AMSBIO announces the introduction of CloneCatcher™ Gold DH5G Electrocompent E. coli cells - a new product that exhibits extremely high electroporation efficiencies.
The new product exhibits extremely high electroporation efficiencies that approach the theoretical maximum of 3.4 x 1011 cfu/µg pUC19 DNA. Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic and epitope libraries. The limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E. coli. Electroporation is one method to efficiently introduce DNA into E. coli. CloneCatcher™ Gold cells meet the needs of scientists that are seeking to find rare clones or are building complex or metagenomic libraries. CloneCatcher™ Gold significantly outperforms all other commercially available Electrocompetent Cells when introducing ligated DNA. When the amount of input DNA is limited, CloneCatcher Gold enhances your likelihood of success. CloneCatcher™ Gold requires less topoisomerase-loaded vector and therefore can reduce your overall operating costs. Supplied in convenient single-use tubes - CloneCatcher™ Gold can also enhance your productivity as the whole protocol for use takes only 10 minutes.
For further information on CloneCatcher™ Gold please visit our Website.
Solentim has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development.
Solentim, the developer of tools for shortening the upstream cell line development workflow, has announced the release of Cell Metric™ rapid, a high speed imaging and analysis system for cell line development. The new product can be used to quickly and reliably screen hundreds of plates from limited dilutions via automated image capture and analysis. By offering unmatched speed, Cell Metric™ rapid is ideal for large-scale projects investigating the optimal conditions for cell growth, processing many thousands of clones per day with walk-away ease of use. It will be launched and displayed at the 2nd Annual Cell Culture and Bioprocessing Conference in London (4-5 November), while more details can also be found on the Solentim website.
Using Cell Metric™ rapid, images from an entire 96-well plate can be captured in just 45 seconds, offering a more than three-fold improvement over the standard Cell Metric™. Clear, high resolution images are captured right up to the well edge to generate accurate, comprehensive and reproducible results. This makes it ideal for a wide range of analyses including confluence determination, clone selection and colony growth formation. The Cell Metric™ rapid can also integrate with Solentim’s well-proven, temperature-controlled microplate stacker and loader. This space-saving system holds a batch of 10 plates and has been designed to increase throughput as part of a workflow that reduces user intervention, maximising the use of resources and time. To achieve the highest levels of automation, the Cell Metric™ rapid can integrate with a wide variety of third-party robot arms, to form a completely automated robotic work cell.
Cell Metric™ rapid joins a growing product portfolio that offers market-leading clone imaging with significantly better optics and automated detection capabilities than previous generation products. Existing users have reported shaving up to three months off the development of a new cell line when using the Cell Metric™, while the accuracy provided by the system’s high resolution, whole-well imaging maximises the number of single cells that can be positively identified as clonal.
Aaron Figg, Managing Director of Solentim commented, “Automated analysis systems have the potential to significantly improve commercial cell line development at every stage of the process, from calibration of the cell dispensing methods and liquid handling steps, to the clone selection and right through to the optimisation of growth conditions and scale-up ready for manufacture. Our customers continue to report significant improvements in their process quality, time and cost savings from incorporating Cell Metric™ into their workflows.”
96-well filter plates pre-filled with a family of chromatography sorbents for sorbent screening, or to optimize conditions in high throughput mode for protein purification.
AcroPrep ScreenExpert Plates are a family of 96-well filter plates filled with Pall chromatography sorbents, to enable early chromatography sorbent screening for protein purification. Using these plates, various ligands can be screened to determine best selectivity option, and many purification conditions can be quickly optimized, with minimal sample consumption. Purification conditions optimized in AcroPrep ScreenExpert plates can then be transferred and scaled using Pall 1 mL or 5 mL PRC prepacked columns filled with the same sorbents.
AcroPrep ScreenExpert plate packages offer:
Flexibility: One screening plate filled with MEP, HEA, and PPA HyperCel™ mixed-mode sorbents or two optimization plates: one filled with MEP HyperCel mixed-mode sorbent, and one filled with HyperCel STAR AX anion exchange sorbent - You select your preference. Easy Scale Up: Perform Design of Experiments (DoE) in 96-well plates and transfer to column chromatography. 1 mL or 5 mL PRC pre-packed columns filled with relevant sorbents allow convenient verification and small preparative work. Minimized Sample Consumption: 50 μL of sorbent per well, allowing small sample usage. 1 mL well volume and 100 to 500 μL working volume. Convenience: A disposable format in a convenient individual package. Compatible with manual multi-well plate vacuum manifold or robotic workstation-assisted operations.
The smart design of the Eppendorf DASbox makes the 4-fold units ideal for parallel processing of 4, 8, 12 or more bioreactors.
The smart design of the Eppendorf DASbox makes the 4-fold units ideal for parallel processing of 4, 8, 12 or more bioreactors. Together with the Eppendorf DASware® software solutions the DASbox supports process development following the Quality by Design (QbD) approach by providing comprehensive information management, integration of 3rd party analyzers, and Design of experiments (DoE) tools as well as remote access via PC, notebook, iPhone® and iPad®.
The DASbox requires only 7.5 cm (3 in) of bench space per bioreactor maximizing use of lab space while being expandable in 4-fold unit increments to increase capacity as needed. Small working volumes of 60 - 250 mL make it a perfect fit for clone/cell line screening, media optimization and small scale process development.
Suitable for microbiology and cell culture, the DASbox features advanced process control and precise monitoring of all critical process parameters.
Integrated Feeding and Monitoring: Variable speed pumps and standard pH and DO sensors (redox or level options and optical pH sensors available)
Liquid-free temperature control for easy handling
TMFC Gassing: Integrated mass flow-controlled gas mixing system for continuous mixing of Air, N2, O2 and CO2
LC Color Display: All vessels and parameters at a glance
The new BioBLU 1 vessels fill the gap between the BioBLU 0.3 mini-scale bioreactors and the larger BioBLU 5c.
The Eppendorf BioBLU family of rigid-walled stirred-tank single-use vessels is growing: The new BioBLU 1 vessels fill the gap between the BioBLU 0.3 mini-scale bioreactors and the larger BioBLU 5c. Available for cell culture (320 mL - 1.25 L working volume) and microbial applications (250 mL - 1.25 L) they perfectly add to the Eppendorf portfolio of scalable single-use solutions. Scientists working in cell culture and microbial process development benefit from BioBLU 1 through easy handling, minimized set-up time and the elimination of cleaning and sterilization steps. Parallel processing of 4, 8 or more vessels using DASGIP Control software shortens development times and reduces costs.
Parallel temperature control with the Eppendorf DASGIP® Bioblock (adaptor kits available)
Proven cell culture single-use design with pitched blade impellers
Fermentation vessel featuring Rushton-type impellers and innovative baffles for cooling
Liquid-free exhaust condensation with Peltier technology
PeproGrow-1 is designed to be used as a supplement to serum-free cell culture media formulations.
PeproGrow-1 (Catalog # 700-C100) is designed to be used as a supplement to serum-free cell culture media formulations that can sustain the growth of adherent mammalian cell lines, and has been tested with HEK-293, HeLa, and A549 cells. This kit could potentially improve the culturing conditions of other adherent cells, but suitability for cells other than those pre-tested is not guaranteed. PeproGrow-1 is protein-free, has a chemically defined composition, and does not contain any animal-derived materials. It is intended specifically to be used with DMEM/F12 basal media (Invitrogen catalog # 10565*) and contains enough material to supplement 10L of media.
The PromoCell MSC Growth Medium DXF eliminates the risks of using traditional FCS-containing MSC media and this innovative formulation provides significant benefits.
Chemically defined and humanized culture of MSC
Undifferentiated Mesenchymal Stem Cells (MSC) can be expanded in vitro to produce of large numbers of cells. For this expansion, traditional MSC culture media that contains special pre-qualified lots of fetal calf serum has been necessary. In addition to the serious ethical concerns relating to the use of FCS, this ill-defined component of animal origin is the major obstacle in providing consistent culture conditions and optimal performance of MSC.
The PromoCell MSC Growth Medium DXF eliminates the risks of using traditional FCS-containing MSC media. This innovative formulation provides significant benefits:
· Chemically defined
· Humanized: animal-free / xeno-free
· No adaption of cells required
· Outstanding expansion performance
· Maintenance of multipotency
· Supports primary isolation
· Standardized: minimal lot-to-lot variation
· Cost effective: more cells with less medium
For economical serum-free routine culture of MSC, the MSC Growth Medium DXF may be used in combination with bovine fibronectin as an ECM coating and our Detach Kit. For chemically defined culture conditions, the trypsin-containing DetachKit should be replaced by the Cell Dissociation Solution ACF. The use of human fibronectin and the Cell Dissociation Solution ACF completes the MSC Growth Medium DXF to yield a powerful animal-free and chemically defined complete culture system.
The packing materials and packed columns are an ideal choice for high performance liquid chromatography.
Extensively used in laboratories all over the world, our TSKgel is designed for researchers seeking the highest level of performance. Covering the total range of HPLC, this product is made from small, spherical particles and offers high resolution (even at high flow rates), excellent reproducibility, and long column life. Scaling up from analytical to preparative columns is made simple and easy.
The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).
By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.
This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.
Currently kits are available to:
• quantify and qualify NGS fragments. • quantify and qualify RNA. • quantify and qualify genomic DNA. • do Mutation Detection, also known as TILLiNG, a reverse genetics technique.
The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.
Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.
An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.
ForteBio announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems.
ForteBio, a division of Pall Life Sciences and a leading supplier of instrumentation for accelerated drug discovery and development, today announced the launch of the Octet® HTX system, the new flagship member of the Octet line of label-free interaction analysis systems. The Octet HTX system addresses the acute industry need for increased analysis speed and sample throughput when characterizing large numbers of therapeutic candidates.
Octet systems provide unique insights into drug-target binding interactions, insights that are used to fine-tune the affinity and specificity of drug molecules. The Octet HTX instrument performs kinetic analysis of up to 96 binding interactions simultaneously, a capability offered by no other stand-alone label-free platform. The system offers dramatic time savings for researchers studying large panels of drug candidates, and provides innovative data analysis tools for developers of monoclonal antibodies and other biotherapeutic entities. In addition, the Octet HTX performs full 96-well plate determinations of protein concentrations in as little as 2 minutes.
“The need for higher analytical throughput is a central theme with our pharmaceutical and biopharm customers,” noted Robert Wicke, General Manager at Pall’s ForteBio Division. “We believe researchers will be delighted by the Octet HTX system. The HTX system was engineered for speed while maintaining peak performance, and will directly address critical bottlenecks in the drug development process.”
The Octet HTX system was unveiled today at the BioProcess International 2013 conference in Boston, Mass.
Gel-free. Blot-free. Hands-free. A Simple Western is the Western you know well, only simplified.
Gel-free. Blot-free. Hands-free.
A Simple Western is the Western you know well, only simplified. It is not a workaround to the existing process, a Western-like substitute, or a partial solution. It's the real thing, completely reinvented. We at ProteinSimple think that's something to get excited about!
Get your time back. Load your samples in Simon, Sally or Peggy, press start and go enjoy your new hands-free time. Come back a few hours later to fully analyzed results. Get more consistency. People introduce variability—we arehuman after all. Simple Westerns are fully automated, so they just give you more reproducible data. Get truly quantitative. Since blot transfer of proteins isn't needed, you can get straight to real, quantitative results. Get more efficient. Sometimes you can only squeeze out enough sample for one experiment. Simple Westerns require a lot less protein and antibody so you have enough for those replicates.
• Learn about Simple Westerns • Learn about Peggy • Learn about Sally • Learn about Simon • Learn about NanoPro 1000 • Learn about Compass Software 21 CFR Part 11 Compliance • Simple Western Systems Comparison Chart
The Swiss Biotech Day is the leading biotechnology conference in Switzerland. This year, the event brought together more than 350 senior executives from the life science industry across Europe. Programme highlights on 8 April 2014 included the presentation of the Swiss biotech report by Jürg… more
The LiposoFast-Basic produces unilamellar liposomes by the manual extrusion of a multilamellar liposome suspension through a polycarbonate membrane of defined pore size, using gas-tight, glass syringes. more
Quality Audit - A Tool for Continuous Improvement and Compliance
The word “audit,” in the broadest sense, refers to a variety of activities. It may refer to an accounting firm examining the financial statements of a public corporation, or a consultant checking the process of lid sealant dispensing in a semiconductor package assembly line. more...