Product

3D Culture Cell Harvesting Kit
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AMS Biotechnology

3D Culture Cell Harvesting Kit

The AMSBIO Cultrex® 3D Culture Cell Harvesting Kit is based on a non-enzymatic approach that prevents biochemical degradation during processing and overcomes the problems associated with extracting cells from 3D culture in BME or Laminin I.

The quality of the harvested cells is ideal for Western blotting and RNA and DNA analysis. In 3D culture, cells are grown in a microenvironment of specialized proteins that mimics in vivo conditions and allows the cells to organise into complex structures rather than uniform monolayers.



This new kit provides an optimised and standardised solution for the isolation and normalization of cell lysates from 3D Culture Matrix, BME or Laminin I for subsequent biochemical analysis.



3D Cultures exhibit cellular behaviours and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact or 3D grown cells from extracellular proteins comprising the 3D matrix. 

Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface, and protease activity may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerising extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers.

Contact:

AMS Biotechnology (AMSBIO)

184 Milton Park
Abingdon

Oxon OX14 4SE UK

Tel: +44-1235-828200 / +1-800-987-0985

Fax: +44-1235-820482


info@amsbio.com 

www.amsbio.com

PromoCell

MATra is an easy-to-handle, very fast and highly efficient technology for optimized transfection of cell lines and primary cells.

PromoCell GmbH

Magnet Assisted Transfection (MATra) is an easy-to-handle, very fast (15 minutes!) and highly efficient technology to transfect cells in culture. With MATra, all types of nucleic acids from plasmid DNA or siRNA to oligonucleotides can be delivered – even into hard-to-transfect cell types such as neuronal or stem cells. Using this new technique, nucleic acids are in a first associated with specific magnetic nanoparticles. A magnetic force is applied then and the nucleic acids will be quantitatively and quickly drawn towards and delivered into the target cells leading to efficient transfection without disturbing the membrane architecture and without causing chromosomal damage.  MATra can be used with adherent or suspension cells and is functional with or without serum. Compared to other transfection techniques (e.g. electroporation or lipofection), MATra results in extremely low cytotoxicity and is very cost-effective. MATra can also be adapted to high-throughput transfection assays using robotic stations and adapted protocols.

Our PromoFectin Transfection Reagent provides highly efficient and reproducible delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells. It consists of a non-liposomal polymer that is stable, water-soluble and free of any components of animal origin. PromoFectin compacts and protects the nucleic acid of interest, enables efficient transport into the cells via endocytosis and allows for a rapid and almost complete release of the intact nucleic acid into the cytosol – all of which favor the delivery to the nucleus. Transfection with PromoFectin results in very low toxicity and is therefore ideally suited for the transfection of very sensitive cells, yielding optimal effects on gene expression. It can be used for adherent and non-adherent cells and the protocol does not require a medium change. Special cell type-specific PromoFectin variants are also available, which have been developed for an optimized transfection of e.g. endothelial cells (HUVECs, HDMECs etc.), hepatocytes, macrophages, dendritic or neuronal cells.

In addition, PromoKine provides a range of small-sized reporter plasmids (beta-galactosidase, luciferase, GFP, CAT, and SEAP) as well as fusion plasmids, which allow high-level expression of a target gene of interest with a GFP or luciferase fusion tag at its C- or N-terminus. The corresponding reporter assays (luciferase, beta-galactosidase & GFP) are also available.


See our current MATra and PromoFectin promotions at www.promokine.info/promotion!


Contact:

PromoCell GmbH

Sickingenstrasse 63/65

69126 Heidelberg

Germany

+49 6221 649 34 0

www.promokine.info

info@promokine.info

WYATT

Revolutionise your performance by transforming your conventional, batch dynamic light scattering system into the stable, automated DynaPro Plate Reader.

Wyatt Technology Europe GmbH

Automated, high-throughput, temperature-controlled Dynamic Light Scattering

Revolutionise your performance by transforming your conventional, batch dynamic light scattering system into the stable, automated DynaPro™ Plate Reader. The DynaPro Plate Reader incorporates state of the art technology, providing superior filter-free high throughput dynamic light scattering (DLS or QELS) performance. The non-invasive technology measures samples directly from industry-standard microplates (96, 384, or 1536 well plates) using as little as 4 microliters of sample per well. After the plate is loaded into the DynaPro, the data are collected automatically, in an unattended manner, by the easy-to-use DYNAMICS® software, which supports full integration with industry leading liquid handling robotics. Once the data are collected the samples can be recovered and the plates discarded – no cleaning required!

Save valuable time and increase your productivity by factor 10 or more!

All in all, the DynaPro DLS Plate Reader will help you increase your productivity by a factor of 10 or more when sizing biomolecules and other nanoparticles compared to conventional batch DLS systems. With the DynaPro Plate Reader, you can perform the classic DLS applications but with more confidence. Samples can be loaded in duplicate or triplicate, and scanned several times by the Plate Reader, providing you with data that are a true measure of sample repeatability and reproducibility. Due to the low sample volumes and automated capability, these experiments can be performed in less time and without the hassles of conventional batch DLS. Moreover, because the DynaPro Plate Reader can operate in an unattended fashion, there is no additional ‘cost’ – your valuable time – associated with exploring a much larger number of solution conditions or protein or other nanoparticle constructs. The DynaPro can perform accelerated stability, thermal melting, and temperature studies of multiple samples in parallel.

New: The DynaPro Plate Reader is now available with Extended Temperature Control and On-board Camera! The onboard camera in the DynaPro Plate Reader II can automatically acquire and store images of each well, giving scientists a new dimension of data acquisition, and instant insight into the behavior of their samples.

For further information please click here.

Contact information:

Wyatt Technology Europe GmbH

Hochstrasse 18

56307 Dernbach

Germany

Telephone: +49-(0)2689-925-0

E-Mail: info@wyatt.eu

Life Technologies

This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time.

Life Technologies

From creating your gene to expressing your protein, Life Technologies offers a wide range of tools and expression systems that are easy to use, help enable rapid results, and offer high protein yields for every downstream application. This tool outlines the best products and services for your research needs, which can help save you weeks of hands-on time compared to using traditional methods. We even offer a complete service for the entire gene-to-protein workflow.

Request a copy of the Gene to Protein Brochure today by filling out the form here!

Or download the PDF version here!


Contactinformation for GeneArt® products and services:

Email: geneartsupport@lifetech.com

Europe: Tel: +49 (0)941 942 76100 Fax: +49 (0)941 942 76780

America and Asia: Tel: 800 955 6288 option 4/4/1

Advanced Analytical

The Oligo PRO™ 96XT system provides fast, automated and economical size-based purity analysis of single stranded DNA and RNA oligonucleotides, and double stranded RNA interference (RNAi) products.

Advanced Analytical

This dedicated analyzer is capable of analyzing 96 different samples simultaneously by parallel capillary gel electrophoresis with on-line, fixed wavelength UV absorbance detection. Preloaded and optimized separation and capillary conditioning methods simplify and streamline operation. The system can perform unattended analysis of up to 288 samples at once. Application-specific data analysis software provides automated or manual peak integration. The Oligo PRO™ is a proven technology used every day worldwide in production and research labs.


Key Features of the Oligo PRO™ System Include:

  • Low cost per sample.Unsurpassed throughput for oligo purity analysis!
  • Size-based, single nucleotide resolution from 10-mer to 80-mer lengths.
  • Easy to use and maintain with a low cost of ownership.
  • Minimal sample preparation and consumption.
  • DNA oligonucleotide and RNA oligonucleotide analysis.
  • New, native gel analysis for measuring purity and annealing efficiency of dsRNAi products.
  • A full line of available reagents for "turn-key" operation.


For further information please click here.

Eppendorf

The Reference 2 is the successor to the highly successful Reference pipette, and enhances the range’s already high standards with a new design, reduced weight and operating forces and multi-channel versions.

Eppendorf

As the premium product in Eppendorf’s pipette portfolio, the Reference 2 provides the most accurate results possible alongside robust, reliable handling and optimum user safety. These levels of security make the Reference 2 ideal for use with precious liquids, or in any application requiring extremely high levels of accuracy. When there can be no compromise on quality and reproducability, Eppendorf’s new Reference 2 is an ideal instrument for these liquid handling applications.

Applications:

  • Hide applications
  • Pipetting liquids
  • Volume determination
  • Mixing liquids
  • Filling of plates, gels, and reaction vessels
  • Phase extraction and removal of supernatants

Features:

  • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid an accidential tip ejection a clear haptic feedback is provided.
  • The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
  • The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
  • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids or other external conditions without the need for a full calibration.

For further information please click here.

Sartorius

BioPAT® SIMCA-online is a highly efficient software release for real-time multivariate statistical process monitoring and control of previously established Design Spaces.

Sartorius

Continuous real-time quality control and assurance is a highly desired state in biopharmaceutical manufacturing, which can be achieved by sophisticated process control strategies that use multivariate monitoring techniques to prevent or mitigate the risk of producing a poor quality product. Multivariate monitoring is based on models representing the relationship between parameters, whereas traditional process monitoring is based on univariate rules incapable of revealing correlation patterns and sometimes resulting in misleading conclusions.

BioPAT® SIMCA-online is a highly efficient software release for real-time multivariate statistical process monitoring and control of previously established Design Spaces based on current process parameters and (spectro) analytical data.

The software permits early detection of process deviations and provides user guidance for identifying potential root causes by displaying easy-to-understand graphics. This not only results in improved health, safety and environmental (HSE) performance, but also in enhanced control and assurance of the overall process and product quality.

Demo Versions you can find here!

Sartorius Stedim Biotech GmbH

August-Spindler-Strasse 11

37079 Göttingen, Germany

Phone: +49.551.308.0

Fax: +49.551.308.3289

info@sartorius-stedim.com

www.sartorius.de


Eppendorf

The electronic pipettes were specially designed for high professional standards to provide optimal support for you in your work.

Eppendorf

Simply better pipetting!


People who give 100 % every day deserve the best tool and the best equipment. You work on demanding problems, and important decisions depend on the results of your work. Your standard is extremely high and your performance is professional.

The electronic pipettes Eppendorf Xplorer and Eppendorf Xplorer plus were specially designed for high professional standards to provide optimal support for you in your work, with a new intuitive operating concept and design based on the proven Eppendorf PhysioCare Concept®.

These features set new standards for simplicity, precision andreproducibility, which means no more delays due to complicated programming or inflexible processes. Instead, you get precisely adjustable parameters, maximum reproducible results, fatigue-free work and consistent, full control over the pipetting processes.


For more information, please click here.

Tosoh Bioscience

The Immunoassay Analyser from Tosoh Bioscience offers the automation powers and performance of a large analyser in a bench top format.

Tosoh Bioscience

Random access allows any combination of tests, in any order at any time and a throughput of 36 results per hour with the first result in 20 minutes ensures a fast reliable service.


  • Will accept a variety of primary tubes with positive patient identification as well as positive test cup ID to eliminate errors.
  • Sample level sensing, to alert operator to short samples
  • Dual clot detection to maintain result integrity
  • Extremely simple operation
  • Unidirectional interface
  • Just 40 x 40 x 50cm and 30kg
  • Results are consistent and interchangeable between other Tosoh AIA analysers


The touchscreen, intuitive menus and onboard 'wizards' make the AIA-360 our easiest to use analyser to date.


Contact:

Tosoh Bioscience GmbH

Zettachring 6

70567 Stuttgart

Germany

Tel: +49-711-132570

Fax: +49-711-1325789

info.tbg@tosoh.com

www.tosohbioscience.com 

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package for parallel bioprocess control, which brings a fresh new look and a whole raft of new features.

INFORS HT

With Iris 6 INFORS HT releases a new SCADA software package forparallel bioprocess control. Iris 6 brings a fresh new look and a whole raft of new features to one of themost flexible and user friendly bioprocess control software programs available today. A special versionfor use with Multitron incubation shakers allows comprehensive process validation.

“Beginners will love the modern design and the easy navigation”, says Dr. Tony Allman, productmanager INFORS HT. A new ribbon menu provides quick access to the bioreactor and parametersettings. A variety of graphical displays such as correlation and bar graphs allow an easy interpretationof process data. The new version allows the user to annotate on-line graphics while a fermentation isin progress and to time-stamp samples for easy reference.

Advanced features such as the feedback control using sequences allow the experienced user toexplore all possibilities of bioprocess control. The seamless integration of both analysed and calculatedprocess parameters gives the users the possibility to achieve a combined record of all process data.“The implementation of off-line analysed parameters like OD or protein yield in the batch record file isgreat, now I can record all data in one single file”, says Dr. Michael Kaempf, Project Leader BioprocessDevelopment, EMPA (Switzerland). An enhanced reporter module makes creation of batch tickets, data export and printing both comprehensive and simple.

The Iris Parallel Bioprocess Control Software is available in 3 different packages depending on the type of equipment. The same software provides high quality data acquisition and sophisticated controlstrategies from shake flask up to pilot production.

All software packages can be qualified for validation purposes relating to FDA CFR Part 11, 21.


Contact:

Dr. Tony Allman, Product Manager Fermentation

Infors AG

Rittergasse 27

4103 Bottmingen

Switzerland

Phone: +41 (0)61 425 77 00

Fax: +41 (0)61 425 77 01

e-Mail: t.allman@infors-ht.com

Web: www.infors-ht.com

PromoCell

PromoKine’s plant-derived cytokines and growth factors are free of animal components, serum traces, and antibiotics.

PromoCell

Growing importance of fields like stem cell research require cytokines and growth factors produced in a safe host organism that prevent the occurrence of unwanted cellular responses caused by high endotoxin levels and other bacterial or mammalian impurities. Many commercially available cytokines and growth factors have endotoxin levels and other impurities high enough to seriously affect cellular processes like proliferation or differentiation.


PromoKine’s plant-derived cytokines and growth factors are free of animal components, serum traces, and antibiotics. They are also free of human, animal or bacterial infectious agents and have an endotoxin content typically 20-times lower than proteins produced in E. coli. Thus, they avoid problems caused by too high endotoxin levels and improve critical applications, e.g. in stem cell research.


Contact: 

PromoCell GmbH

Phone: +49 6221 649 34 0

www.promokine.info

info@promokine.info

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No liability assumed, Date: 01.10.2014


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