ItalyItaly

Toxicity Test to cut Animal Testing

09.04.2004

Milan/Rome - Italian researchers have developed a new cell culture method to test whether chemicals are toxic to humans. They engineered liver cells (hepatocytes) to secrete the human growth hormone (HGH) when exposed to toxic organic or inorganic compounds like Sodium arsenite, Benzopyrene, or Tetra-chloro hydro quinone. Additionally the transgenic hepatocyte cell line has been stably immortalized by insertion of the growth factor receptor gene c-met. As the novel cell lines can survive for longer than cultured primary liver cells, the cell type most commonly used in toxicity testing, adopting this new technique would reduce the number of animals used in such experiments.
To create the bi-transgenic cell line researchers around Marco Tripodi crossed two mice strains they previously created: A strain in which the gene for the human growth hormone (hGh) is constructed behind a heat-shock protein promotor (hsp70). Additionally, a mouse model allowing the reproducible immortalization of untransformed hepatocytes retaining complex liver functions.
To test their system the researchers added toxic arsenic and cadmium compounds to the cells and then looked for human growth hormone in the culture media. They found that even at low doses these compounds caused the cells to secrete the hormone - and were therefore deemed to be toxic. These low concentrations of the chemicals would not have been picked up by current toxicity-testing methods, which brand a chemical as ‚toxic' only if it kills liver cells.
The researchers believe that experiments using the transgenic cell lines will also be more informative than those using cultured liver cancer cell lines, the current alternative to cultured primary cells. Over time, liver cells in culture are known to alter their patterns of gene expression and no longer behave like liver cells in vivo. For example they often lose the ability to produce drug-activating and modifying enzymes. Cultured MMH-GH cell lines do not suffer from this problem, retaining features of liver cells inside the mouse. Moreover, if important changes do occur in these cells, fresh cell lines can be derived from the transgenic animals. The MMH-GH cell lines have a further advantage over current toxicity testing methods, according to the researchers. As they are derived from clonal cell populations assays using these cells should also be easier to standardize than current tests, which use heterogeneous primary cell cultures.

ItalyItaly

04.11.2010

Rome/Milan – Together with two Italian research institutes, British drug giant Glaxo­SmithKline has begun to develop a therapy for the heriditary immune defect ADA-SCID, which affects only 350 children worldwide. Fondazione...

ItalyItaly

03.11.2010

Rome – When it comes to GMOs, the gap between Italy’s federal government and its regions that has marked the country for years is rapidly widening into a gulf. The latest rupture was on display at the October session of the...

ItalyItaly

18.09.2010

Valletta – A new Life Sciences Park is to be created on the outskirts of Malta’s capital Valletta. The EUR20m project will provide space for companies operating in biotechnology and medical sciences. The park will be located in...

ItalyItaly

17.09.2010

Verona – Scientists at Glaxo­SmithKline in Verona have discovered that specific neurons in the amygdala control how mice react to fear stimuli. When they switched off the so-called Type I cells in the animals’ amyg­dala, the mice...

ItalyItaly

29.08.2010

San Michele all’Adige – The genetic code of the Golden Delicious apple has been deciphered. Led by Riccardo Velasco at the Edmund Mach Foundation in San Michele all’Adige in Italy’s Trentino province, a team of scientists from 20...

ItalyItaly

12.07.2010

: It’s hard to be a pro-GMO campaigner in Italy. Since 2001, the regions there have not implemented the plans for coexistence that were suggested by a ministerial decree. Instead, there was a de facto Italian moratorium on GM...

ItalyItaly

10.07.2010

Milan – One of Italy’s biggest biotech players wants to raise EUR58m to boost its pipeline of cancer therapies. The round will be closed on July 16th. With operating costs of EUR22.5m and a total loss of about EUR17m in 2009,...

ItalyItaly

10.07.2010

Milan – Italian researchers have restored sight to blind patients using stem cells from the patients’ own bodies. In a study published by the New England Journal of Medicine (10.1056/NEJMoa0905955), scientists from Modena and...

Displaying results 21 to 30 out of 181

< Previous 21-30 Next >

© 2007-2014 BIOCOM

http://www.european-biotechnology-news.com/nc/news/messages-archive/archive-italy/browse/2/article/toxicity-test-to-cut-animal-testing.html

Events

All Events

Stock list

All quotes

TOP

  • CHRONTECH PHARMA (S)0.09 SEK12.50%
  • WILEX (D)2.99 EUR6.79%
  • CO.DON (D)2.75 EUR5.77%

FLOP

  • ANTISOMA (UK)1.32 GBP-14.84%
  • XENETIC BIOSCIENCES (UK)6.00 GBP-14.29%
  • SERODUS (N)2.81 NOK-10.79%

TOP

  • WILEX (D)2.99 EUR251.8%
  • ADDEX (CH)3.82 CHF59.2%
  • 4SC (D)1.45 EUR49.5%

FLOP

  • HYBRIGENICS (F)1.72 EUR-29.5%
  • PROSENSA (NL)9.72 USD-23.4%
  • PROTHENA PLC (IE)17.37 USD-22.1%

TOP

  • SANTHERA (CH)64.00 CHF3022.0%
  • GW PHARMACEUTICALS (UK)426.00 GBP654.0%
  • PAION (D)2.67 EUR292.6%

FLOP

  • EVOCUTIS (UK)0.21 GBP-92.2%
  • CYTOS (CH)0.28 CHF-91.8%
  • THROMBOGENICS (B)9.14 EUR-70.6%

No liability assumed, Date: 29.07.2014


Current issue

All issues

Product of the week

Products