Product of the Week

Disposable PD-10 Desalting Columns
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GE-Healthcare

Disposable PD-10 Desalting Columns

Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.


  • Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex G-25 Medium. 
  • Flexible protocols allow runs by gravity flow or centrifugation. 
  • Easy to use and equilibrate; apply sample; elute. 
  • Preparation of the samples in the range of 1.0 to 2.5 ml by gravity flow and 1.75 to 2.5 ml with centrifugation. 
  • Desalt multiple samples in parallel with a desalting capacity > 90%. 
  • Recovery in the range 70 to >95%. 


The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.


Contact information:


Andrea Löhndorf, PhD, MBA
Marketing Manager EMEA
GE Healthcare Europe GmbH
Life Sciences
 
Mobil: +49(0) 174 92 36 400

andrea.loehndorf@ge.com

www.gelifesciences.com

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INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package for parallel bioprocess control, which brings a fresh new look and a whole raft of new features.

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package forparallel bioprocess control. Iris 6 brings a fresh new look and a whole raft of new features to one of themost flexible and user friendly bioprocess control software programs available today. A special versionfor use with Multitron incubation shakers allows comprehensive process validation.

“Beginners will love the modern design and the easy navigation”, says Dr. Tony Allman, productmanager INFORS HT. A new ribbon menu provides quick access to the bioreactor and parametersettings. A variety of graphical displays such as correlation and bar graphs allow an easy interpretationof process data. The new version allows the user to annotate on-line graphics while a fermentation isin progress and to time-stamp samples for easy reference.

Advanced features such as the feedback control using sequences allow the experienced user toexplore all possibilities of bioprocess control. The seamless integration of both analysed and calculatedprocess parameters gives the users the possibility to achieve a combined record of all process data.“The implementation of off-line analysed parameters like OD or protein yield in the batch record file isgreat, now I can record all data in one single file”, says Dr. Michael Kaempf, Project Leader BioprocessDevelopment, EMPA (Switzerland). An enhanced reporter module makes creation of batch tickets, data export and printing both comprehensive and simple.

The Iris Parallel Bioprocess Control Software is available in 3 different packages depending on the type of equipment. The same software provides high quality data acquisition and sophisticated controlstrategies from shake flask up to pilot production.

All software packages can be qualified for validation purposes relating to FDA CFR Part 11, 21.


Contact:

Dr. Tony Allman, Product Manager Fermentation

Infors AG

Rittergasse 27

4103 Bottmingen

Switzerland

Phone: +41 (0)61 425 77 00

Fax: +41 (0)61 425 77 01

e-Mail: t.allman@infors-ht.com

Web: www.infors-ht.com

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.

Trillium/Beckman Coulter

Beckman Coulter Life Sciences is expanding their flow cytometry portfolio with the addition of 14 new human monoclonal antibodies from Trillium Diagnostics LLC.  The analyte specific reagents (ASRs) provide high specificity, convenience and flexibility in flow cytometry.  A new cell permeabilization reagent is also being offered. 

The new monoclonals include human anti-hemoglobin F, which recognizes a fetal hemogloblobin molecule; CD163 markers for anti-inflammatory signal and disease process research and CD64 markers, which react with Fc gamma receptors. Characterized, quality controlled, convenient, liquid reagents allow users to spend less time in sample preparation and more time on experiments.

Rounding out the line is a monoclonal for MNDA (myeloid nuclear differentiation antigen). All antibodies are available unconjugated or conjugated to a selection of fluorochromes. Intra-Cell™ concentrate for cell permeabilization is also being released.

Beckman Coulter Life Sciences offers an extensive line of human and non-human antibodies as well as a range of supporting reagents and immune monitoring products. The new antibodies are supplied in 1.0 mL of buffer, are ready to use and facilitate flexibility in experimental design.

“Convenience and specificity are important features for scientists,” said Mario Koksch, vice president and general manager for Beckman Coulter Life Sciences’ Flow Cytometry Business Unit. “These new antibodies are directed at important targets and will improve accuracy for researchers.”

A strategic partnership between the companies enables Beckman Coulter Life Sciences to further diversify their extensive flow cytometry reagent portfolio and Trillium Diagnostics to broaden the reach of their products in the global market.    


Contact Information:

Hillary Hunter

Beckman Coulter Life Sciences Public Relations

hillarythunter@beckman.com

www.beckmancoulter.com

Advanced Analytical Technologies

The Fragment Analyzer™ by Advanced Analytical is a fluorescence-based capillary electrophoresis instrument for both sizing and quantifying nucleic acids (DNA and RNA).

Advanced Analytical Technologies

By using a sensitive intercalating dye coupled with a powerful LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers and can be used to separate dsDNA fragments and RNA (mRNA and total RNA). The Fragment Analyzer™ Automated CE System is the most flexible system on the market today, with the greatest sensitivity, the highest separation resolution over a wide range, the widest dynamic range and the fastest separation times.

This instrument comes available for customers who wish to run either 12 samples or 96 samples at a time.

Currently kits are available to:

• quantify and qualify NGS fragments.
• quantify and qualify RNA.
• quantify and qualify genomic DNA.
• do Mutation Detection, also known as TILLiNG, a reverse genetics technique.

The application kits help laboratories to streamline workflow and avoid bottlenecks typically found with competitive lab-on-a-chip methods. Gels formulated to provide high resolution separation are also available for general fragment analysis, such as SSR/microsatellites, small and large PCR fragments (amplicons) and resolve fragments sized between 10 bp and 40,000 bp with resolution down to 2bp.

Each and every kit is performance-tested prior to shipment to ensure quality, accuracy and ease of use for customers. Most kits require a single pipetting step before placement of the sample plate onto the instrument.

An analytical software package, PROSize™, greatly reduces post-separation analysis time. Put them all together — the instrument itself, the QC-tested application kits, and the analytical software — and the combination makes the Fragment Analyzer™ Automated CE System a powerful package for reducing labor and improving lab workflow and throughput.

Contact:

Advanced Analytical Technologies GmbH

Lutz Büchner

Im Neuenheimer Feld 583

69120 Heidelberg

Tel.: +49-(0)-6221-868058-20

lbuechner@aati-de.com

www.aati-de.com 

GE Healthcare

His Mag- and Ni Sepharose excel are immobilized metal ion affinity chromatography (IMAC) media (resins) designed for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.

GE Healthcare

Nickel ions are very strongly bound to both media, enabling direct loading of large sample volumes without removing agents that normally would cause metal ion stripping. Both media significantly simplify and speed up the workflow and are excellent choices when working with purification of histidine-tagged proteins secreted from eukaryotic cells. 

Key features:

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several different formats for screening and preparative purification of histidine-tagged proteins 

Background:

IMAC purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants has traditionally been challenging because of incompatibility between the IMAC medium and the cell culture medium. The cell culture medium strips the immobilized metal ions from the IMAC medium during sample loading, resulting in low or no binding of the target protein. The purification has been further complicated by the fact that the target protein concentration is often low. This requires the use of large sample volumes, which in turn might lead to increased metal ion stripping. Considerable sample pretreatment has been required to overcome these problems, an example being buffer exchange by diafiltration in combination with concentration procedures. Such pretreatment is time consuming, potentially harmful to sensitive proteins, and can cause unnecessary loss of target protein.


Contact Information:

GE Healthcare Europe GmbH

Life Sciences

Andrea Löhndorf, PhD, MBA

Regional Marketing Manager

Mobil: +49 (0)174 92 36 400

andrea.loehndorf@ge.com

www.gelifesciences.com

CETICS

AquaSpec mid-infrared spectroscopy creates digital metabolomic fingerprints of biological samples – in less than two minutes.

CETICS Healthcare Technologies GmbH

In contrast to conventional in-vitro diagnostic methods,  AquaSpec technology does not require sample preparation or consumables. It uses less than 100 microliters of sample volume and provides physicians with quantitative as well as qualitative information on important blood parameters – immediately and without time-consuming transport to diagnostic laboratories.

The core element of AquaSpec technology is the patented, ultrafine and high-precision flow cell especially developed for the measurement of aqueous biological samples. The flow cell is less than 10 µm thick, with a precision in the nanometer range, thus providing previously unrivalled measuring accuracy and reproducibility to exploit the full potential of modern mid-infrared spectroscopy. The high information content and digital archiving of the spectra open up completely new paths in analysis and diagnostics – also for retrospective studies, metabolomics studies, investigations, and the monitoring of disease and therapy processes.

Fields of application:

  • Toxicology
  • Cell Culture Quality Control
  • Pharmaceutical Development


Ask us for additional information:  

CETICS Healthcare Technologies GmbH

Schelztorstr. 54-56

73728 Esslingen am Neckar

Germany

Phone:+49 (0)711 300 655-0

Fax:+49 (0)711 300 655-29

e-mail: info@cetics-ht.com

BioTek

Cytation 3 is an automated digital microscope that provides high quality cellular imaging up to 60x in slides, microplates, Petri dishes and cell culture flasks.

BioTek

Cytation™ 3 cell imaging multi-mode system combines automated digital microscopy and conventional multi-mode microplate detection in one instrument. This unique patent pending design provides rich phenotypic cellular information with well-based quantitative data, bringing cell biology research to a new level.

Fully integrated solution for automated fluorescence and brightfield microscopy of slides, microplates and flasksTemperature control to 45°C, CO2/O2 gas control and orbital shakingPowerful yet easy to use software for efficient image capture and analysisEliminates inconveniences commonly associated with conventional microscopy

The modular platform can easily upgrade to add conventional multi-mode microplate detection, to provide well-based intensity data along with phenotypic information from the microscope.

DRIFTON

This is a highly advanced programmable pneumatic digital dispenser. It can store eight programmes and it can partially compensate for viscosity variation.

Drifton

The dispenser features new digital controller technology with a clear digital display for a simultaneous overview of all parameters.

The Drifton 206-DF dispenser can store eight programmes in the controller’s memory and when it is programmed, it will adjust the pneumatic pressure automatically as the liquid becomes thicker or thinner.

The dispenser can partially compensate for variation in the dispensed medium.

Remember to order an air fitting to go with this one-component dispenser.

Contact:

Drifton A/S
Avedøreholmen 84
DK-2650 Hvidovre
Tel.: +45 3679 0000
E-mail: info@drifton.dk 

Life Technologies

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs.

Life Technologies

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. SuperScript® III RT features:

  • Thermostability—half-life of 220 min at 50°C
  • Yield—reduced RNase H activity for more full-length cDNA
  • Specificity—full activity at 50°C for increased specificity with gene-specific primers
  • Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3´ and 5´ RACE

For further information please click here.

Porvair Sciences Ltd

Porvair Sciences will present its complete range of automation friendly microplates and equipment at European Lab Automation 2012 in Hamburg, Germany in partnership with long-term collaborator, KBiosystems.

Porvair Sciences Ltd

Visitors to the Porvair / KBio stand at ELA 2012 will be able to see the Universal Robotic Manifold plus a growing range of high quality reagent reservoirs. The Universal Robotic Manifold is designed to be easily assembled and disassembled by robotic manipulators, thus enabling productive automation of SPE or DNA clean-up procedures. Able to accommodate collection plates from 14mm - 44mm in height and adaptable for working with short, medium and long skirts and drip directors, the Universal Robotic Manifold can operate with many different brands of filter plate and collection trays. Supplied with an integral vacuum gauge, the Manifold provides complete control of vacuum pressure ensuring plates can be processed with high reproducibility.

Combining uncompromising high quality with an affordable price - Porvair Sciences will display its extensive range of deep well, shallow well, storage, assay, SPE, protein precipitation and filtration microplates precisely manufactured to comply with ANSI/SBS dimensions ensuring complete compatibility with all automated sample handling systems, microplate readers and washers. 

Also on display will be the Ultravap RC - Porvair's all new robot-compatible nitrogen blow down station. For many years, Porvair Sciences has led the field in nitrogen blow down evaporation. This new version of Porvair's most popular sample concentrator makes it even easier to integrate an automatic dry down step into your automated liquid handling protocol.

Contact:

Porvair Sciences Ltd

Dorset House, Regent Park

Kingston Road, Leatherhead

Surrey KT22 7PL  UK

Tel: +44-1372-824290

Fax: +44-1372-824271

int.sales@porvair-sciences.com

www.porvair-sciences.com

GE Healthcare

rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.

GE Healthcare

rProtein A/Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow.

•Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results. 

•Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs. 

•High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%. 

•High binding capacity such as Protein A/Protein G GraviTrap ~ 35 mg human IgG/column. 

rProtein A/Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein A and protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Contact Information:

GE Healthcare Europe GmbH
Life Sciences
Andrea Löhndorf, PhD, MBA
Regional Marketing Manager
cell: +49 (0)174 92 36 400
andrea.loehndorf@ge.com
www.gelifesciences.com

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No liability assumed, Date: 30.08.2015

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