced primary hepatocyte human cell strains in virtually unlimited amounts.
They proliferate without being immortalized, whilst retaining their primary donor specific characteristics. Medicyte can provide you with up to 2000 vials of cells from each human hepatocyte donor! With over 90% plateability, availability of multiple donors and expression of drug metabolizing enzymes and transporters, these cells are ideal for CYP induction, inhibition or metabolism studies as well as cytotoxicity and genotoxicity assays. Try out upcyte® Hepatocytes and various application kits and find out for yourself their multiple advantages and benefits!
For more information about the upcyte® technology and other upcyte® products visit: www.medicyte.com
The Optilab UT-rEX (UHPLC refractometer with EXtended range) is the first RI detector specifically designed for use with UHPLC systems using columns packed with small beads.
Using a combination of miniaturized components and cutting-edge semiconductor photodiode technology as well as proprietary computer algorithms, the UT-rEX presents a host of instrument firsts:
Besides UV, mass spectrometry, and evaporative light scattering, there has been no RI detector available for UHPLC applications until now. The linearity of the UT-rEX over a wide concentration range coupled with its sensitivity to samples that are not UV active, make it an exciting addition to UHPLC.
Considering the extremely small peak volumes of UHPLC, band broadening in the UT-rEX is kept to the absolute minimum, at less than 4 µL, as compared to 20-40 µL or more from other RI detectors. As a result, the superior resolution from UHPLC columns and instruments will not be compromised with the UT-rEX RI detector.
In addition, the instrument has no range or gain settings. The full range of instrument detection is always present, and the full sensitivity exists over the entire range. The small flow cell and proprietary temperature regulation enable stable RI baselines and signals which further enhance the detector’s sensitivity.
The new Optilab UT-rEX can be operated below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed down to 4°C or as high as 50°C. The Optilab UT-rEX is compatible with all UHPLC systems.
The XRS 20 Bioreactor System is a new single-use bioreactor system with unique agitation and control properties to improve cell culture performance.
The XRS 20 Bioreactor System is a new single-use bioreactor system with unique agitation and control properties designed for the cultivation of mammalian cells in suspension culture under controlled conditions and suitable for applications ranging from general life sciences research to seed train operations and full GMP production at the 2 to 20 liter scale.
Comprised of three components (XRS 20 Platform, XRS 20 Controller, and AllegroTM XRS 20 Biocontainers), the XRS System produces superior performance (as measured by higher density, better cell viability, and higher expression levels) compared to competitor rocker systems because its unique Bi-Axial agitation produces shorter mixing times and higher mass transfer properties.
Solentim has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development.
Solentim, the developer of tools for shortening the upstream cell line development workflow, has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development. The new product can be used to quickly and reliably screen hundreds of plates from limited dilutions via automated image capture and analysis. By offering unmatched speed, Cell Metric™ rapid is ideal for large-scale projects investigating the optimal conditions for cell growth, processing many thousands of clones per day with walk-away ease of use. It will be launched and displayed at the 2nd Annual Cell Culture and Bioprocessing Conference in London (4-5 November), while more details can also be found on the Solentim website.
Using Cell Metric™ rapid, images from an entire 96-well plate can be captured in just 45 seconds, offering a more than three-fold improvement over the standard Cell Metric™. Clear, high resolution images are captured right up to the well edge to generate accurate, comprehensive and reproducible results. This makes it ideal for a wide range of analyses including confluence determination, clone selection and colony growth formation. The Cell Metric™ rapid can also integrate with Solentim’s well-proven, temperature-controlled microplate stacker and loader. This space-saving system holds a batch of 10 plates and has been designed to increase throughput as part of a workflow that reduces user intervention, maximising the use of resources and time. To achieve the highest levels of automation, the Cell Metric™ rapid can integrate with a wide variety of third-party robot arms, to form a completely automated robotic work cell.
Cell Metric™ rapid joins a growing product portfolio that offers market-leading clone imaging with significantly better optics and automated detection capabilities than previous generation products. Existing users have reported shaving up to three months off the development of a new cell line when using the Cell Metric™, while the accuracy provided by the system’s high resolution, whole-well imaging maximises the number of single cells that can be positively identified as clonal.
Aaron Figg, Managing Director of Solentim commented, “Automated analysis systems have the potential to significantly improve commercial cell line development at every stage of the process, from calibration of the cell dispensing methods and liquid handling steps, to the clone selection and right through to the optimisation of growth conditions and scale-up ready for manufacture. Our customers continue to report significant improvements in their process quality, time and cost savings from incorporating Cell Metric™ into their workflows.”
96-well filter plates pre-filled with a family of chromatography sorbents for sorbent screening, or to optimize conditions in high throughput mode for protein purification.
AcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with Pall chromatography sorbents, to enable early chromatography sorbent screening for protein purification. Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption. Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using Pall 1 mL or 5 mL PRC prepacked columns filled with the same sorbents.
AcroPrep ScreenExpert plate packages offer:
Flexibility: One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents or two optimization plates: one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent - You select your preference. Easy Scale Up: Perform Design of Experiments (DoE) in 96-well plates and transfer to column chromatography. 1 mL or 5 mL PRC pre-packed columns filled with relevant sorbents allow convenient verification and small preparative work. Minimized Sample Consumption: 50 μL of sorbent per well, allowing small sample usage. 1 mL well volume and 100 to 500 μL working volume. Convenience: A disposable format in a convenient individual package. Compatible with manual multi-well plate vacuum manifold or robotic workstation-assisted operations.
The smart design of the Eppendorf DASbox makes the 4-fold units ideal for parallel processing of 4, 8, 12 or more bioreactors.
The smart design of the Eppendorf DASbox makes the 4-fold units ideal for parallel processing of 4, 8, 12 or more bioreactors. Together with the Eppendorf DASware® software solutions the DASbox supports process development following the Quality by Design (QbD) approach by providing comprehensive information management, integration of 3rd party analyzers, and Design of experiments (DoE) tools as well as remote access via PC, notebook, iPhone® and iPad®.
The DASbox requires only 7.5 cm (3 in) of bench space per bioreactor maximizing use of lab space while being expandable in 4-fold unit increments to increase capacity as needed. Small working volumes of 60 - 250 mL make it a perfect fit for clone/cell line screening, media optimization and small scale process development.
Suitable for microbiology and cell culture, the DASbox features advanced process control and precise monitoring of all critical process parameters.
Integrated Feeding and Monitoring: Variable speed pumps and standard pH and DO sensors (redox or level options and optical pH sensors available)
Liquid-free temperature control for easy handling
TMFC Gassing: Integrated mass flow-controlled gas mixing system for continuous mixing of Air, N2, O2 and CO2
LC Color Display: All vessels and parameters at a glance
More background reports, more interviews and as always, news from all the European regions: Discover our newly relaunched 2014 Autumn Issue, with in-depth reports on cancer immune therapy, biorefineries, biosimilars. more